[en] Highlights: • The development of MDR bacteria and prevalence of BRIs have resulted in increased morbidity and mortality. • AMPs-based nano-formulation can be considered as the promising antimicrobial strategy in combating MDR bacteria and BRIs. • Nanotechnology promotes the clinical translation of AMPs as the next generation of antimicrobial agents. The rapid development of multidrug-resistant (MDR) bacteria due to the improper and overuse of antibiotics and the ineffective performance of antibiotics against the difficult-to-treat biofilm-related infections (BRIs) have urgently called for alternative antimicrobial agents and strategies in combating bacterial infections. Antimicrobial peptides (AMPs), owing to their compelling antimicrobial activity against MDR bacteria and BRIs without causing bacteria resistance, have attracted extensive attention in the research field. With the development of nanomaterial-based drug delivery strategies, AMPs-based nano-formulations have significantly improved the therapeutic effects of AMPs by ameliorating their hydrolytic stability, half-life in vivo, and solubility as well as reducing the cytotoxicity and hemolysis, etc. This review has comprehensively summarized the application AMPs-based nano-formulation in various bacterial infections models, including bloodstream infections (specifically sepsis), pulmonary infections, chronic wound infections, gastrointestinal infections, among others. The design of the nanomaterial-based drug delivery systems and the therapeutic effects of the AMPs-based nano-formulations in literature have been categorized and in details discussed. Overall, this review provides insights into the advantages and disadvantages of the current developed AMPs-based nano-formulations in literature for the treatment of bacterial infections, bringing inspirations and suggestions for their future design in the way towards clinical translation.

[en] Highlight• Both BPA and BPF interfered with TH signaling in Xenopus brains. • Both BPA and BPF affected T3-induced Xenopus brain development. • BPA and BPF exposure alone exerted weak effects on brain development. There is concern about adverse effects of thyroid hormone (TH) disrupting chemicals on TH-dependent brain development. Bisphenol A (BPA) and its analogues, such as bisphenol F (BPF), are known to have the potential to interfere with TH signaling, but whether they affect TH-dependent brain development is not yet well documented. Here, we conducted the T3-induced Xenopus laevis metamorphosis assay, a model for studying TH signaling disruption, to investigate the effects of BPA and BPF (10–1000 nM) on TH signaling in brains and subsequent brain development. While 48-hr treatment with 1 nM T3 dramatically upregulated TH-response gene expression in X. laevis brains at stage 52, 1000 and/or 100 nM BPA also caused significant transcriptional up-regulation of certain TH-response genes, whereas BPF had slighter effects, suggesting limited TH signaling disrupting activity of BPF in brains relative to BPA at the lack of TH. In the presence of 1 nM T3, 1000 and/or 100 nM of BPF as well as BPA antagonized T3-induced TH-response gene expression, whereas lower concentrations agonized T3 actions on certain TH-response genes, displaying an apparently biphasic effect on TH signaling. After 96 h exposure, T3 induced brain morphological remodeling coupled with cell proliferation and neuronal differentiation, whereas both BPA and BPF generally antagonized T3-induced changes in a concentration-dependent manner, with weak or no effects of bisphenol exposure alone. Overall, all results show that BPA and BPF interfered with TH signaling in Xenopus brains, especially in the presence of TH, and subsequently affected TH-dependent brain development. Given the evolutionary conservation of TH-dependent brain development among vertebrates, our findings from X. laevis warrant further studies to reveal potential influences of bisphenols on TH-dependent brain development in higher vertebrates.