Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

R. G. Barlow; D. G. Cummings; S. W. Gibb

doi:10.3354/meps161303

MEPS

Marine Ecology Progress Series

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MEPS 161:303-307 (1997) - doi:10.3354/meps161303

Improved resolution of mono- and divinyl chlorophylls a and b and zeaxanthin and lutein in phytoplankton extracts using reverse phase C-8 HPLC

R. G. Barlow*, D. G. Cummings, S. W. Gibb

Plymouth Marine Laboratory, Prospect Place, West Hoe, Plymouth PL1 3DH, United Kingdom

*Present address: Sea Fisheries Research Institute, Private Bag X2, Rogge Bay 8012, Cape Town, South Africa. E-mail: rgbarlow@sfri.wcape.gov.za

A reverse phase HPLC (high performance liquid chromatography) technique using a binary solvent system with a linear gradient on a Hypersil^® MOS2 C-8 column is described. As well as the resolution of key chemotaxonomic chlorophyll and carotenoid pigments, baseline separation of mono- and divinyl chlorophyll a and of lutein and zeaxanthin, and partial separation of mono- and divinyl chlorophyll b are achieved in a total analysis time of less than 30 min. The method provides an optimal balance between analyte resolution and sample throughput and is hence well suited to the analysis of oceanographic samples.

HPLC · C-8 column · Improved resolution · Chlorophylls · Carotenoids

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