[en] Objective: To study the factors affecting the synthesis of ¹⁸F-MyoZone, and to evaluate its potential as a myocardial perfusion imaging (MPI) agent in normal Chinese mini-swine. Methods: ¹⁸F-MyoZone was prepared by substituting the leaving group toluenesulfonyloxy (OTs) from the precursor compound with ¹⁸F-fluoride (¹⁸F-F^-). The conditions affecting the labeling yield were studied by varying the amount of K₂C0₃ and precursor compound, ¹⁸F-fluorination reaction time and temperature. PET was performed at 5, 30, 60 and 120 min post-injection on normal Chinese mini-swine. Results: The doses of K₂C0₃ and precursor, the reaction time and the reaction temperature could affect the labeling yield of ¹⁸F-MyoZone, especially K₂C0₃. The optimized synthetic condition was 1.0 mg K₂C0₃, 2.0 mg mpp2-OTs, 20 min reaction time at 90 ℃. The total radio-synthesis time in this condition was 60 min. The uncorrected radiochemical yield was (24.0 ± 5.1)%. The radiochemical purity was > 98%, PET imaging showed that ¹⁸F-MyoZone had high initial uptake (SUV = 8.17 ± 1.83 at 5 min post-injection) and good retention (SUV = 5.78 ± 0.99 at 120 min post-injection) in the heart. The clearance of ¹⁸F-MyoZone from liver was very fast. The heart/liver ratios were 332, 5.31, 6.09 and 5.76 at 5, 30, 60 and 120 min post-injection, respectively. From 5 to 120 min post-injection, the outline of heart was clear and intact. There was almost no interference from the adjacent organs. The quality of PET images was highly satisfactory. Conclusions: ¹⁸F-MyoZone has the potential to be a good myocardial perfusion agent. The amount of K₂C0₃ used could significantly affect the labeling yield of ¹⁸F-MyoZone. (authors)