[en] The field of therapeutic nuclear medicine is emerging rapidly as choice of treatment in oncology and other cellular malignancies. The growth of this branch of nuclear medicine is greatly facilitated by the introduction of a number of new radiopharmaceuticals and radionuclides. ⁹⁰Y-radiopharmaceuticals have confirmed their worth in medical and clinical areas in a very short span of time. The ⁹⁰Y is a radioisotope widely used for therapeutic purposes and considerable perfection has been made to understand the chemistry of ⁹⁰Y-labeled radiopharmaceuticals. The development of these radiopharmaceuticals can be made favorable by using appropriate buffer, incubation period, optimal pH, specific activity and reaction temperature. In this review, we have discussed the preparation of range of ⁹⁰Y transporting biological molecules such as antibodies radiolabeled peptides, antigens and microsphere with their clinical applications. (author)

[en] Mitomycin-C is used for the treatment of different types of tumours. In present work, a reliable method was developed for radiolabeling of Mitomycin-C with ^99mTc for diagnostic purpose. ^99mTc-Mitomycin-C was obtained with radiochemical yield of 100 % by adding 200 µg Mitomycin-C, 1 mL (15 mCi) of pertechnetate in 25 μg SnCl₂·2H₂O at pH 7. Labeling efficiency was determined by paper chromatography and ITLC. The charge on ^99mTc-Mitomycin-C was determined by electrophoresis technique. HPLC analyses were performed for the determination of purity of Mitomycin-C and radiochemical purity of labeled complex. Evaluation of ^99mTc-Mitomycin-C, in vitro stability, biodistribution and scintigraphic images in normal mice were performed. (author)

[en] In present study it is aimed to radiolabel vincristine with ^99mTc and to evaluate bioaffinity of ^99mTc labeled vinc. The optimum conditions required to obtain 99.6 ± 0.4 %, (n = 5) radiolabeling yield of ^99mTc-vincristine (^99mTc-vinc) were as follows: pH 4, 5 µg of vincristine sulphate, 6 µg SnCl₂·2H₂O as a reducing agent and 10 min incubation time at room temperature. Quality control of ^99mTc-vinc was done by using paper electrophoresis and thin layer chromatography. The radiolabeling yield was confirmed by High performance liquid chromatography using radioactive and UV detector operating at 230 nm. ^99mTc-vinc was stable in vitro for 5 h. Biodistribution and scintigraphy of ^99mTc-vinc was performed in mice and rabbits respectively and that ^99mTc-vinc showed high uptake of it in liver and spleen. Finally ^99mTc-vinc may be the potential imaging agent for liver and spleen. (author)