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Harnessing Natural and Synthetic Compounds for Lung Cancer Prevention For Cancer Prevention Awareness Month's conclusion, we are highlighting a recent study in the Journal of Cancer Research Communications on combining Artemisia Judaica extract and 1,2,4-triazole derivatives, showing enhanced lung cancer treatment potential by boosting immunity, reducing inflammation, and triggering cancer cell death. The NanoPhotometer® NP80 was used in this study to determine the concentration and purity of RNA. The RNA purity was assessed based on the A260/A280 ratio, with values between 1.8 and 2.0 indicating high-quality RNA. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Abdel Zidane, Elsayed Abdel Rehem Mahmoud, Amira Hamisa and El-Hassan Mokhamer #Implen #NanoPhotometer #UV/Vis #Spectrophotometer #RNA  #NP80 #CancerPrevention #LungCancerAwareness #CancerResearch #InnovativeTherapies #NaturalMedicine #ScientificBreakthrough #ArtemisiaJudaica #CancerTreatment #HealthcareInnovation #MedicalResearch #IntegrativeMedicine #CancerAwarenessMonth #OncologyResearch #FutureOfMedicine https://lnkd.in/dJ_3zPxZ

A productive morning at the Ecosciences Precinct, Dutton Park! Today, we hosted an information morning tea, showcasing our laboratory range to the brilliant teams at CSIRO and the Department of Agriculture and Fisheries (Queensland) It was a fantastic opportunity to connect with key players in government research and science and highlight how our products support their vital work. Thank you to everyone who attended, and we look forward to continuing to support your innovative projects! https://lnkd.in/gYQyjUyh Gyrozen ImplenSnijders Labs Temprecord International Ltd #EcosciencesPrecinct #CSIRO #LabSolutions #InnovationInScience #Opira

Harnessing the Power of the Immune System: How TRAIL Could Transform Cancer Prevention & Treatment Continuing on with February marking Cancer Prevention Awareness Month, this week’s Implen NanoPhotometer Journal Club is highlighting a study on how TNF-related apoptosis-inducing ligand (TRAIL) can shift immune cells toward a tumor-fighting state, offering promising insights into immunotherapy strategies that could aid in both cancer prevention and treatment. The study by Gunalp et. al. explored the role of TNF-related apoptosis-inducing ligand (TRAIL) in shaping macrophage behavior within tumors. Macrophages, immune cells that can either fight or support tumors, are influenced by TRAIL. This work demonstrated that TRAIL pushes macrophages toward the pro-inflammatory, tumor-fighting M1 type while reducing the presence of the tumor-supporting M2 type. It was shown in human macrophages that TRAIL boosts M1 markers at both the genomic and proteomic level while reducing M2 markers. This shift enhances the ability of macrophages to kill cancer cells, particularly in acute myeloid leukemia (AML). The study also linked higher TRAIL expression in tumors to better survival rates in cancer patients with a high number of macrophages. The mechanism elucidated in this work involved TRAIL binding to two receptors, DR4 and DR5, both of which contribute to the macrophage shift. TRAIL's ability to convert M2 macrophages into M1 macrophages suggests potential for improving cancer immunotherapy. These findings highlight TRAIL as a promising target to enhance anti-tumor immunity by modifying macrophage behavior in the tumor microenvironment. The NanoPhotometer was used in this study to check the purity of RNA isolated from macrophages. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Sinem Gunalp, Derya Göksu Helvacı, Aysenur Oner, Ahmet BURSALI, Alessandra Conforte, Huseyin Guner, Gökhan KARAKÜLAH, Eva Szegezdi and Duygu SAĞ. #Implen #NanoPhotometer #Spectroscopy #RNA #MacrophageActivation #CancerPrevention #Immunotherapy #TRAIL #CancerAwareness #FebruaryCancerPreventionMonth #Macrophages #CancerResearch #Oncology #TumorImmunity #HealthInnovation #PrecisionMedicine #MedicalBreakthroughs #Biotech #ImmuneBoost #LifesavingScience

