[en] Tomato spotted wilt virus (TSWV, Genus: Tospovirus, Family: Bunyaviridae) is a major constraint to the production of several different crops of agronomic and horticultural importance worldwide. The amino acid sequence of the two envelope membrane glycoproteins, designated as G_N (N-terminal) and G_C (C-terminal), of TSWV contain several tripeptide sequences, Asn-Xaa-Ser/Thr, suggesting that the proteins are N-glycosylated. In this study, the lectin-binding properties of the viral glycoproteins and their sensitivities to glycosidases were examined to obtain information on the nature of potential oligosaccharide moieties present on G_N and G_C. The viral proteins were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and probed by affinoblotting using a battery of biotinylated lectins with specificity to different oligosaccharide structures. G_C showed strong binding with five mannose-binding lectins, four N-acetyllactosamine-binding lectins and one fucose-binding lectin. G_N was resolved into two molecular masses and only the slow migrating form showed binding, albeit to a lesser extent than G_C, with three of the five mannose-binding lectins. The N-acetyllactosamine- and fucose-specific lectins did not bind to either molecular mass form of G_N. None of the galactose-, N-acetylgalactosamine-, or sialic acid-binding lectins tested showed binding specificity to G_C or G_N. Treatment of the denatured virions with endoglycosidase H and peptide:N-glycosidase F (PNGase F) resulted in a significant decrease in the binding of G_C to high mannose- and N-acetyllactosamine-specific lectins. However, no such differences in lectin binding were apparent with G_N. These results indicate the presence of N-linked oligosaccharides of high mannose- and complex-type on G_C and possibly high mannose-type on G_N. Differences in the extent of binding of the two envelope glycoproteins to different lectins suggest that G_C is likely to be more heavily N-glycosylated than G_N. No evidence was observed for the presence of O-linked oligosaccharides on G_N or G_C