[en] The V₂O₅ self-assembled nanosheets (SANs V₂O₅) were synthesized by an additive-free ultrasonic method with subsequent thermal decomposition. The loose nanostructured V₂O₅ is multi-layer structure stacked by 4∼6 layers with a thickness of 200∼300 nm and each layer is composed by abundant nanoparticles in order. Due to this unique layered structure, the SANs V₂O₅ exhibits remarkable rate performance and excellent cycling stability. Specifically, it delivers reversible discharge capacities of 157.2 and 111.6 mAh g⁻¹ at current densities of 2C and 5C. After 300 cycles, it still maintained high capacities of 123.5 and 85.1 mAh g⁻¹, which corresponds to a capacity loss of 0.07% and 0.08% per cycle, respectively. In addition, even at 10C, it displays a capacity of 68.8 mAh g⁻¹ and 87.9% of the capacity (60.5 mAh g⁻¹) is remained after 300 cycles. All the results indicate the V₂O₅ self-assembled nanosheets could be a promising candidate as cathode active material for long-term cycling performance in Lithium-ion batteries.