"Targeted Breast Cancer Therapy with Nanoparticles and Brazilian Red Propolis" February is National Cancer Prevention Month, highlighting the importance of research in understanding and combating cancer. This week’s Implen NanoPhotometer Journal Club is highlighting a recent study published by Justino et. al. in the Journal of Drug Delivery Science and Technology that explored a new approach to breast cancer treatment using nanoparticles loaded with Brazilian red propolis extract (BRPE).  Propolis is a natural substance made by bees, known for its anti-cancer properties. However, in its raw form, it has limitations like poor stability and low absorption in the body. To overcome these issues, researchers encapsulated BRPE into polymeric nanoparticles, making it more effective. This study demonstrated that these nanoparticles (NCBRPE) were more toxic to breast cancer cells (MCF-7) but less harmful to normal breast cells (MCF-10), indicating a targeted effect. The nanoparticles also worked better in acidic tumor environments, making them even more promising for cancer therapy. Using advanced imaging techniques, it was confirmed that the nanoparticles were well-formed and successfully carried the BRPE extract. When tested in both 2D and 3D lab models, NCBRPE consistently showed stronger anti-cancer activity compared to free BRPE. Additionally, its small size allowed it to accumulate better in tumor tissues while minimizing damage to healthy cells. It was shown that NCBRPE was well tolerated in blood at lower doses, though higher concentrations caused some red blood cell damage. In addition, the nanoparticles activated the immune system’s complement pathway, indicating further studies are needed to refine their design for safe clinical use. Overall, NCBRPE was shown to be a promising candidate for breast cancer treatment that warrants further investigation. The NanoPhotometer was used in this study to measure hemolysis at 545 nm. The absorbance at 545 nm allowed for quantification of the amount of free hemoglobin in the supernatant, providing an indication of red blood cell membrane integrity after exposure to the nanoparticles (NCBRPE and NC). This helped determine the biocompatibility and safety of the nanoparticles, ensuring that they do not cause excessive damage to red blood cells at therapeutic doses. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Isabela Justino, Iasmin Ferreira, Caroline E. Apolinário Botteon, Luiz Fernando Tucci, Andréia Marincek Luzenti, Robson Amaral, Jennyfer Andrea Aldana Mejía, Yasmin Gonçalves, Jairo Bastos, Cleni Mara Marzocchi Machado and Priscyla Daniely Marcato Gaspari. #NanoPhotometer #JournalClub

The Impact of Altitude Hypoxia on Skiers: New Insights from Urinary Biomarkers With ski season in full swing across the Alps, athletes and enthusiasts are embracing the exhilaration of high-altitude slopes. However, the reduced oxygen levels at these elevations—altitude hypoxia—can trigger a range of physiological responses, including effects on the urinary system. This week’s Implen NanoPhotometer Journal club is highlighting a recent study by Pilato et. all, Urineprint of High-Altitude, published in Biophysical Chemistry, explores how urinary biomarkers and extracellular vesicles (EVs) can provide crucial insights into how the body adapts to hypoxic stress. Altitude hypoxia can lead to fluid imbalance, kidney function alterations, and metabolic shifts, all of which are particularly relevant for those spending extended periods in high-altitude environments. By analyzing the bio-physical-chemical properties of urinary EVs, researchers identified distinct biomolecular signatures, or "urineprints," that reflect physiological changes in response to hypoxia. These findings could improve early detection of altitude-related health risks, offering potential interventions to help high-altitude skiers and mountaineers optimize performance and recovery. Understanding these biological markers may enable better hydration and acclimatization strategies, reducing altitude sickness and dehydration risks—common concerns for skiers tackling long days on the slopes. As high-altitude research advances, insights from urinary biomarkers could prove invaluable for both athletes and medical professionals, ensuring a safer and more sustainable experience in alpine environments. The NanoPhotometer NP80 was used for determining the nucleic acid to protein ratio (NPr), estimated by UV absorbance at 260 nm and 280 nm by UV–vis spectrophotometry with 1 μl of sample. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Serena Pilato, Simona Mrakic-Sposta, Vittore Verratti, Carmen Santangelo, Carmen Santangelo, Stefano di Giacomo, Samanta Moffa, Antonella Fontana, Tiziana Pietrangelo, Federica Ciampini, Sofia Bonan, Pamela Pignatelli, Carmine Noce, Pietro Di Profio, Michele Ciulla, Danilo Bondi and Fabrizio Cristiano. #NanoPhotometer #JournalClub

