Yeqin Kong, Axel A. Elling, Beibei Chen, Xingwang Deng
DOI: 10.4236/ajps.2010.12009   PDF    HTML     9,050 Downloads   20,986 Views   Citations

Abstract

Here, we analyzed whether the microRNA (miRNA) expression levels differ between maize inbred lines B73 and Mo17 and their reciprocal hybrids under salt and drought stress. We found that miR156, miR164, miR166, miR168, miR171 and miR319 are differentially expressed under abiotic stress. Interestingly, Mo17 × B73 showed the strongest change in miRNA expression in response to salt or drought stress, and was also the most resilient line when under abiotic stress in terms of water loss. In summary, our findings open the possibility that differential miRNA expression levels might be involved in heightened stress tolerance in maize hybrids.

Keywords

microRNA, Maize, Hybrid, Inbred, Salt Stress, Drought

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Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	M. W. Jones-Rhoades, D. P. Bartel and B. Bartel, “MicroRNAs and Their regulatory Roles in Plants,” Annual Review of Plant Biology, Vol. 57, January 2006, pp. 19-53.
[2]	D. H. Jeong, M. A. German, L. A. Rymarquis, S. R. Thatcher and P. J. Green, “Abiotic Stress-Associated miRNAs: Detection and Functional Analysis,” Methods in Molecular Biology, Vol. 592, January 2009, pp. 203-230.
[3]	D. P. Bartel, “MicroRNAs: Genomics, Biogenesis, Mechanism, and Function,” Cell, Vol. 116, January 2004, pp. 281-297.
[4]	S. Griffiths-Jones, H. K. Saini, S. van Dongen and A. J. Enright, “miRBase: Tools for microRNA Genomics,” Nucleic Acids Research, Vol. 36, January 2008, D154-D158.
[5]	E. Mica, L. Gianfranceschi and M. E. Pe, “Characterization of Five microRNA Families in Maize,” Journal of Experimental Botany, Vol. 57, July 2006, pp. 2601-2612.
[6]	L. Zhang, J-M. Chia, S. Kumari, J.C. Stein, Z. Liu, et al., “A Genome-Wide Characterization of microRNA Genes in Maize,” PLoS Genetics, Vol. 5, November 2009, e716.
[7]	X. Chen, “MicroRNA Biogenesis and Function in Plants,” FEBS Letters, Vol. 579, August 2005, pp. 5923-5931.
[8]	A. C. Mallory and H. Vaucheret, “Functions of microRNAs and Related Small RNAs in Plants,” Nature Genetics, Vol. 38, May 2006, S31-S36.
[9]	L. I. Shukla, V. Chinnusamy and R. Sunkar, “The Role of microRNAs and Other Endogenous Small RNAs in Plant Stress Responses,” Biochimica et Biophysica Acta, Vol. 1779, November 2008, pp. 743-748.
[10]	G. H. Shull, “The Composition of a Field of Maize,” American Breeders Association, Vol. 4, January 1908, pp. 296-301.
[11]	K. P. Kollipara, I. N. Saab, R. D. Wych, M. J. Lauer and G. W. Singletary, “Expression Profiling of Reciprocal Maize Hybrids Divergent for Cold Germination and Desiccation Tolerance,” Plant Physiology, Vol. 129, June 2002, pp. 974-992.
[12]	P. Rhode, D. K. Hincha and A. G. Heyer “Heterosis in the Freezing Tolerance of Crosses between Two Arabidopsis Thaliana Accessions (Columbia-0 and C24) that Show Differences in Non-Acclimated and Acclimated Freezing Tolerance,” Plant Journal, Vol. 38, June 2004, pp. 790-799.
[13]	K. Yamaguchi-Shinozaki and K. Shinozaki, “A Novel Cis-Acting Element in an Arabidopsis Gene is Involved in Responsiveness to Drought, Low-Temperature, or High-Salt stress,” Plant Cell, Vol. 6, February 1994, pp. 251-264.
[14]	C. Zhu, D. Schraut, W. Hartung and A. R. Sch?ffner, “Differential Responses of Maize MIP Genes to Salt Stress and ABA,” Journal of Experimental Botany, Vol. 56, November 2005, pp. 2971-2981.
