[en] The combination of 2 techniques, i, e., pollination after the destruction of chromosomes in pollen grains with ionizing radiations and in vitro culture of crossed ovules, was applied to interspecific hybridization between N.repanda and N. tabacum in order to investigate the possibility of overcoming hybrid inviability in the cross-combinations. By using these techniques, it became possible to obtain aneuploid hybrids, which had never been reported previously