A new optical fibre feed-through for use in subsea in-well optical fibre sensing systems is reported. The new feed-through is compatible for use with standard subsea Christmas Tree penetrators and allows multiple re-mating of the feed-through over the lifetime of the device. The system has been extensively tested under in-well conditions and found to conform to the performance requirements. The new feed-through is planned to be used in one of the first subsea optical fibre in-well sensing systems.