[en] Objective: To investigate the mechanism of radiosensitizing effects of endostatin on H-520 human lung squamous cancer cells. Methods: H-520 cells was treated with endostatin and/or radiation. Colony-forming assays were used to indicate the radiosensitising effects. Cell cycle distribution and expression of phosphor-p38-MAPK were assayed by FCM, and cyclin D1, cdk2, cdk4 and survivin mRNA levels were assayed by RT-PCR, Phosphor-Akt was evaluated by Western-blotting. Results: Combination of endostatin and irradiation inhibited the proliferation of H-520 cells. According to the colony-forming assays, the D₀, D_q, D₁₀ and SF₂ values of the combination groups were lower than those of irradiation groups. The sensitization enhancement ratio (SER) was 1.51 G₂/M arrest occurred after 4 Gy irradiation. The gene expression of cyclin D1, cdk2, ckd4 and survivin and phosphor-Akt protein were down-regulated after treatment. The expression phosphor-p38-MAPK protein was also down-regulated after treatment with 200 μg/ml endostar. Conclusions: Endostatin inhibits the growth of H-520 cells and radiosensitizes the cells by induction of G₀/G₁ arrest, cell apoptosis and down-regulation of gene expression of cyclin D1, cdk2, cdk4 and reduces the phosphorylation of Akt and p38-MAPK. (authors)

[en] Objective: To retrospectively study the efficacy and side-effect of ¹²⁵I seed implantation combined with endocrinal therapy in stage T₃N₀M₀ prostate cancer. Methods: The study included 22 patients with clinical stage T₃N₀M₀ prostate cancer who were treated with transperineal ¹²⁵I seed implantation guided by transrectal ultrasound, real time TPS and endocrinal therapy. The minimum peripheral doses (MPD) were 140-160 Gy. The median number of seeds was 74(26-90). The activity of each seed was 1.55 × 10⁷ (1.30 × 10⁷-1.85 × 10⁷) Bq. 11 patients were treated with orchidectomy, and 11 patients were treated with androgen deprivation therapy. Results: All 22 patients completed the seed implantation successfully. The 5-year biochemical progression-free survival was 70.6%, and 5-year overall survival was 81.8%. 2 patients were found biochemical failure in 12 months after seed implantation, and another 1 patient failed in 90 months. Endocrinal therapy was followed thereafter. After the seed implantation, the urinary complications of grade 1 and 2 were 54.5% and 9.1% respectively, and the rectum side-effect of grade 1 and 2 were 22.7% and 9.1%.1 patient suffered rectal complication of grade 4. Conclusions: Good effect and tolerance are observed in prostate cancer patients of stage T₃N₀M₀ receiving ¹²⁵I seed implantation plus endocrinal therapy.The treatment can be considered for those who refuse to receive external beam radiotherapy. (authors)

[en] Phosphomolybdic acid zirconium loaded on silocone (ZrMoPP-SiO₂) adsorbent was synthesized by two-step method. The characterizations of the adsorbents were investigated by XRF, SEM, TEM, XRD, BET and XPS methods. The effects of both competitive ions and acidity on cesium adsorption of ZrMoPP-SiO₂ were also studied. The adsorption follows a typical Langmuir model and a pseudo second-order kinetic model. After adsorption of cesium, there is cesium peak on XPS of ZrMoPP-SiO₂, which means ZrMoPP-SiO₂ has great adsorption of cesium. Five co-existing cations and the change of acidity don't affect adsorption ability of ZrMoPP-SiO₂. Results show that ZrMoPP-SiO₂ has certain industrial application prospect. (authors)

