[en] Highlights: • The first time to investigate the mechanism of AGEs-associated impaired vasodilation. • AGEs impair endothelial-dependent but not endothelial-independent vasodilation. • AGEs inhibited eNOS mediated NO production and down-regulated eNOS expression. • Arginase overexpression by AGEs plays important role in the impaired vasodilation. • NADPH oxidase stimulation also mediates AGEs impaired vasodilation. Advanced glycation endproducts (AGEs) play a major role in the development of many vascular complications that are mediated by endothelial dysfunction. The present work aimed to investigate the mechanism by which AGEs impair vasodilation.