[en] Aim of this work was to evaluate influence of two commercial surfactants and inoculum of selected bacteria on biodegradation of diesel fuel in different systems. Among alkyl polyethossilates (Brij family) and sorbitan derivates (Tween family) a first selection of surfactants was performed by estimation of Koc and Dafnia magna EC50 with molecular descriptor and QSAR model. Further experiments were conducted to evaluate soil sorption, biodegradability and toxicity. In the second part of the research, the effect of Brij 56, Tween 80 and selected bacteria addition on biodegradation of diesel fuel was studied in liquid cultures and in slurry and solid phase systems. The latter experiments were performed with diesel contaminated soil in bench scale slurry phase bioreactor and solid phase columns. Tween 80 addition increased the biodegradation rate of hydrocarbons both in liquid and in slurry phase systems. Regarding the effect of inoculum, no enhancement of biodegradation rate was observed neither in surfactant added nor in experiments without addition. On the contrary, in solid phase experiments, inoculum addition resulted in enhanced biodegradation compared to surfactant addition
Journal
Annali di Chimica (Rome); ISSN 0003-4592; 
; v. 9; p. 799-805
; v. 9; p. 799-805
[en] A biosensor based on Pseudomonas putida cells was utilized for Benzene analysis in air of an oil refinery. Biosensoristic approach was compared to gaschromatografic essay. We also developed bio sensing Benzene genetically modified Escherichia coli and tested them with refinery samples. Microbial biosensor were useful to determine air pollution.
[it]
Un biosensore basato su cellule di Pseudomonas putida e stato impiegato per l'analisi del benzene in atmosfera di un impianto industriale di raffinazione del petrolio. Il metodo biosensoristico e stato confrontato con analisi gascromatografiche. Sono stati inoltre sviluppati ceppi di Escherichia coli geneticamente modificati per metabolizzare il benzene. La ricerca ha concluso l'analisi di campioni provenienti dalla raffineria. I sensori microbici sono risultati di facile impiego e sufficientemente affidabili per valutare l'inquinamento atmosferico.Journal
Chimica e l'Industria; ISSN 0009-4315; ; v. 91(5); p. 114-117
; v. 91(5); p. 114-117
[en] The Bo.S.Co project (Bioremediation of contaminated soils by compost) aims at creating an innovative bioremediation technology ready-to-use and competitive in price. This technology use a particular kind of certified compost that optimizes cleaning processes. Compost, in fact, is a very rich matrix that can supply nutrients, used by the autochthonous microflora. In the present study compost was used to enhance diesel oil and PAHs degradation in two heavily contaminated soils; laboratory scale experiments were performed by preparing four soil-bio piles, under laboratory conditions chemical, microbiological and eco toxic parameters were analyzed at different times. Compost addition was effective in enhancing biodegradation of diesel oil compounds and simultaneous reduction of genotoxicity with respect to the control.
[it]
Il progetto Bo.S.Co (Bonifica Siti Contaminati tramite Compost) ha come finalita quella di sviluppare una tecnica di biorisanamento innovativa, facilmente applicabile e competitiva sul mercato, utilizzando un particolare compost di qualita certificata che ha la caratteristica di essere una matrice molto ricca di nutrienti disponibili per la microflora autoctona. Nel presente lavoro il compost e usato per migliorare la degradazione di IPA (Idrocarburi Policiclici Aromatici) e gasolio presenti in due terreni contaminati. E stata svolta una sperimentazione su scala di laboratorio allestendo quattro biopile, sono state svolte analisi chimiche, microbiologiche e tossicologiche a diversi tempi. Si e riscontrato che l'aggiunta del compost migliora la degradazione del gasolio e riduce la genotossicita rispetto al terreno in cui non e stato utilizzato il compost.Journal
RS. Rifiuti Solidi; ISSN 0394-5391; ; v. 23(2); p. 95-98
; v. 23(2); p. 95-98