[en] A study was undertaken to compare two new methods of capillary blood flow measurement, namely fluorescein flowmetry (FF) and laser Doppler flowmetry (LDF). The blood flow was measured in a pelvis pouch during its construction and in the completed ileoanal anastomosis in 12 patients. There was a high correlation between the two methods when the blood flow was measured in the pelvic pouch. The correlation coefficient between the two methods for the difference between the blood flow in the pelvic pouch at the site of the planned anastomosis when the pouch resided in the abdomen and that in the completed ileoanal anastomosis was r=0.99, the reduction amounted to 25% as measured by FF and 27% as measured by LDF. All ileoanal anastomoses healed perfectly, the lowest FF and LDF values being 0.004 density units/s and 0.3 V, respectively. The results indicate that either method can be considered for measuring capillary blood flow. 8 refs., 2 figs

[en] The extent of oxidoreduction of the 3 alpha-, 7 alpha- and 12 alpha-hydroxyl groups in bile acids during the enterohepatic circulation in man was studied with the use of [3 beta-³H]-labeled deoxycholic acid and cholic acid, [7 beta-³H]-labeled cholic acid, and [12 beta-³H]-labeled deoxycholic acid and cholic acid. Each [³H]-labeled bile acid was given per os to healthy volunteers, together with the corresponding [24-¹⁴C]-labeled bile acid. The rate of oxidoreduction was calculated from the decrease in the ratio between ³H and ¹⁴C in the respective bile acid isolated from duodenal contents collected at different time intervals after administration of the labeled bile acids. The mean fractional conversion rate was found to be 0.29 day-1 for the 3 alpha-hydroxyl group in deoxycholic acid (n = 2), 0.18 day-1 for the 12 alpha-hydroxyl group in deoxycholic acid (n = 6), 0.09 day-1 for the 3 alpha-hydroxyl group in cholic acid (n = 3), 0.05 day-1 for the 7 alpha-hydroxyl group in cholic acid (n = 2), and 0.03 day-1 for the 12 alpha-hydroxyl group in cholic acid (n = 2). The extent of oxidoreduction of the 12 alpha-hydroxyl group in [12 beta-³H]-labeled deoxycholic acid given to two patients operated with subtotal colectomy and ileostomy was markedly reduced (less than 20% of normal)