[en] The principles are reported of the radioimmunoanalytical assay of the hepatitis B surface antigen, antibodies against this antigen which constitute immunologically indirect evidence, and antibodies against the nucleus of Dane's particles, which is circumstantial immunological evidence. The results obtained by radioimmunoassay are compared with those obtained by enzyme immunoassay. The results are presented obtained during the investigations of a total of 79 individuals, blood donors, health workers, and haemodialytic patients. In the whole group the hepatitis B surface antigen was proved by radioimmunoassay in 54%, by enzyme immunoassay in 47%; the antibody against the hepatitis B surface antigen in 19%; the antibody against the nucleus of the hepatitis B virus showed the largest proportion 75%. In 6.3% radioimmunoassay showed symptoms all three of hepatitis B, i.e., the surface antigen, the antibody against it, and the antibody against the hepatitis B virus nucleus; the correlation of the three symptoms is shown. The presence of HBsAg, HBsAb, and HBcAb is believed to be a contraindication of blood taking for routine purposes; the disappearance of HBsAg for a longer time may justify the re-inclusion among blood donors; the presence of HBsAb and HBcAb does not preclude the preparation of the plasma from such blood for the production of a specific anti-HBs immunoglobulin. (author)

[en] In rats, Wistar strain, after i.v. injection of an emulsion of methyl palmitate (0.4 g methyl palmitate per animal), using 740 kBq l-¹⁴C-leucine, proteosynthesis in liver and spleen homogenates and trichloroacetate precipitates was investigated. After 2-hour and 24-hour intervals following methyl palmitate administration an insignificantly raised leucine incorporation was observed in the liver and spleen. In the liver it can be explained by increased formation of enzymes needed for more intensive metabolic processes associated with the breakdown of palmitate. In the spleen it could be due to a more rapid replication of lymphocytes or enhanced formation of specific proteins, i.e., immunoglobulins. The return of these values to normal during subsequent intervals suggests, however, that only temporary changes are involved. (author)

[en] A description is presented of the radioimmunoassay of hepatitis B antigen e using the Abbott-HBe kit. The assay is based on what is known as a sandwich which consists of the human antibody against antigen e of hepatitis B, antigen e present in the sample of the serum examined, and an antibody labelled with ¹²⁵I. The assay based on the use of a gamma counter proceeds from the assumption that the higher the rate of pulses in the serum under investigation, the larger the quantity of the antigen in the serum. The report is given on the rate of antigen e occurrence in 94 individuals. The highest rate was recorded in patients hospitalized for hepatitis B (72%) and in the medical and nursing staff of the infectious hepatitis ward (39%). Antigen e was determined in 10% symptom-free carriers of hepatitis B surface antigen (HBsAg). The differences are listed in the time-dependent rate of occurrence and the quantity of antigen e and the surface antigen, as are present-day ideas of antigen e in the virus of hepatitis B, and the clinical significance of determining this particular antigen. The results are summed up in table. (author)

[en] The principle and procedure are described of the radioimmunoassay of delta antibody (delta-Ab) using the ABBOTT ANTI-DELTA kit by Abbott Co. A description is given of the kit, the working procedure and the method of evaluation. The results are reported of the incidence of delta-Ab in sera of patients with viral hepatitis B, in haemophiliacs, carriers of the hepatitis B virus surface antigen (HBsAg) and blood donors. The presence was detected of delta-Ab in one HBsAg carrier. The necessity is emphasized of delta-Ab determinations in the blood of donors in view of the antibody transfer with blood and blood preparations

[en] The radioimmunological detection of antibodies against hepatitis-A virus was performed in convalescents after hepatitis A, blood donors and in immunoglobulins made of convalescent plasma. The antibody was detected in nearly all patients after 2 months as well as one year after the termination of clinical treatment. The antibody titer in immunoglobulins made of the plasma of convalescents was convincingly higher than the titer in normal immunoglobulins. (author)

No abstract available

No abstract available

[en] The surface antigen of hepatitis B (HBsAg) and the antibodies against HBsAg can be determined separately by the radioimmunoassay (RIA) test kits AUSRIA II-125 and AUSAB, respectively. Using the test kit RIA Quick (IMMUNO, Wien) it is possible to perform both the determinations in one preparation simultaneously. The sensitivity of RIA Quick for HBsAg corresponds to that of AUSRIA II-125 and for HBsAB it is considerably lower than that of AUSAB. RIA Quick is of excellent technical design and is by a quarter cheaper than the kits for isolated determination of HBsAg and HBsAb

[en] The sensitivity is compared of determination of surface antigen HBsAg and nuclear antibody HBcAb of the hepatitis B virus using kits for separate (AUSRIA II-125, CORAB) and simultaneous (AUSRIAsup(R)/CORE) determinations of third generation tests in selected samples of medical personnel, HBsAg carriers, patients at a dialysis centre, blood donors and in sera of HBsAg carriers diluted in steps from 4x10^-2 to 4x10^-7. HBsAg is always determined using the RIA technique, HBcAb is determined using the technique of radioimmunoassay with the CORAB kit and with the AUSRIA sup(R)/CORE kit using enzymeimmunoassay. The sensitivity of determination using the AUSRIA sup(R)/CORE kit is at least as good for both investigated indicators of the hepatitis B virus and that obtained using separate determination of HBsAg (AUSRIA II-125) and HBcAb (CORAB), this also usino. modified photocolorimetric determination. Only one AUSRIA/sup R//CORE kit was available for the investigation and the informative character of the report is emphasized. (author)

[en] With regard to the high sensitivity of HBsAg and anti-HBs detection using kits HBsAg RIA QUICK and Anti-HBs RIA QUICK, and with regard to the excellent technical set-up which substantially reduces demands on manual work and eliminates the need to equip the department with a rinser, and also with regard to the lower price as compared with similar kits by Abbott Co., the above mentioned two kits will be imported to Czechoslovakia. (author)