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AbstractAbstract
[en] The harlequin-staining method has been used to study the cell kinetic of goat peripheral blood lymphocytes stimulated by phytohemagglutinin and to assess their radiosensitivity. At 48 h, the standardized culture time employed for human lymphocytes, 71% of the goat lymphocytes are in first mitosis, 23% are in second mitosis and 5% in third. Irradiation with 200 rads X-rays induces an average of 24,5 dicentric chromosomes per hundred cells in first mitosis
[fr]
La methode de marquage par la 5-bromodesoxyuridine (BUDR) a ete utilisee pour etudier la cinetique des lymphocytes de la chevre, actives par la phytohemagglutinine, et pour estimer leur radiosensibilite. A 48 heures, le temps de culture normalement utilise pour les lymphocytes humains, 71% des lymphocytes de la chevre sont en premiere mitose. 23% sont en deuxieme division et 5% en troisieme division. L'exposition a 200 rad de rayons X produit une moyenne de 24,5 chromosomes dicentriques par cent cellules en premiere divisionOriginal Title
Cinetique cellulaire et radiosensibilite des lymphocytes de chevre stimules par la phytohemagglutinine
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Journal Article
Journal
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales; ISSN 0037-9026; ; v. 176(1); p. 82-86
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AGGLUTININS, ANIMAL CELLS, ANIMALS, BIOLOGICAL MATERIALS, BLOOD, BLOOD CELLS, BODY FLUIDS, CARBOHYDRATES, CELL DIVISION, CHROMOSOMES, CONNECTIVE TISSUE CELLS, DOMESTIC ANIMALS, ELECTROMAGNETIC RADIATION, HEMAGGLUTININS, IONIZING RADIATIONS, LEUKOCYTES, MAMMALS, MITOGENS, MUCOPROTEINS, ORGANIC COMPOUNDS, POLYSACCHARIDES, PROTEINS, RADIATIONS, RUMINANTS, SACCHARIDES, SOMATIC CELLS, VERTEBRATES
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