1757-899X/452/2/022141
Abstract
A mild and novel process including on one-step chromatography was developed for the preparation of transferrin reference material with high purity.And function from Cohn fraction IV, by product of the Cohn fraction process.The method we have established is simple in operation and easy to enlarge.The transferrin product obtained with purity greater than 93% and a yield above 85%. Then the Tf secondary structure as well as function evaluated by circular dichroism (CD) proved no significantly varied.
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