Cultiply | Intelligent fermentation

𝐏𝐨𝐬𝐭 𝐍º1 of the series “Protein expression: from microbial strains to large-scale manufacturing”. 𝐇𝐨𝐰 𝐭𝐨 𝐜𝐡𝐨𝐨𝐬𝐞 𝐭𝐡𝐞 𝐫𝐢𝐠𝐡𝐭 𝐦𝐢𝐜𝐫𝐨𝐛𝐢𝐚𝐥 𝐬𝐭𝐫𝐚𝐢𝐧 𝐟𝐨𝐫 𝐩𝐫𝐨𝐭𝐞𝐢𝐧 𝐞𝐱𝐩𝐫𝐞𝐬𝐬𝐢𝐨𝐧. Selecting the right microbial strain is critical for optimizing protein expression. (Get it wrong, and you risk low yields or poor protein quality.) In our latest carousel, we break down the key factors to consider when choosing a strain. At Cultiply, we help you make the right choice for your specific needs. If you found this post insightful, like and share it! ______ Cultiply | Intelligent fermentation is a leading CDMO specializing in developing and manufacturing microbial solutions, guiding projects from concept to full-scale production. #ProteinExpression #Biotechnology #ScalingUp #Bioprocessing #Sustainability #CDMO

I’ve always been fascinated by how microbes influence plant health. But what if I told you they could also predict how well a crop will grow? A recent study in *Nature Microbiology* suggests we might be getting closer to making this a reality. The scale of the study is impressive: researchers analyzed the microbiomes of seed potatoes from 240 fields, sequencing microbial DNA from tuber. They then tracked plant growth using drones and built a machine-learning model to link microbial composition with potato vigor (the ability of seed tubers to sprout and grow into strong, productive plants.) What stood out to me: 👉 AI showed promise in predicting plant growth based on microbial data, but accuracy varied depending on the potato variety. 👉 Some microbes could be strong predictors of crop health. For example, *Streptomyces* can boost plant vigor by fighting off pathogens, while others like *Acinetobacter* and *Cellvibrio* might slow down growth. I find it quite exciting to think that with more data, we could fine-tune these models to help design custom biostimulants, biopesticides, and even microbiome-informed breeding strategies. At Cultiply, this is exactly the kind of science-backed innovation we love: using microbial solutions to help crops thrive while reducing reliance on chemicals. Source: https://lnkd.in/djCQFP3S Interested in microbial fermentation solutions? Let’s connect! I enjoy sharing insights and discussing the field #Biotechnology #Agritech #SustainableFarming #CDMO #Sustainability

We’re starting a new series of posts on protein expression: 𝐟𝐫𝐨𝐦 𝐦𝐢𝐜𝐫𝐨𝐛𝐢𝐚𝐥 𝐬𝐭𝐫𝐚𝐢𝐧𝐬 𝐭𝐨 𝐥𝐚𝐫𝐠𝐞-𝐬𝐜𝐚𝐥𝐞 𝐦𝐚𝐧𝐮𝐟𝐚𝐜𝐭𝐮𝐫𝐢𝐧𝐠. This is a topic we’ve spent years working on, and we thought it could be helpful to share what we’ve learned along the way. Our plan is to walk through the process step by step, covering everything from selecting the right strain to scaling up production. It’s a challenging path, but one we believe can be made easier with the right insights. Here’s what we’ll explore: ▪️ Selecting the right microbial strain ▪️ Optimizing fermentation processes ▪️ Addressing scale-up challenges (lab to production) ▪️ Bridging upstream and downstream processes ▪️ Building sustainable, cost-effective systems We’ll also share: ➡ Practical tips ➡ Real-world examples ➡ Straightforward solutions 𝐓𝐡𝐞 𝐟𝐢𝐫𝐬𝐭 𝐩𝐨𝐬𝐭 𝐰𝐢𝐥𝐥 𝐛𝐞 𝐥𝐢𝐯𝐞 𝐨𝐧 𝐅𝐞𝐛𝐫𝐮𝐚𝐫𝐲 12. If you’d like to follow along, feel free to hit the 🔔 at the top of our page to stay updated. And if someone in your network might find this useful, please share it with them. We hope it’s helpful! _________ Cultiply | Intelligent fermentation is a leading CDMO specializing in developing and manufacturing microbial solutions, guiding projects from concept to full-scale production. #ProteinExpression #Biotechnology #ScalingUp #Bioprocessing #Sustainability #BiotechInnovation

🎄 Have you written your biotech holiday wish list? The holiday season is all about surprises and gifts and in biotech, we dream big! We’re curious: what’s on your wish list this year? #Biotechnology #CDMO #Biotech #Bioprocess #Bioreactor

