52008PC0715

[pic] | COMMISSION OF THE EUROPEAN COMMUNITIES |

Brussels, 21.11.2008

COM(2008) 715 final

2008/0219 (CNS)

Proposal for a

COUNCIL DIRECTIVE

on animal health conditions governing the movement and importation from third countries of equidae (Codified version)

(presented by the Commission)

EXPLANATORY MEMORANDUM

1. In the context of a people’s Europe, the Commission attaches great importance to simplifying and clarifying Community law so as to make it clearer and more accessible to the ordinary citizen, thus giving him new opportunities and the chance to make use of the specific rights it gives him.

This aim cannot be achieved so long as numerous provisions that have been amended several times, often quite substantially, remain scattered, so that they must be sought partly in the original instrument and partly in later amending ones. Considerable research work, comparing many different instruments, is thus needed to identify the current rules.

For this reason a codification of rules that have frequently been amended is also essential if Community law is to be clear and transparent.

2. On 1 April 1987 the Commission therefore decided[1] to instruct its staff that all legislative acts should be codified after no more than ten amendments, stressing that this is a minimum requirement and that departments should endeavour to codify at even shorter intervals the texts for which they are responsible, to ensure that the Community rules are clear and readily understandable.

3. The Conclusions of the Presidency of the Edinburgh European Council (December 1992) confirmed this[2], stressing the importance of codification as it offers certainty as to the law applicable to a given matter at a given time.

Codification must be undertaken in full compliance with the normal Community legislative procedure.

Given that no changes of substance may be made to the instruments affected by codification , the European Parliament, the Council and the Commission have agreed, by an interinstitutional agreement dated 20 December 1994, that an accelerated procedure may be used for the fast-track adoption of codification instruments.

4. The purpose of this proposal is to undertake a codification of Council Directive 90/426/EEC of 26 June 1990 on animal health conditions governing the movement and import from third countries of equidae[3]. The new Directive will supersede the various acts incorporated in it[4]; this proposal fully preserves the content of the acts being codified and hence does no more than bringing them together with only such formal amendments as are required by the codification exercise itself.

5. The codification proposal was drawn up on the basis of a preliminary consolidation , in all official languages, of Directive 90/426/EEC and the instruments amending it, carried out by the Office for Official Publications of the European Communities, by means of a data-processing system . Where the Articles have been given new numbers, the correlation between the old and the new numbers is shown in a table contained in Annex VI to the codified Directive.

ê 90/426/EEC (adapted)

2008/0219 (CNS)

Proposal for a

COUNCIL DIRECTIVE

on animal health conditions governing the movement and Ö importation Õ from third countries of equidae (codified version)

(Text with EEA relevance)

THE COUNCIL OF THE EUROPEAN UNION,

Having regard to the Treaty establishing the European Community, and in particular Article Ö 37 Õ thereof,

Having regard to the proposal from the Commission,

Having regard to the opinion of the European Parliament[5],

Whereas:

ê

(1) Council Directive 90/426/EEC of 26 June 1990 on animal health conditions governing the movement and import from third countries of equidae[6] has been substantially amended several times[7]. In the interests of clarity and rationality the said Directive should be codified.

ê 90/426/EEC Recital 1 (adapted)

(2) Equidae, being live animals, are included in the list of products in Annex Ö I Õ to the Treaty.

ê 90/426/EEC Recital 2 (adapted)

(3) In order to ensure the rational development of equidae production, thereby increasing productivity in that sector, rules governing the movement of equidae between Member States Ö should Õ be laid down at Community level.

ê 90/426/EEC Recital 3

(4) The breeding and rearing of equidae and in particular of horses is generally included in the farming sector. It constitutes a source of income for part of the farming population.

ê 90/426/EEC Recital 4

(5) Disparities as regards animal health conditions in the Member States should be eliminated in order to encourage intra-Community trade in equidae.

ê 90/426/EEC Recital 5 (adapted)

(6) In order to encourage the harmonious development of intra-Community trade, a Community system should be Ö provided for Õ to govern imports from third countries.

ê 90/426/EEC Recital 6

(7) The conditions for the movement on national territory of equidae bearing an identification document should also be regulated.

ê 90/426/EEC Recital 7 (adapted)

(8) In order to be the subject of trade, equidae Ö should Õ satisfy certain animal health requirements, so as to avoid the spreading of Ö infectious or Õ contagious diseases. It appears in particular appropriate to provide for a possible regionalisation of restrictive measures.

ê 90/426/EEC Recital 8 (adapted)

(9) The transport conditions should be laid down for the same reasons Ö taking into account the animal welfare conditions laid down in Council Regulation (EC) No 1/2005[8] Õ.

ê 90/426/EEC Recital 9 (adapted)

(10) To ensure that those requirements are satisfied provision Ö should Õ be made for the issue by an official veterinarian of a health certificate to accompany the equidae to their place of destination.

ê 90/426/EEC Recital 10 (adapted)

(11) The organisation of and the follow-up to the checks to be carried out by the Member State of destination and the safeguard measures to be implemented Ö have been laid down in Council Directive 90/425/EEC of 26 June 1990 concerning veterinary and zootechnical checks applicable in intra-Community trade in certain live animals and products with a view to the completion of the internal market[9] Õ.

ê 90/426/EEC Recital 11

(12) Provision should be made for the possibility of checks by the Commission. These checks should be carried out in cooperation with the competent national authorities.

ê 90/426/EEC Recital 12

(13) Defining Community provisions applicable to imports from third countries requires a list to be drawn up of third countries or parts of third countries from which equidae may be imported.

ê 90/426/EEC Recital 13 (adapted)

(14) The choice of those countries Ö should Õ be based on criteria of a general nature such as the state of health of the livestock, the organisation and powers of the veterinary services and the health regulations in force.

ê 90/426/EEC Recital 14

(15) In addition, imports of equidae should not be authorised from countries infected with infectious or contagious animal diseases which present a risk to Community livestock or which have been free from such infection for too short a period. Such considerations are also valid for imports from third countries in which vaccination against such diseases is carried out.

ê 90/426/EEC Recital 15 (adapted)

(16) The general conditions applicable to imports from third countries Ö should Õ be supplemented by special conditions drawn up on the basis of the health situation in each of them. The technical nature and the diversity of the criteria on which those special conditions depend require for their definition recourse to a flexible and rapid Community procedure in which the Commission and the Member States cooperate closely.

ê 90/426/EEC Recital 16 (adapted)

(17) The presentation of a common standard form of certificate upon import of equidae constitutes an effective means of verifying that the Community rules are being applied. Such rules may include special provisions which may vary according to the third country concerned, and this Ö should Õ be taken into account in drawing up the standard forms of certificates.

(18) ê 90/426/EEC Recital 17 (adapted)

(19) Ö Veterinary experts of the Commission and of the Member States, appointed by the Commission, Õ should be responsible for verifying that the requirements of this Directive are observed, particularly in third countries.

ê 90/426/EEC Recital 18 (adapted)

(20) The checks carried out upon importation Ö should Õ cover the origin and the state of health of the equidae.