"Liver Health and Lipid Balance: Insights for a Healthier New Year" As we embrace the New Year, many of us are setting goals to eat healthier, lower fat and cholesterol intake, and enhance overall well-being. This week's Implen NanoPhotometer Journal Club: New Year's Edition highlights an intriguing study by Bradić et al. that helped elucidate the liver's complex role in fat processing, emphasizing the critical interplay of enzymes and specialized cells in managing cholesterol and preventing liver damage. This study focused on metabolic dysfunction-associated steatotic liver disease (MASLD), a condition often linked to obesity or diabetes, where excess fat accumulates in the liver. This research explored the role of lysosomal acid lipase (LAL), an enzyme essential for breaking down fats in the liver. When LAL function is impaired, fat accumulation triggers inflammation and other complications. In experiments with mice fed a high-fat, high-cholesterol diet, the team observed significant fat buildup, inflammation, and scarring (fibrosis) in the liver. Removing Kupffer cells—liver-resident macrophages responsible for clearing debris—exacerbated these issues, intensifying fat accumulation and cholesterol imbalances. Interestingly, while removing Kupffer cells reduced some inflammation markers, it did not prevent liver damage, highlighting their dual role: aiding cleanup but potentially contributing to damage under certain conditions. This study underscores the delicate balance between enzymes and liver cells in maintaining liver health and points to promising avenues for developing treatments for conditions like MASLD. The Implen NanoPhotometer N60 was used in this research to measure peptide concentrations. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Ivan Bradić, Katharina Küntzel, Anita Pirchheim, Silvia Raineri, Birgit Schwarz, Michael Trauner, Martin Larsen, Nemanja Vujić and Dagmar Kratky #NanoPhotometer #JournalClub

"New Year, Healthier You: How Daphnia magna Studies on BPA Inspire Cutting Microplastics for Better Health" Kickstart the new year with a resolution to protect our planet and health—join us for this week’s Implen Nanophotometer Journal Club: New Year's edition, where we'll explore the impacts of microplastics. In a study just published this year by Sreevidya CP et. al. in the journal of Aquatic Toxicology, a new lab model was introduced using Daphnia magna cell cultures to explore the toxic effects of chemicals on aquatic ecosystems, focusing on Bisphenol A (BPA). BPA, commonly used in plastics, is widely present in the environment and known for its harmful impacts, including endocrine disruption and cellular damage.  Tests with BPA revealed significant effects including oxidative stress, increased activity of detoxification-related enzymes, and DNA damage. Additionally, gene analysis indicated that BPA exposure triggered an overexpression of stress and detoxification genes, while suppressing antioxidant defense genes. This study highlights the value of Daphnia magna cell cultures as a more sustainable and ethical alternative to traditional whole-organism tests. These findings align with the growing adoption of New Approach Methods (NAMs), which reduce reliance on animal testing in ecotoxicology. By providing a deeper understanding of BPA's molecular effects, this model supports ongoing efforts to evaluate the impacts of environmental pollutants while promoting more humane scientific practices. This innovative approach marks a significant step forward in studying and mitigating chemical risks in aquatic environments. The Implen NanoPhotometer NP80 was used in this research to determine the RNA concentration by measuring absorbance at 260/280 nm. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Sreevidya CP,  Manoj Kumar, Soumya Balakrishnan, Suresh Kunjiraman, Manomi Sarasan, Jason Magnuson and Jayesh Puthumana #NanoPhotometer #JournalClub

"Prioritizing Sleep: A New Year’s Resolution for Better Health" This week’s Implen NanoPhotometer Journal Club: New Year’s Edition focuses on the transformative health benefits of prioritizing sleep as a New Year’s resolution, inspired by a recent research study recently published by Huang et. al. in the International Journal of Molecular Sciences linking improved sleep hygiene to better gut health, reduced inflammation, and lower cardiovascular risks, including those associated with obstructive sleep apnea syndrome (OSAS). This study highlighted how poor-quality sleep, particularly in children with OSAS, disrupts essential bodily functions, leading to gut microbiome imbalances and increased cardiovascular risk. Additionally, harmful bacteria such as Acinetobacter and Klebsiella were found to be more prevalent in children with elevated blood pressure, further promoting inflammation and poor vascular health. Adopting sleep hygiene practices—such as maintaining consistent sleep schedules, reducing screen time, and creating a restful environment—can mitigate these effects. Quality sleep fosters gut microbiota balance, reduces inflammation, and supports cardiovascular health. This research emphasized how poor sleep hygiene exacerbates health risks, while good sleep habits can protect against conditions like hypertension and systemic inflammation. Improving sleep hygiene could positively influence gut health, reduce inflammation, and protect against long-term health issues, reinforcing the critical connection between sleep quality and systemic well-being, making it a resolution to consider for the New Year. The Implen NanoPhotometer was used in this research to assess the quality and quantity of DNA. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Chung-Guei Huang, Wan-Ni Lin, Li-Jen Hsin, Tuan-Jen Fang, Hsueh-Yu Li, Chin-Chia Lee and Li-Ang Lee #NanoPhotometer #JournalClub