[en] Objective: To investigate the effects of microRNA-17-92 on radiosensitivity of human mantle cell lymphoma cells. Methods: Tetracycline-regulated pRevTet-On expression system was established to generate cell line Z138c-miR-17-92 with over-expressed miR-17-92 and cell line Z138c-TMP2. Cell proliferation was measured by ³H-TdR incorporation and viable cell counting stained with typan blue. Cell cycle distribution was analysed by flow cytometry (FCM). Results: More viable and proliferous cells were counted in group miR-17-92, when exposure dose was greater than 2 Gy and incubation time was longer than 48 h under the same condition (t=-3.12 and -3.28, P<0.05). The percentage of G₂/M cell in group TMP2 was increased while no obvious cell cycle arrests were found in group miR-17-92 at 2 and 4 Gy (t=2.885, P<0.05). When cells were incubated for 96 h, higher percentage of propidium iodide (PI) positively stained cells were found in group TMP2 (24.02% vs. 36.16%) compared with group miR-17-92 (6.49% vs. 11.39%) at 2 and 4 Gy, respectively (t=-17.59, -4.972, P<0.05). Conclusions: Overexpression of microRNA-17-92 decreased the radiosensitivity of human mantle cell lymphoma cells by inhibition of cell cycle changes and cell apoptosis. (authors)

[en] Objective: To summarize the efficacy and the feasibility of ¹²⁵I seed implantation for recurrence cervical lymph node of head and neck tumor after radiotherapy or radiotherapy plus neck dissection. Methods: Thirty-six patients with the recurrence cervical lymphnode of head and neck tumor after radiotherapy (17 patients) or radiotherapy plus neck dissection (19 patients) were treated with ¹²⁵I seed implantation guided by ultrasound or CT under local anesthesia. The median number of seeds was 27( range from 3 to 78). Postoperative quality evaluation were routinely obtained for all patients. The actuarial D₉₀ ranged from 90-160 Gy (median, 130 Gy). Results: The follow-up rate was 100%. The number of the patients who were followed up over 1-and 2-year were 11 and 3. The overall response rate was 81%. The 1-and 2-year over local control rates, over survival rates were 69% and 35%, 50% and 22%, respectively. The 1-and 2-year local control rates in patients with recurrence node after radiotherapy plus neck dissection were 72% and 54%, while those were 67% and 50% in patients with recurrence node after radiotherapy,respectively (χ²=00, P=0.965). The 1-and 2-year survival rates in two groups were 48%, 13% , and 51%, 39%, respectively (χ²=0.17, P=0.676). Conclusions: ¹²⁵I seed implantation is a safe,minimal invasive with low morbidity and high efficacy salvage treatment method for cervical lymph node recurrence of head and neck tumor after radiotherapy with or without neck dissection. (authors)

[en] Objective: To compare the differences of gross tumor volume (GTV) and dose distribution between MRI-CT fusion imaging and CT-based imaging and investigate the dose difference in the therapeutic regime. Methods: Ten patients diagnosed with primary tumors of the cervical vertebra between 2013 and 2014 were enrolled. Prior to radiotherapy, the imaging data of MRI examination (GE Discovery MR 7503.0T) were collected, transfered into the Eclipse system and subject to fusion with CT images. GTV delineation, organ at risk (OAR) delineation and dose distribution discrepancy induced by target volume differences were analyzed and statistically compared between the MRI-CT fusion and CT-based images. GTV delineation and dose parameters among different radiologists between two approches were analyzed by analysis of variance (ANOVA) and paired t-test. Non-normally distributed variables were analyzed by Wilcoxon rank-sum test. The reliability of intraclass correlation coefficient (ICC) was assessed. Results: The GTV_MRI-CT volume was larger compared with the GTV_CT volume. The volume overlap index was 0.84 ± 0.17. The cord_MRI-CT volume was significantly less than the cordCT volume (P = 0.001). For 5 radiologists, the ICC_MRI-CT was larger than ICC_CT. The D_maxMRI-CT of the spinal cord was (46.00 ± 1.09) Gy, significantly less than (52.39 ± 1.34) Gy for the D_maxCT (P = 0.014). Conclusions: It is unlikely to miss the target delineation on MRI-CT fusion imaging. MRI-CT fusion imaging can minimize the discrepancy of interobserver radiologists and cause dosimetric advantages. (authors)

