[en] To explore the allelopathic effect of caprylic acid on Conyza canadensis, based on the proteomics data of C. canadensis under caprylic acid treatment, the complete cDNA sequence of CcLhca-Z6 gene was cloned by homologous cloning and RACE technology. The expression of CcLhca-Z6 gene was measured by fluorescence quantitative PCR at different time after caprylic acid application. The results showed that the full length of CcLhca-Z6 was 1016 bp (Gene Bank NO. MH512007.1), encoding 266 amino acid residues with a molecular mass of 28.14 kD and theoretical pI of 5.29. Phylogenetic analysis showed that CcLhca-Z6 protein was clustered into a group with Artemisia annua (PWA84283.1), and the CcLhca-Z6 protein had the closest genetic relationship with Artemisia annua (PWA84283.1). RT-qPCR results showed that the treatment with caprylic acid significantly affected the expression of Lhca-z6 gene in the leaves of C. canadensis which showed a trend of first increasing and then descending with time. It was speculated that the CcLhca-Z6 gene may be the key gene in response to caprylic acid stress. This study laid a foundation for further exploring the function mechanism of plant herbicide caprylic acid. (authors)