🌎The climate crisis is the pivotal challenge of our time. Like many, I often wonder if the solutions we need are big enough to address its scale. A recent editorial published in Nature Microbiology and 14 other leading journals renewed my hope. Authored by a remarkable group of scientists, including Raquel Peixoto, Christian R. Voolstra, Lisa Y. Stein, Philip Hugenholtz, and Jack A. Gilbert, it calls for urgent action to deploy microbial solutions to combat climate change. The editorial is concise and impactful. If you’re interested, I’ve added the link in the comments. Here’s a brief summary: 🦠MICROBES: SMALL BUT POWERFUL ALLIES Microbes are critical to Earth’s systems, regulating greenhouse gases, storing carbon, and sustaining ecosystems. The editorial highlights several actionable microbial strategies: - Carbon sequestration: storing CO₂ in soils and oceans while improving soil productivity. - Methane oxidation: reducing methane emissions from landfills, livestock, and wetlands. - Bioenergy production: creating renewable biofuels from algae and microbes. - Bioremediation: breaking down pollutants to restore contaminated land and water. - Nitrogen management: Using microbes to reduce synthetic fertilizer use and greenhouse gas emissions. 🆘URGENT ACTION IS NEEDED The authors call for a globally coordinated response like what we saw during COVID-19. They propose a science-led task force to rapidly deploy microbial solutions, facilitate collaboration, and streamline regulations while ensuring safety. 🌈HOPE THROUGH ACTION What struck me most was the balance of urgency and optimism. While the challenges are immense, the solutions are tangible and within reach if we act now. This is a reminder that microbes can be key allies in tackling the climate crisis. Source: https://lnkd.in/dSECXbCG #ClimateAction #Microbiology #Sustainability #Innovation

Ever heard of Design of Experiments (DOE)? It’s a simple, practical way to save time and improve results in your work. At its core, 𝗗𝗢𝗘 𝗶𝘀 𝗮 𝘀𝗺𝗮𝗿𝘁𝗲𝗿 𝗮𝗽𝗽𝗿𝗼𝗮𝗰𝗵 𝘁𝗼 𝘁𝗲𝘀𝘁𝗶𝗻𝗴 𝗮𝗻𝗱 𝗽𝗿𝗼𝗯𝗹𝗲𝗺-𝘀𝗼𝗹𝘃𝗶𝗻𝗴. Instead of changing one thing at a time and guessing what works, DOE helps you test multiple factors together to determine: 1️⃣ What truly matters (e.g., temperature, pH, nutrients). 2️⃣ How different factors interact (like how pH might affect nutrient absorption). 3️⃣ The best combination to achieve your goals quickly and efficiently. 𝗛𝗲𝗿𝗲’𝘀 𝗮 𝗽𝗿𝗮𝗰𝘁𝗶𝗰𝗮𝗹 𝗲𝘅𝗮𝗺𝗽𝗹𝗲: A bioprocessing team wanted to optimize fermentation for an enzyme. They identified three key factors to test: ✔️ 𝗧𝗲𝗺𝗽𝗲𝗿𝗮𝘁𝘂𝗿𝗲: Needed to grow microbes without damaging the enzyme. ✔️ 𝗡𝘂𝘁𝗿𝗶𝗲𝗻𝘁𝘀: Too little stalled growth; too much increased costs. ✔️ 𝗠𝗶𝘅𝗶𝗻𝗴 𝘀𝗽𝗲𝗲𝗱: Too slow reduced oxygen transfer; too fast stressed the cells. Using DOE, they designed 9 experiments to test combinations of these factors. Instead of running 27 unique trials manually, they grouped tests strategically. The results? ➜ They pinpointed the ideal conditions that boosted enzyme yield by 40%. ➜ They saved both time and resources by testing efficiently. 𝗪𝗵𝘆 𝘀𝗵𝗼𝘂𝗹𝗱 𝘁𝗵𝗶𝘀 𝗺𝗮𝘁𝘁𝗲𝗿 𝘁𝗼 𝘆𝗼𝘂? If you’re working in areas like protein expression or fermentation, DOE can help you: 🔹 𝗦𝗮𝘃𝗲 𝘁𝗶𝗺𝗲 Identify what works faster. 🔹 𝗔𝘃𝗼𝗶𝗱 𝘄𝗮𝘀𝘁𝗲: Reduce trial-and-error experiments. 🔹 𝗠𝗮𝗸𝗲 𝗯𝗲𝘁𝘁𝗲𝗿 𝗱𝗲𝗰𝗶𝘀𝗶𝗼𝗻𝘀: Use data, not guesses, to scale confidently. At first, the principles of DOE might seem challenging to grasp but once you understand it, the advantages are undeniable. Curious how this could apply to your work? Let’s talk! Drop a comment or reach out directly at hola@cultiply.es. _ Cultiply | Intelligent fermentation is a leading CDMO specializing in developing and manufacturing microbial solutions, guiding projects from concept to full-scale production. #Biotechnology #DOE #Bioporcess #CDMO

