[en] Preparation of antibodies against testosterone is described. The produced antibody proved suitable for application in the radioimmunoassay of testosterone. The sensitivity of detection using this antibody was 50 pg. (authors)

[en] Rabbit and bovine serum immunoglobulins were prepared by precipitation with rivanol, salting-out with ammonium sulphate, and by gel filtration. These partly purified antigens were used for immunization of two goats and one pig by intradermal administration. The dynamics of the development of precipitating antibodies were evaluated by turbidimetry. This method of evaluating the dynamics of antibody proved superior to both radial immunodiffusion using incorporated antigen and electroimmunodiffusion. The method is rapid and therefore suitable for determination of the optimum time for blood collection. (authors)