[en] Highlights: • Around 40 photovoltaic and wind farms have been proposed in Yucatan, Mexico. • This region is recognized by its biodiversity richness and vulnerable ecosystems. • However, 19 Environmental Impact Assessment processes have been already authorized. • Environmental controversies are analyzed for 5 wind farms on the Yucatan coast. • This analysis would help to reduce environmental impacts and social discontent. Through the analysis of the corresponding Environmental Impact Assessment (EIA) for five wind projects set up on the Yucatan coast, in Mexico, this work seeks to draw attention to the environmental controversies that these projects generate. To carry out our analysis, we resorted to three sources of information that are part of the EIA process: (i) the Environmental Impact Statement (EIS), presented by private companies, for these wind farms; (ii) observations and criticisms of the EIS, presented by the civil society during the public process to which the EIS is submitted; and (iii) the rulings emitted by the federal environmental authorities. This documentation was complemented by participation in a number of forums and some interviews. Based on this analysis, we defined a series of deficiencies in the EIA process. Accordingly, we can affirm that in Mexico, as in other countries, there is no wind energy plan capable of reconciling energy objectives with nature conservation. Renewable energy projects in Mexico have been designed solely based on the distribution of wind or solar resources, completely ignoring environmental or social issues. In this regard, a sustainable energy transition requires strengthening the environmental management capabilities of governments, and the application of mechanisms that will allow the integration of environmental variables in the early stages of decision making, using instruments such as the Strategic Environmental Assessment.

[en] Research highlights: → The reaction catalyzed by one enzyme of the pentose phosphate pathway was studied. → A spectrophotometric method is proposed for kinetic and thermodynamic analysis. → The pH and the temperature influences are reported on physical chemical properties. → Relative concentrations of substrates are also important in the catalytic process. - Abstract: The enzyme glucose-6-phosphate dehydrogenase (G6PD, EC 1.1.1.49) from Leuconostoc mesenteroides has a dual coenzyme specificity with oxidized nicotinamide adenine dinucleotide (NAD_ox) and oxidized nicotinamide adenine dinucleotide phosphate as electron acceptors. The G6PD coenzyme selection is determined by the metabolic cellular prevailing conditions. In this study a kinetic and thermodynamic analysis is presented for the reaction catalyzed by G6PD from L. mesenteroides with NAD_ox as coenzyme in phosphate buffer. For this work, an in situ spectrophotometric technique was employed based on the detection of one product of the reaction. Substrate and coenzyme concentrations as well as temperature and pH effects were evaluated. The apparent equilibrium constant, the Michaelis constant, and the turnover number were determined as a function of each experimental condition. The standard transformed Gibbs energy of reaction was determined from equilibrium constants at different initial conditions. For the product 6-phospho-D-glucono-1,5-lactone, a value of the standard Gibbs energy of formation is proposed, Δ_fG^o = -1784 ± 5 kJ mol^-1.