Oxygen transfer: simple in the lab, but scaling up? ■ Larger volumes mean less surface area for gas exchange. ■ Too much stirring can harm delicate cells. ■ Poor mixing creates “dead zones” where oxygen doesn’t reach. Combined, these factors can make oxygen transfer a significant bottleneck when scaling production. So, how can you tackle these challenges effectively? Here are 4 practical tips to help you improve oxygen transfer as you scale: 1️⃣ Understand your system’s oxygen needs. Start with small-scale experiments to measure your cells’ oxygen demand and your system’s delivery efficiency. Metrics like kLa (oxygen transfer rate) and OUR (oxygen uptake rate) will be critical to guiding your scaling process. 2️⃣ Optimize mixing and sparging. Not all stirrer designs or sparging systems are created equal. Test various setups to ensure oxygen is evenly distributed while minimizing stress on sensitive cells. 3️⃣ Continuously monitor oxygen levels. Use real-time sensors in your bioreactor to detect oxygen-depleted zones early. This allows you to adjust parameters quickly, preventing production issues. 4️⃣ Scale up in stages. Pilot runs are your best friend. They help you refine the process, troubleshoot issues, and build confidence before moving to full-scale production. The takeaway: Scaling is complex, but by addressing oxygen transfer challenges with these strategies, you can achieve reliable and consistent results. If you found these tips helpful, share them with your network to help others succeed! _ Cultiply is a leading CDMO specializing in developing and manufacturing microbial solutions, guiding projects from concept to full-scale production. #Biotechnology #CDMO #Bioreactor #Bioprocess

Scaling production is a crucial step in biotech growth. The question is: Should you scale bigger or replicate smaller? This carousel explores: ✔️ Key differences between the strategies ✔️ Pros and cons of each approach ✔️ Tips to choose the best path for your needs If you need help to choose or scale, contact us ! hola@cultiply.es _ Cultiply is a leading CDMO specializing in developing and manufacturing microbial solutions, guiding projects from concept to full-scale production. #Biotechnology #CDMO #Scaleup #Bioprocess #Biomanufacturing

Innovation starts in the lab, but it grows in the real world. One thing I’ve noticed in biotechnology is how often great ideas struggle to leave the lab. We focus so much on the science, but turning a discovery into something scalable and impactful is a completely different challenge. Here’s the reality: A lab breakthrough is just the beginning. Scaling it to production requires new skills, strategies, and resources. Without proper planning, even the best science can stall. Think about it: ❌ A brilliant process that works at 10L might fail completely at 10,000L. ❌ A small inefficiency in the lab can balloon into a costly bottleneck in production. Scaling isn’t just about size. It’s about consistency, foresight, perseverance, and long-term impact. And that’s where we need to focus more attention. Biotechnology has the power to transform industries and improve lives. But if we want these innovations to succeed, we have to ask: Are we designing with the end goal in mind? Are we building processes that scale efficiently? Are we ready to address challenges early, before they grow? These are the questions that keep me motivated every day. Innovation doesn’t end in the lab. It starts there.

Innovation does not happen in isolation. It thrives on collaboration! That’s why we’re thrilled to announce our partnership with the MAI CDMO Marketplace. 𝐖𝐡𝐚𝐭 𝐢𝐬 𝐭𝐡𝐞 𝐌𝐀𝐈 𝐂𝐃𝐌𝐎 𝐌𝐚𝐫𝐤𝐞𝐭𝐩𝐥𝐚𝐜𝐞? It’s a platform that connects #biopharma organizations with trusted partners like Cultiply to develop and manufacture their products. Turning ideas into reality, from concept to full-scale production 𝐖𝐡𝐚𝐭 𝐝𝐨𝐞𝐬 𝐭𝐡𝐢𝐬 𝐦𝐞𝐚𝐧 𝐟𝐨𝐫 𝐮𝐬 (𝐚𝐧𝐝 𝐟𝐨𝐫 𝐲𝐨𝐮)? Joining MAI empowers us to: > Expand opportunities: Connecting with innovators seeking customized manufacturing solutions. > Foster collaboration: Partnering with industry leaders to drive breakthroughs and accelerate progress. > Maximize impact: Helping our partners bring biotech advancements to market with precision and speed. It’s an opportunity to build something greater, together! #CDMO #Biotechnology #Biomanufacturing #Bioprocess #Collaboration