[en] Highlights: • Benzo(a)pyrene exposure in utero upregulated SNCA mRNA transcription. • Benzo(a)pyrene exposure in utero injured protein degradation system. • Benzo(a)pyrene exposure in utero promoted α-syn accumulation and aggregation. • Benzo(a)pyrene exposure induced microglial activation and neuroinflammation. • Benzo(a)pyrene exposure induced dopaminergic neuronal loss in the substantia nigra. Previous work indicated that benzo[a]pyrene (B(a)P) exposure in utero might adversely affect neurodevelopment and cause Parkinson's Disease (PD)-like symptoms. However, the effect of utero exposure to B(a)P on PD-like α-synucleinopathy and the mechanism under are unclear.