[en] Objective: To prepare ¹⁸F-2-fluoropropionyl(FP)-Lladtthhrpwt(¹⁸F-FP-ZS-6) by a multifunctional ¹⁸F radiolabeling module and investigate its in vivo distribution in human lung cancer cell (NCI-H1299)-bearing nude mice by microPET. Methods: Using a phage display peptide library to screen and identify Lladtthhrpwt (ZS-6) which has an affinity for human lung cancer. PET-MF-2V-IT-I synthesizer was used for the synthesis and purification of ¹⁸F-FP-ZS-6. MicroPET studies were carried out in NCI-H1299-bearing nude mice after injection of ¹⁸F-FP-ZS-6. Results: 4-nitrophenyl 2-[¹⁸F] fluoropropionate (¹⁸F-NFP) was prepared by 'one-pot' radiochemical procedure. The radiochemical yield of ¹⁸F-FP-ZS-6 was (5 ± 2) % (n = 3, decay-corrected) from ¹⁸F and the radiochemical purity was more than 95%. MicroPET studies showed that the uptake of ¹⁸F-FP-ZS-6 was high in the tumor and stomach of NCI-H1299-bearing nude mice model. The tumor uptake of ¹⁸F-FP-ZS-6 was 0.329, 0.350, 0.405, 0.433, 0.420, 0.415, 0.402 , 0.403, 0.390 %ID/g at 5, 15, 25, 35, 45, 55, 65, 75, 85 min post-injection, respectively. The tumor uptake peak was at 35 min post-injection and then ¹⁸F-FP-ZS-6 was cleared slowly. Conclusions: ¹⁸F-FP-ZS-6 could be successfully prepared. It might be a potential tracer for imaging human lung cancer. (authors)