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[en] The labelling reaction of human growth hormone (hGH) with 125I and its chromatographic purification have been studied with emphasis on the reproducibility of the yields, quantitaTive recoveries and resulting activities. Through the accurate standardization of a monitoring technique, it is confirmed that there are no significant losses in radioactivity or protein during the labelling or purification process. By strict control of the reaction conditions a fairily good reproducibility is also obtained in the labelling of various hGH extracts with diferent 125I shipments used after short or long storage. Finally, the specific activity (or absolute mass) of the radioiodinated protein is determined by this Analysis of the Reaction Mixture and compared to the widely used radioimmunological assay (Self-displacement). (M.A.C.)
[pt]
Estuda-se a reacao de marcacao do hormonio de crescimento humano (hCH) com 125I e sua purificacao cromatografica, focalizando-se especialmete a reprodutibilidade dos rendimentos, as recuperacoes quantitativas e as atividades especicas obtidas. Confirma-se, atraves da rigorosa padronizacao de uma tecnica de monitoracao, que nao ha perdas significativas de radioatividade ou proteina durante o processo de marcacao ou purificacao. Obtem-se uma reprodutibilidade satisfatoria na marcacao de varios extratos de hCH usando-se diferentes embarques de 125I apos curtos ou longos periodos de estocagem deste radioisotopo. Determina-se a atividade especifica (ou massa absoluta) da proteina radioiodada mediante a analise da mistura de reacao, comparando-se a obtida pelo metodo radioimunologico (Self-displacement) mais usado. (M.A.C.)Journal
Revista do Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo; ISSN 0041-8781; 
; CODEN RHCFA; v. 43(2); p. 93-100
; CODEN RHCFA; v. 43(2); p. 93-100