LF

Before application

Immediately after application

5 min after application

10 min after application

15 min after application

Before application

Immediately after application

n.s.

5 min after application

**

n.s.

10 min after application

**

**

n.s.

15 min after application

**

**

n.s.

n.s.

MW

Before application

Immediately after application

5 min after application

10 min after application

15 min after application

Before application

Immediately after application

n.s.

5 min after application

n.s.

**

10 min after application

n.s.

**

n.s.

15 min after application

n.s.

**

n.s.

n.s.