[en] Highlights: • A probability prediction method of crack growth rate of EAC in Nickel-based alloys is proposed. • The deterministic result which is a definite value among random results, and it can't completely describe the law of crack growth rate of EAC. • The randomness of load has great influence on the uncertainty of the crack growth rate of EAC. -- Abstract: Environmentally assisted cracking (EAC) of nickel-based alloys is one of the most significant potential safety hazards in the primary circuit of nuclear power plants. Considering the uncertainties of load and material properties of base metal and the oxide film, Latin hypercube sampling method is applied to predict the EAC crack growth rate combining the film slip-dissolution/oxidation model with the elastic-plastic finite element method. Meanwhile, to improve the efficiency of numerical analysis, MATLAB is employed in the secondary development for ABAQUS. With the help of existing experimental EAC data, the probability prediction of the growth rate at the tip of EAC in one-inch compact tension specimen is simulated and analyzed in this study. The results show that the randomness of material properties and load markedly affect the uncertainty of strain and EAC growth rate. Among the variables, The randomness of the load has the greatest influence on the uncertainty of the crack growth rate of EAC.

[en] Highlights: • Porcine reproductive and respiratory syndrome (PRRS) affects industrial pig farming. • N protein is the main protein of PRRS virus (PRRSV) in infected cells which is important for PRRSV replication. • Moloney leukemia virus 10 protein (MOV10) is a new host restriction factor of PRRSV. • MOV10 interacts with PRRSV N protein and inhibits PRRSV replication in vitro. • MOV10 inhibits nuclear entry of N protein, thus impairing viral life cycle. Porcine reproductive and respiratory syndrome virus (PRRSV) has been a major threat to global industrial pig farming ever since its emergence in the late 1980s. Identification of sustainable and effective control measures against PRRSV transmission is a pressing problem. The nucleocapsid (N) protein of PRRSV is specifically localized in the cytoplasm and nucleus of virus-infected cells which is important for PRRSV replication. In the current study, a new host restricted factor, Moloney leukemia virus 10-like protein (MOV10), was identified as an inhibitor of PRRSV replication. N protein levels and viral replication were significantly reduced in Marc-145 cells stably overexpressing MOV10 compared with those in wild-type Marc-145 cells. Adsorption experiments revealed that MOV10 did not affect the attachment and internalization of PRRSV. Co-immunoprecipitation and immunofluorescence co-localization analyses showed that MOV10 interacted and co-localized with the PRRSV N protein in the cytoplasm. Notably, MOV10 affected the distribution of N protein in the cytoplasm and nucleus, leading to the retention of N protein in the former. Taken together, these findings demonstrate for the first time that MOV10 inhibits PRRSV replication by restricting the nuclear import of N protein. These observations have great implications for the development of anti-PRRSV drugs and provide new insight into the role of N protein in PRRSV biology.