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AbstractAbstract
[en] Gasoy 17, Chiang Mai 60 and BSR 101 were chosen among 14 soybean cultivars for mutation induction experiment and in vitro selection for soybean lines tolerant to aluminum and trace element deficiencies. Young pods from all 3 cultivars were irradiated with gamma-rays at different doses and immature cotyledons were cultured in an induction medium. Somatic embryogenesis was markedly enhanced by irradiation with 1-5 Gy in Chiang Mai 60 and BSR 101 and with 1-3 Gy in Gasoy 17. The somatic embryos were multiplied by culturing in liquid medium (pH 5.8) for 5 weeks. The somatic embryos of Gasoy 17 and Chiang Mai 60 were cultured for tolerance to aluminum in media containing different al levels (5.6 11.2 and 16.8 ppm). Al toxicity resulted in a low percentage of fresh weight of the somatic embryos. Somatic embryos selected at different Al's were cultured in a regeneration medium but no plantlet has emerged so far. Selection for tolerance to trace element deficiencies was done by culturing somatic embryos of BSR 101 in high pH medium (pH 6-8). Under high pH the availability of iron in the medium was noticeably decreased through precipitation of Fe-compounds. Somatic embryos selected at different pH were cultured in the regeneration medium and 8 regenerated plants were grown to maturity for further investigation on tolerance to trace element deficiencies in their progenies
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Office of Atomic Energy for Peace, Bangkok (Thailand); 761 p; Dec 1996; [13 p.]; 6. Conference on Nuclear Science and Technology; Bangkok (Thailand); 2-4 Dec 1996; ALSO AVAILABLE FROM OFFICE OF ATOMIC ENERGY FOR PEACE, BANGKOK (THAILAND)
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Miscellaneous
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Conference; Numerical Data
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