[en] Quadrupole giant resonances (GQR) play a major role in characterizing dynamical properties of both stable and unstable nuclei. In this paper two topics related with GQR are discussed based on recent experimental findings at RIKEN; 1) clear observation of isovector GQR in a heavy ion charge-exchange reaction, (¹³C, ¹³N) on ⁶⁰Ni at 100 MeV/nucleon, and 2) E2 effective charges varying with isospin asymmetry as evidenced by the electric quadrupole moments of neutron rich B isotopes

[en] A rat hepatocyte cell line which accumulates free heparan sulfate (HS) chains in the nucleus was labeled with ³⁵SO₄^2- and the rate of appearance of [³⁵SO₄]HS in the nucleus was measured. [³⁵SO₄]HS began to accumulate in the nucleus 2 h after the addition of ³⁵SO₄^2- and reached a steady state level after 20 h. HS was lost from the nuclei of pre-labeled cells with a t_1/2 of 8 h. At both 37 degree C and 16 degree C exogenous [³⁵SO₄] heparan sulfate proteoglycan (HSPG) was taken up by the cells and converted to free chains and about 10 percent of the internalized HS was transported into nucleus. The core protein of newly secreted HSPG appears in the cell matrix covalently linked to a phosphatidylinositol moiety on plasma membrane which is then cleaved by a plasma membrane phospholipase C. The released HSPG becomes found to a inositol-PO₄ (IP)-specific receptor and is internalized and a portion of it is processed by a non-lysosomal pathway before delivery to the nucleus. Insulin activates the activity of the plasma membrane phospholipase C

[en] Short communication. 3 refs

[en] Intense radioactive beams of projectile fragments have become available at RIKEN for studies on exotic nuclei. Experiments have been made along three major directions: (1) Study of the unique properties of neutron-halo nuclei using RI-beam secondary reactions. (2) Measurement of unstable nucleus reaction rates of astrophysical interest. (3) Production and application of spin-polarized RI-beams. Several reaction mechanisms characteristic of intermediate energy heavy ion collisions have been essential to facilitate these studies. Such useful mechanisms and, in particular, the spin-polarization mechanism in projectile fragmentation reactions will be discussed

[en] The proceedings includes 47 papers dealing with studies on heavy ion collisions in five different categories. The first part entitled EXOTIC NUCLEI AND RI BEAMS covers recent results obtained with new techniques of producing nuclei far from the stability line. Various studies of highly excited nuclei are presented in the HOT NUCLEI chapter. Recent development of studies on the HOT AND COLD GIANT RESONANCES excited by heavy ion collisions are discussed. In the chapter HIGH SPIN AND SOME APPLICATIONS, current topics on the high spin states are presented together with some interdisciplinary works. The PANEL FOR FUTURE COLLABORATION is intended to inspire people for new international co-operation of experimental and theoretical studies among different institutes. Present status or future plans of seven facilities of France, China and Japan are reported. 47 items are indexed separately for the INIS database. (K.A.)

[en] A new polarization phenomenon observed for projectile fragments in high-energy heavy ion collisions is reported. Polarization was as large as about 20% at maximum and exhibited a systematic dependence on the momentum of the fragment. The behavior of the polarization is interpreted in terms of a simple kinematical argument. The prospect of producing polarized RI beam for practical applications is also discussed