ê 90/426/EEC Recital 22 (adapted)

(21) Ö The measures necessary for the implementation of this Directive should be adopted in accordance with Council Decision 1999/468/EC of 28 June 1999 laying down the procedures for the exercise of implementing powers conferred on the Commission[10]. Õ

ê

(22) This Directive should be without prejudice to the obligations of the Member States relating to the time-limits for transposition into national law of the Directives set out in Annex V, Part B,

ê 90/426/EEC

HAS ADOPTED THIS DIRECTIVE:

CHAPTER I

General provisions

Article 1

ê 90/426/EEC (adapted)

This Directive lays down animal health conditions for the movement Ö within and Õ between Member States and Ö importation Õ from third countries of live equidae.

Article 2

For the purposes of this Directive Ö the following definitions shall apply Õ:

ê 90/426/EEC

(a) ‘holding’ means an agricultural or training establishment, a stable or, generally speaking, any premises or facilities in which equidae are habitually kept or bred, for whatever use;

(b) ‘equidae’ means wild or domesticated animals of the equine (including zebras) or asinine species or the offspring of crossings of those species;

(c) ‘registered equidae’ means any equidae registered as defined in Council Directive 90/427/EEC[11], identified by means of an identification document issued:

(i) by the breeding authority or any other competent authority of the country where the animal originated which manages the studbook or register for that breed of animal; or

ê 90/426/EEC (adapted)

(ii) Ö by Õ any international association or organisation which manages horses for competition or racing;

(d) ‘equidae for slaughter’ means equidae intended to be transported either directly or after transit through an approved marshalling centre Ö , referred to in Article 7, Õ to the slaughterhouse for slaughter;

ê 90/426/EEC

(e) ‘equidae for breeding and production’ means equidae other than those mentioned in (c) and (d);

(f) ‘Member State or third country free from African horse sickness’ means any Member State or third country in which there has been no clinical, serological (in unvaccinated equidae) or epidemiological evidence of African horse sickness on the territory concerned in the previous two years and in which there have been no vaccinations against the disease during the previous 12 months;

(g) ‘compulsorily notifiable diseases’ means the diseases listed in Annex I;

(h) ‘official veterinarian’ means the veterinarian designated by the competent central authority of a Member State or of a third country;

ê 90/426/EEC (adapted)

(i) ‘temporary admission’ means the status of registered Ö equidae Õ originating in a third country and admitted into Community territory for a period of less than 90 days to be fixed in accordance with the procedure Ö referred to Õ in Article 21(2), depending on the health situation in the country of origin.

CHAPTER II

Rules for the movement of equidae Ö within and between Member States Õ

ê 90/426/EEC

Article 3

Member States shall authorise the movement of equidae registered in their territory or send equidae to another Member State only where they satisfy the conditions laid down in Articles 4 and 5.

However, the competent authorities in Member States of destination may grant general or limited exemption in respect of movement of equidae which:

- are being ridden or taken, for sporting or recreational purposes, along roads situated near internal borders of the Community,

- are taking part in cultural or similar events or in activities organised by authorised local bodies situated near internal borders of the Community,

- are intended solely for temporary pasturing or work near internal borders of the Community.

Member States making use of such authorisation shall inform the Commission of the content of the exemptions granted.

Article 4

1. Equidae must show no clinical sign of disease at inspection. Inspection must be carried out in the 48 hours prior to their embarkation or loading. In the case of registered equidae, however, this inspection shall, without prejudice to Article 6, be required for intra-Community trade only.

2. Without prejudice to the requirements of paragraph 5 regarding compulsorily notifiable diseases, the official veterinarian must, at the time of inspection, be satisfied that there are no grounds — in particular on the basis of declarations by the owner or breeder — for concluding that the equidae have been in contact with equidae suffering from an infectious or contagious disease during the 15 days immediately preceding inspection.

3. The equidae must not be intended for slaughter under a national programme of infectious or contagious disease eradication.

4. The equidae must be identified in the following manner:

ê 90/426/EEC (adapted)

(a) in the case of registered Ö equidae Õ , by means of an identification document, as provided for in Directive 90/427/EEC, which must certify in particular that Ö paragraphs 5 and 6 of this Article and Õ Article 5 of this Directive have been complied with.

The official veterinarian must suspend the validity of Ö the identification Õ document for the period of the prohibitions provided for in paragraph 5 Ö of this Article Õ or in Article 5 of this Directive. The Ö identification Õ document must, following the slaughter of the registered horse, be returned to the authority which issued it. The procedure for the implementation of this point shall be adopted in accordance with the procedure Ö referred to Õ in Article 21(2);

(b) for equidae for breeding and production, by Ö the Õ method established in accordance with the procedure Ö referred to Õ in Article 21(2).

ê 90/426/EEC

5. In addition to the requirements laid down in Article 5, the equidae must not come from a holding which has been the subject of one of the following prohibition orders:

(a) if all the animals of species susceptible to the disease located on the holding have not been slaughtered, the period of prohibition concerning the holding of origin must be at least:

(i) six months in the case of equidae suspected of having contracted dourine, beginning on the date of the last actual or possible contact with a sick animal. However, in the case of a stallion, the prohibition shall apply until the animal is castrated;

(ii) six months in the case of glanders or equine encephalomyelitis, beginning on the day on which the equidae suffering from the disease in question are slaughtered;

(iii) in the case of infectious anaemia, until the date on which, the infected animals having been slaughtered, the remaining animals have shown a negative reaction to two Coggins tests carried out three months apart;

(iv) six months in the case of vesicular stomatitis;

(v) one month from the last recorded case, in the case of rabies;

(vi) 15 days from the last recorded case, in the case of anthrax;

(b) if all the animals of species susceptible to the disease located on the holding have been slaughtered and the premises disinfected, the period of prohibition shall be 30 days, beginning on the day on which the animals were destroyed and the premises disinfected, except in the case of anthrax, where the period of prohibition is 15 days.

ê 90/426/EEC (adapted)

The competent authorities may derogate from these prohibition Ö orders Õ for hippodromes and racecourses, and shall notify the Commission of the nature of any derogations granted.

6. Where a Member State draws up or has drawn up a voluntary or compulsory control programme for a disease to which equidae are susceptible, it may present the programme to the Commission, outlining in particular:

ê 90/426/EEC

(a) the distribution of the disease on its territory;

(b) the reasons for the programme, taking into consideration the significance of the disease and its cost/benefit advantages;

(c) the geographical area in which the programme will be implemented;

(d) the status categories to be applied to establishments, the standards which must be attained for each species and the test procedures to be used;

(e) the programme monitoring procedures;

(f) the action to be taken if, for any reason, a holding loses its status;

(g) the measures to be taken if the results of the tests carried out in accordance with the provisions of the programme are positive;

(h) the non-discriminatory nature of trade in the territory of the Member State concerned with respect to intra-Community trade.

ê 90/426/EEC (adapted)

The Commission shall examine the programmes presented by the Member States. Where appropriate it shall approve them in accordance with the procedure Ö referred to Õ in Article 21(2). Any additional guarantees, general or specific, which may be required in intra-Community trade may be defined in accordance with the same procedure. Such guarantees must not exceed those required by the Member State in its own territory.

Programmes submitted by Member States may be amended or supplemented in accordance with the procedure Ö referred to Õ in Article 21(3). Amendments or additions to programmes which have already been approved or to guarantees which have been defined in accordance with the second subparagraph may be approved under the same procedure.

