[en] Innovative therapies are required to stimulate the research in the field of development of brain tumor models in biomedical applications; it requires an exploration due to the complex integrity and peculiar nature of the blood–brain barrier. Therefore, from cell culture to taking to therapeutics or earlier diagnostics for clinical trials, a brain tumor model requires major focus and research. In this piece of research we have tried to stich the major events to be taken care before proceeding for clinical trials in case of brain tumors, we have hereby established xenografts glioblastoma–U87-MG in athymic mice brain through stereotactic surgery and evaluated both functionally and histologically the changes induced in the BBB and endothelial cells and monitored the disruption of BBB during the progression of tumor in the mice brain. Nucleolipids are promising drug delivery systems which allow in vivo tracking using molecular imaging techniques and theranostics agents, especially for brain tumors. Thus, we have evaluated nucleolipid for in vivo tracking by radio labeling with ^99mTc via stereotaxis in mice model. Single photon emission computed tomography imaging showed specific uptake (1.8% ID) 15 min p.i of DTPA-NL-DPU at the site of tumor implantation as compared to the other organs except for the liver and kidney showing prolonged uptake due to the lipophilic nature of lipidic conjugate (^99mTc DTPANL- DPU). The xenogratfts were also studied for their survival analysis; the study provides a powerful and reliable approach for evaluating experimental therapeutic efficacy in animal model

[en] The development of drug delivery nano-carriers is emerging as a promising therapeutic tool to transport anticancer agents to tumors. In this contribution, preparation of nanoparticles (NPs) highly loaded with cisplatin using the bio-inspired hybrid nucleoside-lipids has been reported. The construction of these NPs using a 'layer-by-layer' approach allows surface functionalization with polyethylene glycol and targeting moieties. Uridine moieties were pegylated for folic acid (FA) functionalization to render specificity for active targeting. The uridine moieties at the surface of the nanoparticle act as ligands for ^99mTc radiolabeling, whereas the lipid chains maintain the structure of the nanoparticle. In vitro, these hybrid NPs are stable and actively internalize in two different cell lines over expressing the folic acid receptor. In vivo scintigraphy shows that nucleo-lipid functionalized NPs notably improved the pharmacokinetic profile of cisplatin (enhanced blood circulation time) and accumulated in the tumor xenografted mice model. (authors)