[en] Fenvalerate (FV) is widely used in aquaculture because of their broad spectrums and high efficiency. However, little is known regarding the elimination of FV influenced by environment factors in aquaculture water, especially its cis-trans isomers (cis-FV and trans-FV). In the present study, factors influencing the aquaculture environment (open type, temperature, pH and light) were selected, and the elimination dynamics of FV and its cis-trans isomers in aquaculture water using orthogonal experiments were investigated. The results showed that the half-life and elimination rate range of FV were 4.75–11.95 days and 65–93%, respectively, while those of trans-FV were 4.60–11.82 days and 67–93% and those of cis-FV were 4.94–12.04 days and 64–92%, respectively. The elimination rate of trans-FV was better than that of cis-FV. Additionally, analysis of variance (ANOVA) of the orthogonal experimental data indicated that the environmental factors of open type, temperature, and pH significantly influenced the elimination rate of cis- and trans-FV (P < 0.05), that is, in the aquaculture season, high temperature and pH facilitate to eliminate FV. This study would improve our understanding of natural degradation associated with FV and guide safe to use associated with pesticide in aquaculture.

[en] Water quenched slag particles (WQSP) and haydite were applied to treat municipal wastewater in two lab-scale up-flow biological aerated filters (BAF) to compare their abilities to act as biofilm supports. The results showed that WQSP reactor brought a relative superiority to haydite reactor in terms of chemical oxygen demand (CODcr) and ammonia nitrogen (NH₃-N) removal when hydraulic retention time (HRT) ranged from 1 h to 5 h.Compared with haydite, WQSP had higher total porosity, larger total surface area and lower bulk and apparent density. Tests of heavy metal elements in lixivium proved that WQSP were safe for wastewater treatment. In addition, the detection of the amount of hetero bacteria and nitrobacteria of two biological aerated filters in three HRTs also showed that WQSP medium was more suitable to the attached growth of nitrobacteria, which is helpful to the improvement of nitrification performance in WQSP BAF. Therefore, WQSP is a potential material for use as the filter media of BAF for wastewater treatment. WQSP application, as a novel process of treating wastes with waste, provides a promising way to use water quenched slag (WQS). -- Highlights: ► Novel filter media-water quenched slag particles (WQSP) were prepared. ► Two upflow BAFs were applied to treat municipal wastewater. ► WQSP reactor brought a relative superiority to haydite reactor. ► WQSP medium was more suitable to the attached growth of nitrobacteria. ► The application provided a promising way in water quenched slag waste material utilization.

[en] Highlights: • TA-MSN@Ag is prepared by Ag⁺ reduction and electrostatic reaction between TA and MSN. • Ca-TA-MSN@Ag with Janus structure is obtained by chelation of TA and Ca²⁺. • Ca-TA-MSN@Ag can activate the internal and external hemostatic pathway. • Excellent antibacterial activity is due to the synergistic effect of TA and Ag NPs. • The good biocompatibility of Ca-TA-MSN@Ag provides a prospect clinical application. It is important to control bleeding and prevent bacterial infection for the wound people. The effective way is to fabricate an asymmetric Janus matrial for realizing rapid hemostasis and promoting wound healing. Herein, mesoporous silica nanoparticles (MSN) modified by tannic acid (TA), silver nanoparticles, and calcium ions (Ca-TA-MSN@Ag) with Janus structure were prepared via redox and coordination reactions. These anisotropic snowman-like particles possess obvious chemical compartition, in which silver nanoparticles are embedding in large MSN body. During blood coagulation, TA with catechol structure acts as a vasoconstrictor. Then, Ca-TA-MSN@Ag with high specific surface area (510.62 m²·g⁻¹) and large pore volume (0.48 m³·g⁻¹) induces red blood cell aggregation to form three-dimensional network structure with fibrin. Additionally, calcium ions as clotting factor IV and negative charge of Ca-TA-MSN@Ag accelerate coagulation cascade reaction. These three synergistic effects on animal model showed that hemostatic time of Ca-TA-MSN@Ag was shortened by nearly 50% compared to that of MSN. Moreover, Ca-TA-MSN@Ag possessed good blood compatibility, biocompatibility and antibacterial activity (~99%) against E. coli and S. aureus. The anisotropic Janus particles of Ca-TA-MSN@Ag with hemostatic performance and antibacterial activity will be a promising biomaterial for designing wound dressings in clinical application.