ê 92/36/EEC Art. 1

Article 5

1. A Member State which is not free from African horse sickness within the meaning of Article 2(f) may dispatch equidae from that part of its territory which is considered to be infected within the meaning of paragraph 2 of this Article only under the conditions set out in paragraph 5 of this Article.

2. A part of the territory of a Member State shall be considered to be infected with African horse sickness if:

(a) clinical, serological (in unvaccinated animals) and/or epidemiological evidence has revealed the presence of African horse sickness in the past two years; or

(b) vaccination against African horse sickness has been carried out in the past 12 months.

ê 92/36/EEC Art. 1 (adapted)

The part of the territory considered to be infected with African horse sickness Ö shall Õ comprise as a minimum:

ê 92/36/EEC Art. 1

(a) a protection zone with a radius of at least 100 km around any centre of infection;

(b) a surveillance zone at least 50 km extending beyond the protection zone, in which no vaccination has been carried out in the last 12 months.

ê 92/36/EEC Art. 1 (adapted)

3. The Ö control Õ rules Ö and Õ the Ö measures to Õ combat Ö African horse sickness Õ relating to the territories and zones referred to in paragraph 2 and the relevant derogations are specified in Council Directive [92/35/EEC[12]].

4. All vaccinated equidae found in the protection zone must be registered and Ö marked Õ in accordance with Article [6(1)(d)] of Directive [92/35/EEC].

ê 92/36/EEC Art. 1

The identification document and/or health certificate shall carry a clear reference to such vaccination.

5. A Member State may dispatch from the territory referred to in the second subparagraph of paragraph 2 only equidae which meet the following requirements:

ê 92/36/EEC Art. 1 (adapted)

(a) they must be dispatched only during certain periods of the year, having regard to the activity of vector insects, to be determined in accordance with the procedure Ö referred to Õ in Article 21(3);

(b) they must show no clinical symptom of African horse sickness on the day of the inspection referred to in Article 4(1);

(c) they must have undergone a test for African horse sickness as described in Annex IV, on two occasions, with an interval of between 21 and 30 days between the two tests, the second of which must have been carried out during the 10 days prior to dispatch:

(i) either with negative results, if they have not been vaccinated against African horse sickness; or

(ii) without having recorded an increase in the antibody count and without having undergone vaccination during the previous two months, if they have been vaccinated against African horse sickness.

Ö In accordance with Õ the procedure Ö referred to Õ in Article 21(2), , and following the opinion of the Ö European Food Safety Authority Õ , other monitoring methods may be recognised;

ê 92/36/EEC Art. 1

(d) they must have been kept in a quarantine station for a minimum period of 40 days prior to dispatch;

(e) they must have been protected from vector insects during the period of quarantine and during transportation from the quarantine station to the place of dispatch.

ê 90/426/EEC (adapted)

Article 6

Member States which implement an alternative control system providing guarantees equivalent to those laid down in Article 4(5) as regards movements within their territory of equidae may grant one another derogations from the provisions of the second sentence of Article 4(1) and Article 8(1)(b) on a reciprocal basis.

They shall notify the Commission thereof.

Article 7

ê 2008/73/EC Art. 7 (adapted)

1. Equidae must be transported, as soon as possible, from the holding of origin either directly or via an approved marshalling centre, as defined as “assembly centre” in Article 2(2)(o) of Council Directive 64/432/EEC[13], to the place of destination in vehicles or containers which have been regularly cleansed and disinfected with a disinfectant at intervals to be fixed by the Member State of dispatch. The vehicles must be designed in such a way that equidae droppings, litter or fodder cannot escape from the vehicle during transportation. Ö Without prejudice to Regulation (EC) No 1/2005, Õ transportation must be effected in such a way that the health and well-being of the equidae can be protected effectively.

ê 90/426/EEC (adapted)

2. The Member State of destination may, on a general or restricted basis, grant a derogation from some of the requirements of Article 4(5) for any animal bearing a special mark indicating that it is scheduled for slaughter, provided that the health certificate Ö in accordance with Annex III Õ mentions such derogation.

ê 90/426/EEC

Where such a derogation is granted, equidae for slaughter must be transported directly to the designated slaughterhouse and be slaughtered within five days of arrival at the slaughterhouse.

3. The official veterinarian must record the identification number or identification document number of the slaughtered animal and forward to the competent authority of the place of dispatch, at the latter's request, an attestation to the effect that the animal has been slaughtered.

ê 90/426/EEC (adapted)

Article 8

1. Member States shall ensure that:

(a) registered equidae which leave their holdings are accompanied by the identification document laid down in Article 4(4)(a) together, if they are intended for intra-Community trade, with the Ö health Õ attestation provided for in Annex II;

(b) equidae for breeding, production and slaughter are, during their transportation, accompanied by a health certificate complying with Annex III .

2. The Ö health Õ certificate, or in the case of Ö registered equidae the health attestation, Õ must, without prejudice to Article 6, be drawn up during the 48 hours preceding their embarkation or else no later than the last working day prior to it, in at least one of the official languages of the Member States of dispatch and destination. The duration of validity of the Ö health Õ certificate Ö or health attestation Õ shall be 10 days. The Ö health Õ certificate Ö or health attestation Õ must consist of a single sheet.

3. Ö For the movement between Member States, Õ equidae other than registered equidae may be covered by a single health certificate per consignment rather than by the individual Ö health Õ certificate referred to in paragraph 1, point b).

ê 90/425/EEC Art. 15(3)

Article 9

The rules laid down in Directive 90/425/EEC , shall apply in particular to checks at origin, to the organisation of, and follow-up to, the checks to be carried out by the Member State of destination, and to the safeguard measures to be implemented.

ê 90/426/EEC

Article 10

Veterinary experts from the Commission may, to the extent necessary to ensure uniform application of this Directive and in cooperation with the competent national authorities, carry out on-the-spot inspections. The Commission shall inform the Member States of the outcome of such inspections.

The Member States in whose territory an inspection is carried out shall give the experts all the assistance necessary to carry out their task.

ê 90/426/EEC (adapted)

General arrangements for the application of this Article shall be adopted in accordance with the procedure Ö referred to Õ in Article 21(2).

CHAPTER III

Rules for Ö importation of equidae Õ from third countries

Article 11

Equidae imported into the Community must satisfy the conditions laid down in Articles 12 to 16.

ê 2004/68/EC Art. 15(1) (adapted)

Article 12

1. The importation of equidae into the Community shall only be authorised from third countries that appear on a list to be drawn up or amended in accordance with the procedure referred to in Article 21(2).

ê 2004/68/EC Art. 15(1)

Taking into account the health situation and the guarantees provided by the third country for equidae, it may be decided in accordance with the procedure referred to in Article 21(2) that the authorisation provided for in the first subparagraph of this paragraph shall apply to the whole territory of the third country or to only part of its territory.

For that purpose and on the basis of the relevant international standards, account shall be taken of how the third country applies and implements those standards, in particular the principle of regionalisation, within its own territory and in relation to its sanitary requirements for importation from other third countries and from the Community.