[15]	E. Varallyay, J. Burgyan and Z. Havelda, “MicroRNA Detection by Northern Blotting Using Locked Nucleic Acid Probes,” Nature Protocol, Vol. 3, January 2008, pp. 190-196.
[16]	M. D. Abramoff, P. J. Magelhaes and S. J. Ram, “Image Processing with ImageJ,” Biophotonics International, Vol. 11, 2004, pp. 26-42.
[17]	S. Lu, Y. H. Sun and V. L. Chiang, “Stress-Responsive microRNAs in Populus,” Plant Journal, Vol. 55, July 2008, pp. 131-151.
[18]	K. J. Livak and T. D. Schmittgen, “Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-DDCT Method,” Methods, Vol. 25, December 2001, pp. 402-408.
[19]	G. Wu and R. S. Poethig, “Temporal Regulation of Shoot Development in Arabidopsis thaliana by miR156 and Its Target SPL3,” Development, Vol. 133, August 2006, pp. 3539-3547.
[20]	A. C. Mallory, B. J. Reinhart, M. W. Jones-Rhoades, G. Tang, P. D. Zamore, M. K. Barton and D. P. Bartel, “MicroRNA Control of PHABULOSA in Leaf Development: Importance of Pairing to the microRNA 5′ Region,” EMBO Journal, Vol. 23, July 2004, pp. 3356-3364.
[21]	H. S. Guo, Q. Xie, J. F. Fei, and N. H. Chua, “MicroRNA Directs mRNA Cleavage of the Transcription Factor NAC1 to Downregulate Auxin Signals for Arabidopsis Lateral Root Development,” Plant Cell, Vol. 17, April 2005, pp. 1376-1386.
[22]	H. Vaucheret, A. C. Mallory and D. P. Bartel, “AGO1 Homeostasis Entails Coexpression of MIR168 and AGO1 and Preferential Stabilization of miR168 by AGO1,” Molecular Cell, Vol. 22, April 2006, pp. 129-136.
[23]	C. Llave, Z. Xie, K. D. Kasschau and J.C. Carrington, “Cleavage of Scarecrow-Like mRNA Targets Directed by a Class of Arabidopsis miRNA,” Science, Vol. 297, September 2002, pp. 2053-2056.
[24]	N. Lauter, A. Kampani, S. Carlson, M. Goebel and S. Moose, “MicroRNA172 Down-Regulates Glossy15 to Promote Vegetative Phase Change in Maize,” Proceedings of the National Academies of Science USA, Vol. 102, May 2005, pp. 9412-9417.
[25]	C. Schommer, J. F. Palatnik, P. Aggarwal, A. Chételat, P. Cubas, E.E. Farmer, U. Nath and D. Weigel, “Control of Jasmonate Biosynthesis and Senescence by miR319 Targets,” PLoS Biology, Vol. 6, September 2008, p. e230.
[26]	R. Sunkar, A. Kapoor and J.K. Zhu, “Posttranscriptional Induction of Two Cu/Zn Superoxide Dismutase Genes in Arabidopsis is Mediated by Downregulation of miR398 and Important for Oxidative Stress Tolerance,” Plant Cell, Vol. 18, July 2006, pp. 2051-2065.
[27]	R. Sunkar and J.K. Zhu, “Novel and Stress-Regulated microRNAs and Other Small RNAs from Arabidopsis,” Plant Cell, vol. 16, August 2004, pp. 2001-2019.
[28]	R. Sunkar, X, Zhou, Y. Zheng, W. Zhang and J.K. Zhu, “Identification of Novel and Candidate miRNAs in Rice by High Throughput Sequencing,” BMC Plant Biology, Vol. 8, February 2008, p. 25.
[29]	B. Zhao, R. Liang, L. Ge,W. Li, H. Xiao, H. Lin, K. Ruan and Y. Jin, “Identification of Drought-Induced microRNAs in Rice,” Biochemical and Biophysical Research Communications, Vol. 354, March 2007, pp. 585-590.
[30]	D. Ding, L. Zhang, H. Wang, Z. Liu, Z. Zhang and Y. Zheng, “Differential Expression of miRNAs in Response to Salt Stress in Maize Roots,” Annals of Botany, Vol. 103, January 2009, pp. 29-38.
[31]	H. Vaucheret, “Plant ARGONAUTES,” Trends in Plant Science, Vol. 13, July 2008, pp. 350-358.
[32]	X. M. Chen, “A microRNA as a Translational Repressor of APETALA2 in Arabidopsis Flower Development,” Science, Vol. 303, March 2004, pp. 2022-2025.
[33]	J. Fernandes, D. J. Morrow,P. Casati and V. Walbot, “Distinctive Transcriptome Responses to Adverse Environmental Conditions in Zea mays L.” Plant Biotechnology Journal, Vol. 6, October 2008, pp. 782-798.