[en] Objective: To investigate the mechanism of radiosensitization by cetuximab (C225) on human tongue cancer Tca8113 cell line in vitro. Methods: Tca8113 cell line with and without C225 treatment received 6 MV X-ray irradiation of different doses (0, 2, 4, 6, 8 and 10 Gy). Cell proliferation, cell-cycle distribution and clonogenic survival were analyzed through cell counting, MTT, colony formation assay, and flow cytometry, respectively. Results: After irradiation of different doses, the growth inhibition rates in C225 group were higher than control (t =-15.6 - -3.0, P<0.05), the radiobiological parameters (D₀, D_q, N, and SF₂) in C225 group were lower than control so that SER of C225 group was 1.353, and the proportions of G₀/G₁ cells in C225 group were higher than control (t=-7.64, -7.89, -4.78, P<0.05) at 4, 6, 8 Gy. When the irradiation doses increased, the early phase apoptosis in both groups increased at first and then decreased with the maximum difference at 4 Gy [(7.96±0.36)% in C225 group and (4.13 ±0.29)% in control group, t=-12.75, P<0.01]. Conclusions: C225 has radiosensitization effect on Tca8113 cell line, possible through G₀/G₁ arrest and induction of apoptosis. (authors)

[en] In this paper, we report on the fabrication and characterization of poly(sulfone amide)/graphene (PSA/G) nonwoven based nanocomposite mat assembled via electrospinning technique. Different types of nanocomposite mats were electrospun by varying the weight percentage of graphene in the polymer solution. The surface morphologies, chemical structural, thermal, and electrical properties of the nanocomposites were evaluated systematically. The morphology of the PSA/G nanocomposites exhibited that mesh-like ultrafine nanofibers were densely aligned. Thermal stability and electrical properties of the PSA/G composites could be improved obviously with the addition of graphene. And the thickness uniformity of the nanocomposite mat was improved by using an electrospinning system. Our experimental results suggested that the PSA/G nanocomposites have potential to serve in many different applications, especially in the area of electronic components.

[en] Objective: To investigate the effect of Halo-Vest on the dose distribution of different radiotherapy techniques for primary cervical spine malignant tumors. Methods: Ten patients with primary cervical spine malignancies who underwent radiotherapy after Halo-Vest surgery were retrospectively studied. The IMRT and VMAT plans were designed on the contoured CT images including Halo-Vest delineations using Monaco planning system. The IMRT and VMAT plans with the same field parameters were duplicated to the CT images without the Halo-Vest delineations, and the dose distribution was recalculated. The dose distribution of the target, organs at risk and normal tissues was analyzed and compared for the plans with and without the Halo-Vest delineation. Results: For most dosimetric parameters of VMAT plans, the mean deviations induced by the Halo-Vest were less than 1%, except for PGTV_107%. Without Halo-Vest delineation, the mean maximum dose of spinal cord and spinal cord-PRV increased by 0.38 Gy and 0.42 Gy (Z = -2.803, -2.803, P < 0.05), respectively. The mean D_mean of spinal cord and spinal cord PRV increased by 0.35 Gy and 0.37 Gy, respectively (Z = -2.703, -2.701, P < 0.05). The maximum deviation observed in the mean V₅, V₃₀, and D_mean of mucosa, thyroid, parotid gland, mandible, mandibular joint, and normal tissues was 0.74%. For IMRT plans, larger dosimetric deviations than VMAT plans were observed in PTV and PGTV, most of which were more than 1.0% and the maximum deviation was 4.55%. The absence of Halo-Vest delineation increased the mean maximum dose of spinal cord and spinal cord-PRV by 0.48 Gy and 0.59 Gy (P > 0.05), respectively. The mean D_mean of spinal cord and spinal cord PRV increased by 0.57 Gy and 0.59 Gy, respectively (Z = -2.293, -2.293, P < 0.05). The maximum deviation of other organs at risk was 1.98%. Conclusions: There are no clinically significant dose differences for VMAT planning with or without Halo-Vest delineation on the CT images. But the dosimetric impact of absent or partial Halo-Vest delineation on IMRT planning is relatively large and should be considered. (authors)

[en] In order to improve the protein content of desert plant, a method of genetic transformation mediated by corona discharge was established. Artemisia seeds were processed in corona electric field for 120 min at 12 kV, and then soaked in 0.1 SSC media that contained Soybean Gy3 gene DNA to incubate for 12 h at 26 °C. Finally the seeds were inoculated on the differentiation medium. Polymerase Chain Reaction (PCR) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) detection showed that the Soybean Gy3 gene had been successfully introduced into genomic DNA of the regenerated plants of Artemisaarenaria. The study provided a new way for corona discharge in plant genetic modification.