ê 2004/68/EC Art. 15(1) (adapted)

2. When the list provided for in paragraph 1 Ö is Õ drawn up or amended, particular account shall be taken of:

ê 2004/68/EC Art. 15(1)

(a) the health status of the equidae, other domestic animals and wildlife in the third country, with particular regard to exotic animal diseases and any aspects of the general health and the environmental situation in the third country which may pose a risk to the health and environmental status of the Community;

(b) the legislation of the third country in relation to animal health and welfare;

(c) the organisation of the competent veterinary authority and its inspection services, the powers of those services, the supervision to which they are subject, and the means at their disposal, including staff and laboratory capacity, to apply national legislation effectively;

(d) the assurances which the competent veterinary authority of the third country can give regarding compliance or equivalence with the relevant animal health conditions applicable in the Community;

ê 2004/68/EC Art. 15(1) (adapted)

(e) whether the third country is a member of the Ö World Organisation for Animal Health Õ (OIE) and the regularity and rapidity of the information supplied by the third country relating to the existence of infectious or contagious diseases of equidae in its territory, in particular those diseases listed by the OIE and in Annex I ;

ê 2004/68/EC Art. 15(1)

(f) the guarantees given by the third country to directly inform the Commission and the Member States:

(i) within 24 hours, of the confirmation of the occurrence of infectious diseases of equidae listed in Annex I and of any change in the vaccination policy concerning such diseases;

(ii) within an appropriate period, of any proposed changes in the national sanitary rules concerning equidae, in particular regarding the importation of equidae;

(iii) at regular intervals, of the animal health status of its territory concerning equidae;

(g) any experience of previous imports of live equidae from the third country and the results of any import controls carried out;

(h) the results of Community inspections and/or audits carried out in the third country, in particular the results of the assessment of the competent authorities or, where the Commission so requests, the report submitted by the competent authorities on the inspections which they have carried out;

(i) the rules on the prevention and control of infectious or contagious animal diseases in force in the third country and their implementation, including rules on importation of equidae from other third countries.

ê 2004/68/EC Art. 15(1) (adapted)

3. The Commission shall arrange for up-to-date versions of the list drawn up or amended as provided for in paragraph 1 to be made available to the public.

The list may be combined with other lists drawn up for animal and public health purposes and may also include models of health certificates.

ê 2004/68/EC Art. 15(1)

4. Special import conditions for each third country or group of third countries, having regard to the animal health situation concerning equidae in the third country or countries concerned shall be established in accordance with the procedure referred to in Article 21(2).

ê 2004/68/EC Art. 15(1) (adapted)

5. Detailed rules for the application of paragraphs 1 to 4 and criteria for including third countries or parts of third countries in the list provided for in paragraph 1 may be adopted in accordance with the procedure referred to in Article 21(2).

ê 90/426/EEC

Article 13

1. The equidae must come from third countries which:

(a) are free from African horse sickness;

(b) have been free for two years from Venezuelan equine encephalomyelitis (VEE);

(c) have been free for six months from dourine and glanders.

ê 90/426/EEC (adapted)

2. In accordance with the procedure Ö referred to Õ in Article 21(2) it may be decided:

ê 90/426/EEC

(a) that the provisions of paragraph 1 of this Article shall apply to only a part of the territory of a third country.

In the event that the African horse sickness requirements apply on a regional basis, at the very least the measures laid down in Article 5(2) and (3) must be complied with;

(b) to require additional guarantees for diseases alien to the Community.

Article 14

Before the day of loading for transportation to the Member State of destination, the equidae must have remained without interruption in the territory or part of the territory of a third country or, in the event of regionalisation, in the part of the territory defined pursuant to Article 13(2)(a) for a period to be determined in the decisions to be adopted pursuant to Article 15.

They must come from a holding placed under veterinary supervision.

Article 15

Importation of equidae from the territory of a third country or part thereof as defined in accordance with Article 13(2)(a) on the list drawn up in accordance with Article 12(1) shall be authorised only if the equidae, over and above the requirements of Article 13:

ê 90/426/EEC (adapted)

(a) comply with the animal health requirements adopted, with reference to the species in question, the categories of equidae Ö and the type of importation Õ, in accordance with the procedure Ö referred to Õ in Article 21(2) for Ö importation Õ of equidae from that country.

The reference basis for fixing Ö those Õ animal health Ö requirements Õ shall be the standards laid down in Articles 4 and 5; and

ê 90/426/EEC

(b) in the case of a third country not free of vesicular stomatitis or viral arteritis for at least six months, the equidae must meet the following requirements:

(i) they must come from a holding which has been free of vesicular stomatitis for at least six months and they must have reacted negatively to a serological test prior to dispatch;

ê 90/426/EEC (adapted)

(ii) in the case of viral arteritis, male equidae must, notwithstanding Article 19(b), have reacted negatively to a serological test or to a virus isolation test or to any other test recognised in accordance with the procedure Ö referred to Õ in Article 21(2) which would guarantee freedom from the virus.

In accordance with the procedure Ö referred to Õ in Article 21(2), and following the opinion of the Ö European Food Safety Authority Õ, the categories of male equidae to which this requirement shall apply may be defined.

Article 16

1. The equidae must be identified in accordance with Article 4(4) and accompanied by a Ö health Õ certificate drawn up by an official veterinarian of the exporting third country. This Ö health Õ certificate must:

(a) be issued on the day of loading of the animals for dispatch to the Member State of destination or, in the case of registered horses, on the last working day before embarkation;

(b) be drawn up in at least one of the official languages of the Member State of destination and one of those of the Member State in which the import inspection is carried out;

(c) accompany the animals in the original;

(d) attest that the animals satisfy the requirements of this Directive and those laid down pursuant to this Directive with regard to importation from third countries;

(e) consist of a single sheet;

(f) be made out for a single consignee or, in the case of animals for slaughter, for a consignment, provided the animals are properly marked and identified.

2. The Ö health Õ certificate must be drawn up on a form complying with a model established in accordance with the procedure Ö referred to Õ in Article 21(2).

ê 90/426/EEC Article 18 (adapted)

Article 17

1. Immediately upon arrival in the Member State of destination, equidae for slaughter shall be taken to a slaughterhouse, either directly or after transition through an approved marshalling centre Ö , referred to in Article 7, Õ and, in accordance with animal health requirements, be slaughtered within a period specified in the decisions to be adopted pursuant to Article 15.

2. Without prejudice to any special conditions which may be adopted in accordance with the procedure Ö referred to Õ in Article 21(2), the competent authority of the Member State of destination may, on animal health grounds, designate the slaughterhouse to which such equidae must be taken.

ê 90/426/EEC Article 17

Article 18

Checks shall be carried out on the spot by veterinary experts of the Member States and the Commission to verify whether the provisions of this Directive, and in particular those of Article 12(2), are being applied in practice.

ê 90/426/EEC Article 17 (adapted)

Should checks carried out within the terms of this Article bring to light serious facts as against an approved holding, the Commission shall immediately inform the Member States and forthwith adopt a decision provisionally suspending the approval. The final decision shall be taken in accordance with the procedure Ö referred to Õ in Article 21(3).

ê 90/426/EEC Article 17

The experts from the Member States who are to be entrusted with those checks shall be appointed by the Commission, acting on a proposal from the Member States.

Those checks shall be made on behalf of the Community, which shall bear the cost of any expenditure incurred in this connection.

ê 90/426/EEC Article 17 (adapted)

The frequency of and the procedure for those checks shall be determined in accordance with the procedure Ö referred to Õ in Article 21(2).

