[en] Labelling blood granulocytes presents two fields of interest: granulocyte kinetics and localization of deep-seated sites of infection. The authors have developed a new method of granulocyte separation and labelling. Its validity was tested in-vitro by a scanning electron microscope and by bactericidal and chemotactic assays. (orig.)

[en] An optimized procedure is described for isolation and high-efficiency radiolabeling of leukocytes using ¹¹¹In-oxine. The chief advantages over conventional methods include virtually no loss of leukocytes during washing and separation steps; a significant reduction in the time required to prepare leukocytes for radiolabeling compared to non-hemolytic preparations; a 28% increase in the average labeling efficiency obtained using ¹¹¹In-oxine; > 95% cell viability as measured by the trypan blue exclusion test; elimination of contaminating red blood cells from the leukocyte pellet prior to labeling; and 80% survivability at 15 min post injection (measured as per cent of blood activity on leukocyte fraction). (author)

[en] This patent describes an improved method for radio-labelling leucocytes with Indium In-111 oxine. It comprises separating the leucocytes from whole blood for obtaining separated leucocytes mixed with residual red blood cells; and then labelling the separated leucocytes with Indium In-111 oxine; wherein the improvement comprises the following further step: depleting residual red blood cells from the separated leucocytes by resuspending the leucocytes in an isotonic saline solution, then rocking the resuspended leucocytes for causing the leucocytes to preferentially settle out, and then removing residual red blood cells which remain suspended within the supernatant isotonic saline solution

[en] The range of leucocyte labelling efficiencies with ¹¹¹In-oxine for a group of patients extended significantly below that obtained for a series of labellings of the same normal blood from a volunteer. A retrospective analysis was made of the results in the two groups to identify the cause of this difference in range. The labelling efficiency for patients did not vary with the volume of ¹¹¹In-oxine, and was independent of the whole blood leukocyte concentration. The difference between the average labelling efficiencies obtained for a group of patients and the normal series labelled by the same operator was more significant than the difference in average labelling efficiencies obtained by different operators. It was concluded that biological variation in patients' blood, rather than operator technique, must have been a more important cause of the difference in labelling efficiency range between patients and normal. It was also concluded that variations of contaminant platelet-bound activity and of plasma viscosity were greater in the patient group than the normal series, and contributed to this difference in labelling efficiency range. (author)

[en] Copper 8-hydroxyquinoline-2-carboxaldehyde–thiosemicarbazide (CuHQTS) and copper 8-hydroxyquinoline-2-carboxaldehyde–4,4-dimethyl-3-thiosemicarbazide (CuHQDMTS) are two copper thiosemicarbazone complexes with potent anticancer activity on cisplatin-resistant neuroblastoma cells. This study aimed to evaluate anti-prostate cancer activity of these two copper complexes in vitro. Both CuHQTS and CuHQDMTS inhibited proliferation of prostate cancer cells and showed cytotoxicity on prostate cancer cells carrying green fluorescent protein (GFP) by fluorescent microscopic imaging. The findings of this study demonstrated anti-prostate cancer activity of CuHQTS and CuHQDMTS and suggested that GFP-carrying prostate cancer cells might be used for testing anticancer activity of copper complexes by fluorescent microscopic imaging.

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[en] The determination of trace amounts iron is imperative in various fields including nuclear fuel characterization. Despite various reports on developed methodologies, there exists an ever increasing interest to newer methods which are rapid and simple. In this paper, flow injection analysis of iron using flow injection analyzer with UV Visible detector has been discussed. Since the method is dependent on formation of a coloured species between iron and a chromophoric reagent, it was necessary to optimize the chromophore in addition to other parameters. Different reagents like orthophenanthroline, 8 Hydroxy Quinoline and xylenol orange (XO) were evaluated and further studies were carried out using XO. The optimized of XO and acidity were 0.005% and 0.05 M respectively. A calibration was obtained for a concentration range of 10-80 ng of iron with a R2 of 0.9934 (Fig.2). It is seen that upto 1 mg/mL of uranium, the calibration plots are quite similar. But the sensitivity was severely affected at higher uranium concentrations (5 and 10 mg/mL). The limit of detection was 1 ng and the precision was found to be 1.8%. (author)

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