[en] Highlights: • A new approach to detect triketone HPPD inhibitors was set up via competition coordination. • The LODs for triketone HPPD inhibitors were nanomolar levels. • NMN was applied to detect mesotrione and tembotrione in water samples. 4-Hydroxyphenylpyruvatedioxygenase (HPPD) is a Fe(II)/Co(II)-dependent enzyme which has become one of the most effective herbicide targets. HPPD inhibitors have been developed as efficient herbicides for resistant weed control. Developing a method for efficient and rapid HPPD inhibitors detection is still challenging. N-n-butyl-4-methylhydrazinecarbothioamide-1,8-naphthalimide (NMN) was synthesized and used to detect Co²⁺ efficiently with the limit of detection (LOD) of 7.82 nM with a turn-on fluorescence. Herein a novel fluorescent complex, NMN‒Co²⁺ was employed to determine HPPD inhibitors which performed a turn-off effect in the sensing process based on the competitive coordination between the probe and HPPD with Co²⁺. The LODs for three commercial triketone HPPD inhibitors (mesotrione, tembotrione and NTBC) were 6.60 nM, 7.37 nM and 10.22 nM with good sensitivity and selectivity. Furthermore, the present probe has potentials to quantitatively detect mesotrione and tembotrione in real samples.