[en] A rat hepatocyte cell line was shown previously to contain a nuclear pool of free heparan SO₄ (HS) chains which turns over rapidly. These cells were labeled with ³⁵SO₄^2- and the steady state levels of [³⁵SO₄]HS in the nucleus were measured under culture conditions chosen to give different cell doubling times (DT's). In each log phase culture growing at a constant rate, the level of nuclear HS did not change as the cell density increased. Rapidly dividing cells had levels of nuclear HS within the range of 30-42 pmol ³⁵SO₄ in the nuclear HS/10⁶ cells. However, in cultures with decreased DT's, there was a corresponding decrease in the level of nuclear HS below 30 pmol/10⁶ cells that was proportional to the decrease in the DT. In contrast, when the cell DT increased in cultures approaching confluence, there was a corresponding increase in the level of nuclear HS above 42 pmols/10⁶ cells. The level of nuclear HS in cells which showed constant inhibition was maximal at 65-90 pmol ³⁵SO₄ in HS/10⁶ cells. Serum, insulin, hydrocortisone, and retinoic acid increased the amount of nuclear HS in log phase cells, whereas phorbol-12-myristate-13-acetate, diBucAMP, and decreased levels of inositol or choline lowered the amount. The correlation between DT's and nuclear HS levels suggest a role for nuclear HS in the regulation of cell division

[en] A rat hepatocyte cell line which accumulates free heparan sulfate (HS) chains enriched in GlcA-2-SO₄ residues in the nucleus was labeled with ³⁵SO₄^2- and the rate of appearance of [³⁵SO₄]HS in the nucleus was measured. [³⁵SO₄]HS began to accumulate in the nucleus 2 h after the addition of ³⁵SO^42- and reached a steady state level after 20 h. HS was lost from the nuclei of prelabeled cells with a t/sub 1/2/ of 8 h. Chloroquine did not inhibit the transport of HS into the nucleus, but increased the t/sub 1/2/ for the exit of HS from the nucleus to 20 h. At both 37⁰C and 16⁰C exogenous [³⁵SO₄]proteoHS was taken up by the cells and converted to free chains and about 10% of the internalized [³⁵SO₄]HS was transported into the nucleus. The [³⁵SO₄]HS isolated from the nucleus was enriched in GlcA-2-SO₄ residues, whereas the [³⁵SO₄]HS remaining in the rest of the intra-cellular pool showed a corresponding depletion in GlcA-2-SO₄ residues. The results show that nuclear HS is derived from the pool of a secreted proteoHS and that metabolism of exogenous HS by hepatocytes does not involve lysosomal processing of the internalized HS

[en] A new intermediate-energy heavy-ion accelerator, the RIKEN ring cyclotron, has recently started operation. A variety of ions with energies up to 42 MeV/u are presently available, while another year may be needed before the maximum energy of 135 MeV/u will be reached. In this talk, the author reports on some of the first experiments made with the primary beams. They are (1) Measurement of reaction cross sections using beams of stable and unstable nuclei, (2) Study of spin orientation of projectile fragments, and (3) Study of pion and hard-photon emissions. Aspects of spectroscopic applications of projectile-fragmentation processes as well as characteristic features of heavy ion collisions in the region of 40 MeV/u will be discussed. Related experimental apparatus under construction will also be described

[en] Previous studies showed that as monolayer cultures of a rat hepatocyte cell line passed from log growth to confluency there was an increase in sulfation of heparan sulfate (HS) and the accumulation of a unique species of HS with a high content of sulfated GlcA residues in the nucleus. The present study compares the HS metabolism of a rat (Morris) hepatoma line. Cells were labeled with ³⁵SO₄^2- and the structure and distribution of [³⁵SO₄]HS from the culture medium (CM), the pericellular matrix (Ma), the nucleus (NUC), the outer nuclear membrane (NM), and the remaining cytoplasmic (CP) pool was measured as nitrous acid-susceptible material. The amount of label incorporated into each pool was 1/10 that observed in the hepatocyte line. The HS proteglycan and the free HS chains from the hepatoma showed size distributions similar to those found for the hepatocytes, but a lower average charge density. In the HS from the CM, Ma, and CP pools 56% of glucosamine residues were sulfated; in that from the NM and NUC pools 46% were sulfated. HONO treatment gave mono- and disulfated disaccharides in a ratio of 1.5:1 for all five cellular pools, but showed that the HS from the NUC pool did not contain high levels of sulfated GlcA residues