ê 90/426/EEC (adapted)

Article 19

In accordance with the procedure Ö referred to Õ in Article 21(2):

(a) it may be decided that Ö importation Õ from a third country or part of a third country is to be confined to particular species or categories Ö of equidae or types of importation Õ;

ê 90/426/EEC

(b) notwithstanding Article 15, the special conditions for the temporary entry into Community territory of registered equidae or equidae intended for special uses or their re-entry into Community territory after being temporarily exported, shall be established;

(c) the conditions for converting temporary entry into permanent entry shall be determined;

ê 2004/68/EC Art. 15(2) (adapted)

(d) a Community reference laboratory for one or more of the diseases of equidae listed in Annex I may be designated and the functions, tasks and procedures regarding collaboration with laboratories responsible for diagnosing infectious diseases of equidae in the Member States Ö shall be provided for Õ.

ê 90/426/EEC (adapted)

CHAPTER IV

Final provisions

Article 20

Annexes I to IV shall be amended in accordance with the procedure Ö referred to Õ in Article 21(3).

ê 806/2003 Art. 3 and Annex III(10) (adapted)

Article 21

1. The Commission shall be assisted by the Standing Committee on the Food Chain and Animal Health set up pursuant to Article 58 of Regulation (EC) No 178/2002 of the European Parliament and of the Council[14].

2. Where reference is made to this Ö paragraph Õ, Articles 5 and 7 of Decision 1999/468/EC shall apply.

The period laid down in Article 5(6) of Decision 1999/468/EC shall be set at three months.

Ö 3. Where reference is made to this paragraph, Articles 5 and 7 of Decision 1999/468/EC shall apply. Õ

Ö The period laid down in Article 5(6) of Decision 1999/468/EC shall be set at 15 days. Õ

ê

Article 22

Directive 90/426/EEC, as amended by the acts listed in Annex V, Part A, is repealed, without prejudice to the obligations of the Member States relating to the time-limits for transposition into national law of the Directives set out in Annex V, Part B.

References to the repealed Directive shall be construed as references to this Directive and shall be read in accordance with the correlation table in Annex VI.

Article 23

This Directive shall enter into force on the twentieth day following that of its publication in the Official Journal of the European Union .

It shall apply from 2 January 2010.

ê 90/426/EEC

Article 24

This Directive is addressed to the Member States.

Done at Brussels, […]

For the Council

The President

[…]

ê 90/426/EEC

ANNEX I

COMPULSORILY NOTIFIABLE DISEASES

The following diseases are compulsorily notifiable:

- Dourine

- Glanders

- Equine encephalomyelitis (of all types, including VEE)

- Infectious anaemia

- Rabies

- Anthrax

- African horse sickness

- Vesicular stomatitis

__________

ê 92/130/EEC Art 1 and Annex (adapted)

è1 2001/298/EC Art. 1(1) and Annex I pt. 2(a)

ANNEX II

Ö MODEL Õ

HEALTH Ö ATTESTATION Õ (a)

Passport No ……………..

I, the undersigned, certify (b) that the animal Ö identified Õ above meets the following requirements:[cette attestation s'applique à tout équidé, donc "equine animal" est trop limité; si vous avez l'équivalent d' "équidé", laissez votre version inchangée; sinon, utilisez "animal". Identified a éte utilisé pour faire le lien avec le passport number; si vous avez l´équivalent de "indiqué/ désigné /mentionné", pas besoin d´adapter]

(a) it has been examined today and shows no clinical sign of disease;

(b) it is not intended for slaughter under a national programme or contagious or infectious disease eradication;

(c) – it does not come from the territory or part of the territory of a Member State which is the subject of restrictions for reasons of African horse sickness , or

it comes from the territory or part of the territory of a Member State which was subject to prohibition for animal health reasons and has undergone, with satisfactory results, the tests provided for in Article 5(5) of Directive [ …/…/EC ] in the quarantine station of ………… between …………. and ……………… (c);

- it is not vaccinated against African horse sickness, or

it was vaccinated against African horse sickness on ………….. (c) (d);

(d) it has not come from a holding which was subject to prohibition for animal health reasons nor had contact with equidae from a holding which was subject to prohibition for animal health reasons:

- during six months in the case of equidae suspected of having contracted dourine, beginning on the date of the last actual or possible contact with a sick animal. However, in the case of a stallion, the prohibition shall apply until the animal is castrated,

- during six months in the case of glanders or equine encephalomyelitis, beginning on the day on which the equidae suffering from the disease in question are slaughtered,

- in the case of infectious anaemia, until the date on which, the infected animals having been slaughtered, the remaining animals have shown a negative reaction to two Coggins tests carried out three months apart,

- during six months from the last case, in the case of vesicular stomatitis,

- during one month from the last case, in the case of rabies,

- during 15 days from the last case, in the case of anthrax,

- if all the animals of species susceptible to the diseases located on the holding have been slaughtered and the premises disinfected during 30 days, beginning on the day on which the animals were destroyed and the premises disinfected, except in the case of anthrax, where the period of prohibition is 15 days;

(e) to the best of my knowledge, it has not been in contact with equidae suffering from an infectious or contagious disease in the 15 days prior to this declaration;

è1 (f) at the time of the inspection it was fit to be transported on the intended journey in accordance with the provisions of Regulation (EC) No 1/2005 (e). ç

Date | Place | Stamp and signature of the official veterinarian (1) |

(1) Name in block capitals and capacity. |

________

(a) This attestation is not required where there is a bilateral agreement in accordance with Article 6 of Directive […/…/EC].

(b) Valid for 10 days.

(c) Delete whichever does not apply.

(d) The vaccination date must be entered in the passport.

è1 (e) This statement does not exempt transporters from their obligations in accordance with Community provisions in force in particular regarding the fitness of animals to be transported. ç

_____________

ê 92/130/EEC Art 1 and Annex (adapted)

è1 2001/298/EC Art. 1(1) and Annex I, pt. 2(b)

è2 1994 Act of Accession Art. 29 and Annex I, p. 132

è3 2003 Act of Accession Art. 20 and Annex II, p. 381

è4 2006/104/EC Art. 1 and Annex, pt. I.2.

ANNEX III

MODEL

HEALTH CERTIFICATE

For trade between Member States

EQUIDAE

No: ……………………………

Member State of dispatch: ………………………………………………………………………………………….

Ministry responsible: ………………………………………………………………………………………………..

Territorial Department responsible: ………………………………………………………………………………...