"Carnosine and Salt Reduction: A New Year’s Path to Better Vascular Health" This week’s Implen NanoPhotometer Journal Club: New Year’s Edition explores how carnosine supplementation could assist with a New Year’s resolution to reduce salt intake by mitigating the vascular damage caused by high-salt diets and enhancing overall cardiovascular health. High salt consumption is a well-documented contributor to oxidative stress, impairing blood vessel function and elevating cardiovascular risks. A recent study by Drenjančević et. al. published in the journal of Nutrients investigated the protective effects of carnosine, a natural antioxidant, in rats on high-salt diets. The research divided rats into four groups: normal diet, high-salt diet, normal diet with carnosine, and high-salt diet with carnosine. The results demonstrated that carnosine improved blood vessel relaxation, reduced oxidative stress, and boosted antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx). It also activated protective cellular pathways involving NRF2 and HIF-1α, which are vital for combating oxidative damage and maintaining vascular integrity. Carnosine supplementation further prevented harmful protein oxidation and preserved antioxidant capacity, showcasing its potential as a dietary aid to counterbalance the effects of salt consumption. As we embrace the New Year, this research provides an encouraging perspective on integrating carnosine into wellness strategies to help achieve goals for healthier, low-salt living. The Implen NanoPhotometer was used in this work to determine the concentration and purity of RNA. The NanoPhotometer N60 was used to measure the peptide concentration. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Ines Drenjančević, Ana Stupin, Ivana Jukić, Nikolina Kolobarić, Petar Šušnjara, Natasa Kozina, Lora Kovač and Zrinka Mihaljević #NanoPhotometer #JournalClub

"Unwrapping the Microbial Mystery: A Quantum Leap in Metaproteomics with uMetaP" This week's Implen Nanophotometer Journal Club focuses on the work of Xian et. al. making a quantum leap in metaproteomics with the Ultra-sensitive metaproteomics (uMetaP) platform. Designed for ultra-sensitive detection and single-bacterium resolution, uMetaP unveils the hidden world of the microbiome with unprecedented precision and speed.   Metaproteomics studies the proteins of microbial communities, crucial for understanding how microbes interact with hosts and ecosystems. Traditional methods often miss low-abundance species and functional insights, leaving much of the microbiome unexplored. uMetaP, a revolutionary tool combining cutting-edge techniques including DIA-PASEF and Stable Isotope Labeling in Cell Culture (SILAC) enabled detection of microbial proteins at a sensitivity up to 5,000 times greater than previous methods, offering detailed taxonomic and functional profiling.  In just 30 minutes, uMetaP identified 47,925 protein groups, 220 microbial species, and 223 KEGG pathways with remarkable accuracy and reproducibility. It revealed previously undetectable proteins, including small proteins, proteins of unknown function, and potential new antibiotics. By detecting bacterial species representing as little as 0.001% of total biomass, uMetaP paves the way for early disease diagnosis and deeper insights into microbial diversity. This revolutionary platform sets a new standard in microbiome research, empowering scientists to explore microbial ecosystems with unparalleled depth and precision—truly a gift for science this holiday season. The NanoPhotometer N60 was used to measure the peptide concentration. Visit www.implen.de to find out how the NanoPhotometer can improve your research. Author: Feng Xian, Malena Brenek, David Gómez-Varela, Christoph Krisp, Ranjith Kumar Ravi Kumar, Manuela Schmidt #NanoPhotometer #JournalClub