[en] Highlights: • Proposing aeroengine data reconciliation model. • Analyzing the moving track of cooperative working point on characteristic map. • Using Automatic differentiation to calculate Jacobian and Hessian matrixes. • Applying data reconciliation model to eliminate test errors in a ground-run. -- Abstract: Aeroengine measuring data usually contain random errors and gross errors. Meanwhile some parameters cannot be measured directly due to costs or technology limitations. To improve the accuracy of these data, this paper proposes a novel reconciliation model based on cooperative working equations and characteristic maps. The interior-point method is applied to solve the objective functions and constraint equations. Robust estimators are verified, and the moving track of the cooperative working points during the reconciliation process is analyzed. The results show that the errors of the measured and unmeasured data can be reduced through data reconciliation, and the proposed data reconciliation model is not sensitive to gross errors, thereby indicating satisfactory performance.

[en] Objective: To investigate the effects of microwave exposure on motor learning and Glutamate receptor 2(GluR2) phosphorylation in rat cerebellum. Methods: The rabbits were trained for seven days to form eye-blink conditioning, and then divided randomly into control and microwave exposure group (at hours 0,3,24 and 72 subgroups after exposure, respectively). The rabbits were accepted 90 mW/cm² microwave exposure for 30 minutes, and the rectal temperature were detected immediately after exposure and specific absorption rate (SAR) value were calculated. Eye-blink conditioning were detected immediately after exposure, and cerebellar GluR2 protein and GluR2 phosphorylation were detected with Western blotting. Results: Rectal temperature of rabbits were increased by 3.02 degree C after exposure, and SAR value was 8.74 W/kg. The eye-blink conditioning decreased significantly after exposure, and cerebellar GluR2 protein expression had no significant alteration but phosphorylation reduced significantly after exposure. Conclusions: 90 mW/cm² microwave exposure has injurious effects on cerebellar GluR2 phosphorylation and motor learning. (authors)

[en] Objective: To determine the activation of microglia and the phosphorylation of Jaks, the upstream factors of JAK/STAT(janus activated kinase/signal transducers and activators of transcription) signaling pathway, after electromagnetic irradiation. Methods: Rats were irradiated by 90 mW/cm² EMF for 20 min. The phosphorylation of Jaks was determined by western blot at different time after electromagnetic irradiation. The activation of microglia was determined by immuno- chemistry. Results: GSA-IB4 was upregulated in microglia, which indicated microglia was activated after electromagnetic irradiation. The phosphorylation of Jak1, Jak2 and Jak3 in rat hippocampus was upregulated after electromagnetic irradiation. The phosphorylation of Jakl was upregulated after microwave exposure and peaked at 12 h. Jak2 peaked at 0 h after electro-magnetic irradiation and sustained in a high level. Jak3 was slightly affected by electromagnetic irradiation. All the three members of JAKs return to normal at 72 h after electromagnetic irradiation. Conclusion: Microglia cells was activated after electromagnetic irradiation. The phosphorylation of Jaks was upregulated by electromagnetic irradiation. It suggested that JAK/ STAT singnaling pathway was activated after electromagnetic irradiation, which indicated that JAK/STAT signaling pathway may participate in brain microglia activation induced by electromagnetic irradiation. (authors)

[en] Objective: To study the differentially expressed gene profiles by intestinal epithelial cells (IECs) derived from mice with mild intestinal type of radiation sickness. Methods: Total RNA of IECs obtained from normal animals and mice at 3 h, 96 h after 12 Gy γ-ray irradiation was isolated by Chomzynski's method and mRNA was purified using magnetic bead method. High specific activity ³²P-cDNA probes (0.5 x 10⁶ cpm/μg) were made by a single round reverse transcription with 200 U of Moloney murine leukemia virus reverse transcriptase in the presence of CDS primer (Clontech Co). The ³²P-cDNA probes were hybridized to the microarry membrane which contains 588 genes. After scanning the hybridization signal by gel analysis system, the differentially expressed gene profiles of the IECs derived from mice at 3 h, 96 h after 12 Gy γ-ray irradiation were made. Results: The differentially expressed gene profiles showed that the γ-ray induced gene expression pattern at the early stage (3 h) by IECs represented mainly inhibition of gene expression. The results of the differential gene expression mainly represented the IECs damage and resistance to radiation damage. At the IECs regeneration stage (96 h after irradiation), the differentially expressed genes mainly exhibited increase of gene expression and appearance of new gene expression. Besides, cdk inhibitor I protein gene remained at a stably high level of expression during radiation injury. Conclusion: The differential gene expression at the regeneration stage after 12 Gy γ-ray irradiation of IECs results in epithelium recovery. Perhaps the cdk inhibitor I protein could be used to indicate the injury induced by radiation