I. Number of equidae: ……………………………………………………………………………...

II. Identification of equidae:

Number of equidae (1) | Species horse, ass, mule, hinny | Breed Age Sex | Method of identification and identification (2) |

(1) In the case of animals for slaughter, nature of the special mark. (2) A passport identifying the equine animal may Ö accompany the animal for which this certificate is issued Õ provided that its number is stated. |

III. Origin and destination of animal/s:

The animal/s is/are to be sent from: ……………………………………………………………………………………………………… (Place of export) to: …………………………………………………………………………………………………… (Member State and place of destination) Name and address of consignor: …………………………………………………………………… …………………………….....…………………………………………………………………….. Name and address of consignee: …………………………………………………………………… ……………………………………………………………………………………………………… |

IV. Health information (a)

I, the undersigned, certify that the animal/s described above meet/s the following requirements:

1. it/they have been examined today and show/s no clinical sign of disease;

2. it/they is/are not intended for slaughter under a national programme of contagious or infectious disease eradication;

3. – it/they does/do not come from the territory or part of the territory of a Member State which is the subject of restrictions for reasons of African horse sickness,

or

it/they come/s from the territory or part of the territory of a Member State which was the subject of restrictions for reasons of African horse sickness and has/have undergone, with satisfactory results, the tests provided for in Article 5(5) of Directive […/…/EC] in the quarantine station of …………………. between ………… and …………………… (b),

- it/they is/are not vaccinated against African horse sickness,

or

it/they was/were vaccinated against African horse sickness on …………………… (b);

4. it/they has/have not come from a holding which was subject to prohibition for animal health reasons nor had contact with equidae from a holding which was subject to prohibition for animal health reasons:

- during six months in the case of equidae suspected of having contracted dourine, beginning on the date of the last actual or possible contact with a sick animal. However, in the case of a stallion, the prohibition shall apply until the animal is castrated,

- during six months in the case of glanders or equine encephalomyelitis, beginning on the day on which the equidae suffering from the disease in question are slaughtered,

- in the case of infectious anaemia, until the date on which, the infected animals having been slaughtered, the remaining animals have shown a negative reaction to two Coggins tests carried out three months apart,

- during six months from the last case, in the case of vesicular stomatitis,

- during one month from the last case, in the case of rabies,

- during 15 days from the last case, in the case of anthrax,

- if all the animals of species susceptible to the disease located on the holding have been slaughtered and the premises disinfected during 30 days, beginning on the day on which the animals were destroyed and the premises disinfected, except in the case of anthrax, where the period of prohibition is 15 days;

5. to the best of my knowledge, it/they has/have not been in contact with equidae suffering from an infectious or contagious disease in the 15 days prior to this declaration;

è1 6. at the time of the inspection it/they was/were fit to be transported on the intended journey in accordance with the provisions of Regulation (EC) No 1/2005 ( c). ç

[pic]

(Name in capital letters and capacity of signing veterinarian) ( d) |

____________ (a) This information is not required where there is a bilateral agreement in accordance with Article 6 of Directive […/…/EC]. (b) Delete whichever does not apply. (c) This statement does not exempt transporters from their obligations in accordance with Community provisions in force in particular regarding the fitness of animals to be transported. (d) In Belgium: 'Inspecteur vétérinaire' or 'Inspecteur Dierenarts'; è4 in Bulgaria: 'ветеринарен инспектор'; ç è3 in the Czech Republic: 'veterinární inspektor'; ç in Denmark: 'Embedsdyrlaege'; in Germany: 'Beamteter Tierarzt'; è3 in Estonia: 'veterinaarjärelevalve ametnik'; ç in Ireland: 'Veterinary Inspector'; in Greece: 'Επίσημος κτηνίατρος'; in Spain: 'Inspector Veterinário'; in France: 'Vétérinaire officiel'; in Italy: 'Veterinario ufficiale'; è3 in Cyprus: 'Επίσημος κτηνίατρος'; in Latvia: 'veterinarais inspektors'; in Lithuania: 'veterinarijos inspektorius'; ç in Luxembourg: 'Inspecteur vétérinaire'; è3 in Hungary: 'hatósági állatorvos'; in Malta: 'veterinarju ufficjali'; ç in the Netherlands: 'Officieel Dierenarts'; è2 in Austria: 'Amtstierarzt'; ç è3 in Poland: 'urzedowy lekarz weterynarii'; ç in Portugal: 'Inspector Veterinário'; è4 in Romania: 'medic veterinar autorizat'; ç è3 in Slovenia: 'veterinarski inspektor'; in Slovakia: 'veterinárny inspektor'; ç è2 in Finland: 'kunnaneläinlääkäri', 'kaupungineläinlääkäri' or ''läänineläinlääkäri' / 'kommunalveterinär', 'stadsveterinär' or 'länsveterinär', in Sweden: 'länsveterinär', 'distriktsveterinär' or 'gränsveterinär'; ç in the United Kingdom: 'Veterinary Inspector'. |

__________

ê 2002/160/EC Art. 1 and Annex

ANNEX IV

AFRICAN HORSE SICKNESS

DIAGNOSIS

Reagents for the enzyme-linked immunosorbent assays (ELISA) described below may be obtained from the European Community Reference Laboratory or the OIE Reference Laboratories for African horse sickness.

1. COMPETITIVE ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST)

Competitive ELISA is used to detect specific AHSV antibodies in sera from any species of equidae. The broad spectrum, polyclonal, immune anti-AHSV guinea-pig serum (hereinafter “guinea-pig antiserum”) is serogroup specific and is able to detect all known serotypes of AHS virus.

The principle of the test is the interruption of the reaction between AHSV antigen and a guinea-pig antiserum by a test serum sample. AHSV antibodies in the test serum sample will compete with those in the guinea-pig antiserum resulting in a reduction in the expected colour (following the addition of enzyme labelled anti-guinea-pig antibody and substrate). Sera can be tested at a single dilution of 1 in 5 (spot test method) or may be titrated (serum titration method) to give dilution end-points. Inhibition values higher than 50 % may be regarded as positive.

The test protocol described hereinafter is used in the Regional Reference Laboratory for African horse sickness in Pirbright, United Kingdom.

1.1. Test procedure

1.1.1. Preparation of plates

1.1.1.1. Coat ELISA plates with AHSV antigen extracted from infected cell cultures and diluted in carbonate/bicarbonate buffer, pH 9,6. Incubate the ELISA plates overnight at 4 °C.

1.1.1.2. Wash plates three times by flooding and emptying the wells with phosphate buffered saline (PBS), pH 7, 2 to 7,4 pH, and blot dry on adsorbent paper.

1.1.2. Control wells

1.1.2.1. Titrate the positive control sera in a twofold dilution series, from 1 in 5 to 1 in 640, across column 1 in blocking buffer (PBS containing 0,05 % (v/v) Tween-20, 5,0 % (w/v) skimmed-milk powder (Cadbury's MarvelTM) and 1 % (v/v) adult bovine serum) to give a final volume of 50 μl/well.

1.1.2.2. Add 50 μl of the negative control serum at a dilution of 1 in 5 (10 μl serum + 40 μl blocking buffer) to wells A and B of column 2.

1.1.2.3. Add 100 μl/well of blocking buffer to wells C and D of column 2 (blank).

1.1.2.4. Add 50 μl of blocking buffer to wells E, F, G and H of column 2 (guinea pig control).

1.1.3. Spot test method

1.1.3.1. Add a 1 in 5 dilution of each test serum in blocking buffer to duplicate wells of columns 3 to 12 (10 μl sera + 40 μl blocking buffer).

or

1.1.4. Serum titration method

1.1.4.1. Prepare a twofold dilution series of each test sample (1 in 5 to 1 in 640) in blocking buffer across eight wells of single columns (3 to 12).

then

1.1.5 . Add 50 μl of guinea pig antisera, pre-diluted in blocking buffer, to all wells except the blank wells of the ELISA plate (all wells now contain a final volume of 100 μl).

1.1.5.1. Incubate for 1 hour at 37 °C on an orbital shaker.

1.1.5.2. Wash plates three times and blot dry as before.

1.1.5.3. Add 50 μl of rabbit anti-guinea-pig horseradish peroxidase (HRP) conjugate pre-diluted in blocking buffer to each well.