[en] Highlights: ► Target IgE is successfully designed to serve as a barrier to separate enzyme from its substrate. ► This sensing platform of electronic channel switching-based aptasensor can be simply manipulated. ► The stable hairpin structure of anti-IgE aptamer is utilized to detect target IgE. ► The sensor is ultrasensitive sensitivity, excellent selectivity and small volume of sample. ► It is a powerful platform to be further expanded to detect more kinds of proteins and even cells. - Abstract: Due to the ubiquity and essential of the proteins in all living organisms, the identification and quantification of disease-specific proteins are particularly important. Because the conformational change of aptamer upon its target or probe/target/probe sandwich often is the primary prerequisite for the design of an electrochemical aptameric assay system, it is extremely difficult to construct the electrochemical aptasensor for protein assay because the corresponding aptamers cannot often meet the requirement. To circumvent the obstacles mentioned, an electronic channel switching-based (ECS) aptasensor for ultrasensitive protein detection is developed. The essential achievement made is that an innovative sensing concept is proposed: the hairpin structure of aptamer is designed to pull electroactive species toward electrode surface and makes the surface-immobilized IgE serve as a barrier that separates enzyme from its substrate. It seemingly ensures that the ECS aptasensor exhibits most excellent assay features, such as, a detection limit of 4.44 × 10⁻⁶ μg mL⁻¹ (22.7 fM, 220 zmol in 10-μL sample) (demonstrating a 5 orders of magnitude improvement in detection sensitivity compared with classical electronic aptasensors) and dynamic response range from 4.44 × 10⁻⁶ to 4.44 × 10⁻¹ μg mL⁻¹. We believe that the described sensing concept here might open a new avenue for the detection of proteins and other biomacromolecules.

[en] Highlights: • A novel phosphorus removal yeast BL3 was isolated and identified. • A plausible phosphorus transfer pathway was proposed. • EPS was a major phosphorus reservoir. • BL3 had outstanding tolerance to phosphorus concentration, temperature and pH. A novel phosphorus removal yeast BL3 was isolated from an alternating anaerobic/aerobic biofilter and identified as Diutina rugosa by 26S rDNA gene sequence analysis. Yeast BL3 could effectively remove phosphorus from synthetic wastewater containing 2–20 mg/L phosphorus under optimal environmental conditions. The highest phosphorus removal efficiency was above 70% under the conditions of DO 6.86 mg/L, C/P ratios of 60, N/P ratios of 3.3, pH 6.0–9.0, and at 25.0–35.0 °C. The phosphorus distribution in the aqueous solution and different components of yeast BL3 analysis indicated that around 55%–70% and 20%–40% of removed phosphorus were transferred into extracellular polymeric substances (EPS) and yeast cells, respectively. The plausible phosphorus transfer pathway was proposed based on the phosphorus distribution and species analysis, suggesting the important role of EPS as a phosphorus reservoir. These results indicate that yeast BL3 can efficiently remove phosphorus under aerobic conditions without alternating anaerobic/aerobic cycling, and thus has significant potential for practical application in wastewater phosphorus removal.

[en] Highlights: • Enroflxacin and ciprofloxacin were the main types of antibiotics used in aquaculture ponds for cultured crabs. • Dietary assessment showed that EDI of all samples accounted for less than 1.6% of the ADI. • There were almost no risk associated crab consumption in China. The occurrence of 27 antibiotics (18 sulfonamides and 9 quinolones) in 92 samples of cultured Chinese mitten crab (Eriocheir sinensis) from three provinces (Anhui, Jiangsu and Liaoning) was investigated. These 21 antibiotics were detected at least once in crabs from these provinces with detection frequencies of 3.70‐90.91%. Sulfonamides were detected in 53.7% of the samples at concentrations of 0.1–10 μg/kg in Jiangsu, while quinolones were detected with 90.9% of samples containing concentrations of 1–100 μg/kg in Liaoning province. Enroflxacin, ciprofloxacin, sulfaquinoxaline, sulfameter, sulfadoxine, and sulfamethoxazole were the mainly used antibiotics and enroflxacin were present at a high concentration (>100 μg/kg). Dietary assessments showed that residual antibiotics in crabs from China were far below the maximum residue limit (MRL) of total sulfonamides and quinolones, and there was almost no risk associated with crab consumption. These results will provide meaningful indications for the safety of crab consumption.