1.1.5.4. Incubate for 1 hour at 37 °C on an orbital shaker.

1.1.5.5. Wash plates three times and blot dry as before.

1.1.6. Chromogen

Prepare the chromogen OPD (OPD = ortho-phenyldiamine) solution according to the manufacturers' instructions (0,4 mg/ml in sterile distilled water) just before use. Add substrate (hydrogen peroxide = H2O2) to give a final concentration of 0,05 % (v/v) (1 in 2000 of a 30 % solution of H2O2). Add 50 μl of the OPD solution to each well and leave plates on the bench for 10 minutes at ambient temperature. Stop the reaction by the addition of 50 μl/well of 1M sulphuric acid (H2SO4).

1.1.7. Reading

Read spectrophotometrically at 492 nm.

1.2. Expression of results

1.2.1. Using a software package print out the optical density (OD) values, and the percentage inhibition (PI) for test and control sera based on the mean value recorded in the four guinea pig control wells. The data expressed as OD and PI values are used to determine whether the test has performed within acceptable limits. The upper control limits (UCL) and lower control limits (LCL) for the guinea pig control are between OD values 1,4 and 0,4 respectively. The end-point titre for the positive control based on 50 % PI should be 1 in 240 (within a range from 1 in 120 to 1 in 480). Any plate that fails to conform to the above criteria must be rejected. However, if the positive control serum titre is greater than 1 in 480 and the test samples are still negative then the negative test samples can be accepted.

The duplicate negative control serum wells and the duplicate blank wells should record PI values between + 25 % and – 25 %, and between + 95 % and + 105 %, respectively. Failure to be within these limits does not invalidate the plate but does suggest that background colour is developing.

1.2.2. The diagnostic threshold (cut-off value) for test sera is 50 % (PI 50 %). Samples recording PI values greater than 50 % are recorded as positive. Samples recording PI values lower than 50 % are recorded as negative.

Samples that record PI values above and below the threshold for the duplicate wells are considered doubtful. Such samples may be re-tested in the spot test and by titration. Positive samples may also be titrated to provide an indication of the degree of positivity.

Spot test layout

Test sera

2. INDIRECT ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST)

The test described hereinafter is in accordance with the test description in Chapter 2.1.11 of the OIE Manual of Standards for Diagnostic Tests and Vaccines , fourth edition, 2000.

The recombinant VP7 protein has been used as antigen for AHS virus antibody determination with a high index of sensitivity and specificity. Other advantages are that it is stable and not infective.

2.1. Test procedure

2.1.1. Solid phase

2.1.1.1. ELISA plates are coated with recombinant AHSV-4 VP7 diluted in carbonate/bicarbonate buffer, pH 9,6. Incubate plates overnight at 4 °C.

2.1.1.2. Wash the plates five times with distilled water containing 0,01 % (v/v) Tween 20 (washing solution). Gently tap the plates onto absorbent material to remove any residual wash.

2.1.1.3. Block the plates with phosphate buffered saline (PBS) + 5 % (w/v) skimmed milk (Nestlé Dry Skim MilkTM), 200 μl/well, for 1 hour at 37 °C.

2.1.1.4. Remove the blocking solution and gently tap the plates onto absorbent material.

2.1.2. Test samples

2.1.2.1. Serum samples to be tested, and positive and negative control sera, are diluted 1 in 25 in PBS + 5 % (w/v) skimmed milk + 0,05 % (v/v) Tween 20, 100 μl per well. Incubate for 1 hour at 37 °C.

For titration, make a twofold dilution series from 1 in 25 (100 μl/well), one serum per plate column, and do the same with positive and negative controls. Incubate for 1 hour at 37 °C.

2.1.2.2. Wash the plates as described in step 2.1.1.2.

2.1.3. Conjugate

2.1.3.1. Dispense 100 μl/well of horseradish-peroxidase (HRP) -conjugated anti-horse gamma-globulin diluted in PBS + 5 % milk + 0,05 % Tween 20, pH 7,2. Incubate for 1 hour at 37 °C.

2.1.3.2. Wash the plates as described in step 2.1.1.2.

2.1.4. Cromogen/Substrate

2.1.4.1. Add 200 μl/well of chromogen/substrate solution (10 ml of 80,6 mM DMAB (dimethyl aminobenzaldehyde) + 10 ml of 1,56 mM MBTH (3-methyl-2-benzo-thiazoline hydrazone hydrochlorid) + 5 μl H2O2)

Colour development is stopped by adding 50 μl of 3N H2SO4 after approximately 5 to 10 minutes (before the negative control begins to be coloured).

Other chromogens such as ABTS (2,2'-Azino-bis-[3-ethylbenzothiazoline-6-sulphonic acid]), TMB (tetramethyl benzidine), or OPD (ortho-phenyldiamine) can also be used.

2.1.4.2. Read the plates at 600 nm (or 620 nm).

2.2. Interpretation of the results

2.2.1. Calculate the cut-off value by adding 0,6 to the value of the negative control (0,6 is the standard deviation derived with a group of 30 negative sera).

2.2.2. Test samples giving absorbance values lower than the cut-off are regarded as negative.

2.2.3. Test samples giving absorbance values greater than the cut-off + 0,15 are regarded as positive.

2.2.4. Test samples giving intermediate absorbance values are doubtful and a second technique must be employed to confirm the result.

3. BLOCKING ELISA FOR THE DETECTION OF ANTIBODIES TO AFRICAN HORSE SICKNESS VIRUS (AHSV) (PRESCRIBED TEST)

The blocking ELISA is designed to detect specific AHSV antibodies in sera from any susceptible species. VP7 is the major, antigenic, viral protein of AHSV, and is conserved within the nine serotypes. Because the monoclonal antibody (Mab) is also directed against the VP7, the assay will give a high level of sensitivity and specificity. Further, the recombinant VP7 antigen is completely innocuous and therefore guarantees a high degree of safety.

The principal of the test is the interruption of the reaction between the recombinant VP7, as the antigen bound to the ELISA plate and the conjugated Mab specific for the VP7. Antibody in the test sera will block the reaction between the antigen and the Mab resulting in a reduction in colour.

The test described hereinafter is carried out in the European Community Reference Laboratory for African horse sickness in Algete, Spain.

3.1. Test procedure

3.1.1. ELISA plates

3.1.1.1. Coat ELISA plates with recombinant AHSV-4 VP7 diluted in carbonate/bicarbonate buffer, pH 9,6. Incubate overnight at 4 °C.

3.1.1.2. Wash the plates five times with phosphate buffered saline (PBS) containing 0,05 % (v/v) Tween 20 (PBST).

3.1.1.3. Stabilise the plate by treatment with a stabilising solution (in order to allow long term storage at 4 °C without loss of activity) and blot dry onto absorbent material.

3.1.2. Test samples and controls

3.1.2.1. For screening: | dilute test sera and controls 1 in 10 directly on the plate in PBST to give a final volume 100 μl/well. Incubate for 1 hour at 37 °C. |

3.1.2.2. For titration: | prepare a twofold dilution series of test sera and positive controls (100 μl/well) from 1 in 10 to 1 in 1280 across eight wells. Negative control is tested at 1 in 10 dilution. |

3.1.3. Conjugate

Add 50 μl/well of pre-diluted horseradish-peroxidase (HRP) -conjugated Mab (monoclonal antibodies specific for VP7) to each well and mix gently to ensure homogeneity. Incubate for 30 minutes at 37 °C.

3.1.4 . Wash the plates five times with PBST and blot dry as above.

3.1.5. Chromogen/Substrate

Add 100 μl/well of chromogen/substrate solution (1 ml of ABTS (2,2'-Azino-bis-[3-ethylbenzothiazoline-6-sulphonic acid]) 5 mg/ml + 9 ml of substrate buffer (0,1 M Phosphate-Citrate buffer of pH 4 containing 0,03 % H2O2] and incubate for 10 minutes at room temperature. Colour development is stopped by adding 100 μl/well of 2 % (w/v) SDS (sodium dodecyl sulphate).

3.1.6. Reading

Read at 405 nm in an ELISA reader.

3.2. Interpretation of the results

3.2.1. Assay validation

The test is valid when the optical density (OD) of negative control (NC) is higher than 1,0 and the OD of positive control (PC) is lower than 0,2.

3.2.2. Cut-off calculation

Positive cut off | = | NC - ((NC - PC) x 0,3) |

Negative cut off | = | NC - ((NC - PC) x 0,2) |

Where, NC is the OD of the negative control and PC the OD of positive control.

3.2.3. Interpretation of results

Samples with OD lower than positive cut-off should be considered as positives to AHSV antibodies.

Samples with OD higher than negative cut-off should be considered negatives for AHSV antibodies.

Samples with OD between these two values should be considered doubtful and the animals re-sampled after two to three weeks.

__________

é

ANNEX V

Part A

Repealed Directive with list of its successive amendments (referred to in Article 22)

Council Directive 90/426/EEC (OJ L 224, 18.8.1990, p. 42) |

Council Directive 90/425/EEC (OJ L 224, 18.8.1990, p. 29) | only Article 15(3) |

Council Directive 91/496/EEC (OJ L 268, 24.9.1991, p. 56) | only as regards the reference to Directive 90/426/EEC in Article 26(2) |

Commission Decision 92/130/EEC (OJ L 47, 22.2.1992, p. 26) |

Council Directive 92/36/EEC (OJ L 157, 10.6.1992, p. 28) | only Article 1 |

Point V.E.I.A.3 of Annex I to the 1994 Act of Accession (OJ C 241, 29.8.1994, p. 132) |

Commission Decision 2001/298/EC (OJ L 102, 12.4.2001, p. 63) | only as regards the reference to Directive 90/426/EEC in Article 1(1), and Annex I, pt. 2 |

Commission Decision 2002/160/EC (OJ L 53, 23.2.2002, p. 37) |

Council Regulation (EC) No 806/2003 (OJ L 122, 16.5.2003, p. 1) | only Annex III, pt. 10 |

Point 6.B.I.16 of Annex II to the 2003 Act of Accession (OJ L 236, 23.9.2003, p. 381) |

Council Directive 2004/68/EC (OJ L 139, 30.4.2004, p. 321) | only Article 15 |

Council Directive 2006/104/EC (OJ L 363, 20.12.2006, p. 352) | only Annex, pt. I.2. |

Council Directive 2008/73/EC (OJ L 219, 14.8.2008, p. 40) | only Article 7 |

Part B

List of time-limits for transposition into national law (referred to in Article 22)

Directive | Time-limit for transposition |

90/426/EEC | 1 January 1992 |

90/425/EEC | 1 July 1992 |

91/496/EEC | 1 July 1992 |

92/36/EEC | 31 December 1992 |

2004/68/EC | 19 November 2005 |

2006/104/EC | - |

2008/73/EC | 1 January 2010 |

_____________

ANNEX VI

Correlation Table

Directive 90/426/EEC | This Directive |

Article 1 | Article 1 |

Article 2(a) and (b) | Article 2(a) and (b) |

Article 2(c) | Article 2(c)(i) and (ii) |

Article 2(d) to (i) | Article 2(d) to (i) |

Article 3 | Article 3 |

Article 4(1), (2) and (3) | Article 4(1), (2) and (3) |

Article 4(4)(i) and (ii) | Article 4(4)(a) and (b) |

Article 4(5)(a), first to sixth indents | Article 4(5)(a)(i) to (vi) |

Article 4(5)(b) | Article 4(5)(b) |

Article 4(6), first subparagraph, first to eighth indents | Article 4(6), first subparagraph, (a) to (h) |

Article 4(6), second and third subparagraphs | Article 4(6), second and third subparagraphs |

Article 5(1) | Article 5(1) |

Article 5(2)(a) | Article 5(2), first subparagraph (a) and (b) |

Article 5(2)(b) | Article 5(2), second subparagraph (a) and (b) |

Article 5(2)(c) | Article 5(3) |

Article 5(2)(d) | Article 5(4) |

Article 5(3)(a) and (b) | Article 5(5)(a) and (b) |

Article 5(3)(c), first and second indents | Article 5(5)(c), first subparagraph, (i) and (ii) |

Article 5(3)(c), second indent, last sentence | Article 5(5)(c), second subparagraph |

Article 5(3)(d) and (e) | Article 5(5)(d) and (e) |

Article 6 | Article 6 |

Article 7 | Article 7 |

Article 8(1), first subparagraph, first and second indents | Article 8(1)(a) and (b) |

Article 8(1), second subparagraph | Article 8(2) |

Article 8(2) | Article 8(3) |

Article 9 | Article 9 |

Article 10 | Article 10 |

Article 11(1) | Article 11 |

Article 11(2) | - |

Article 12 | Article 12 |

Article 13 | Article 13 |

Article 14 | Article 14 |

Article 15 | Article 15 |

Article 16(1)(a) to (f) | Article 16(1)(a) to (f) |

Article 16(1), final sentence | - |

Article 16(2) | Article 16(2) |

Article 17 | Article 18 |

Article 18 | Article 17 |

Article 19(i) to (iv) | Article 19(a) to (d) |

Article 22 | - |

Article 23 | Article 20 |

Article 24(1) and (2) | Article 21(1) and (2) |

Article 24(3) | - |

Article 25(1) and (2) | Article 21(1) and (3) |

Article 26 | - |

Article 27 | - |

- | Article 22 |

- | Article 23 |

Article 28 | Article 24 |

Annex A | Annex I |

Annex B | Annex II |

Annex C | Annex III |

Annex D | Annex IV |

- | Annex V |

- | Annex VI |

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[1] COM(87) 868 PV.

[2] See Annex 3 to Part A of the Conclusions.

[3] Carried out pursuant to the Communication from the Commission to the European Parliament and the Council – Codification of the Acquis communautaire, COM(2001) 645 final.

[4] Annex V, Part A of this proposal.

[5] OJ C […], […], p. […].

[6] OJ L 224, 18.8.1990, p. 42.

[7] See Annex V, Part A.

[8] OJ L 3, 5.1.2005, p. 1.

[9] OJ L 224, 18.8.1990, p. 29.

[10] OJ L 184, 17.7.1999, p. 23.

[11] OJ L 224, 18.8.1990, p. 55.

[12] OJ L 157, 10.6.1992, p. 19.

[13] OJ 121, 29.7.1964, p. 1977/64.

[14] OJ L 31, 1.2.2002, p. 1.