[en] The optimum labelling condition was established for using a new bifunctional chelating agent (DOTA-Peptide) in ⁹⁰Y-labelled monoclonal antibody Lym-1 by the pre-labelling method, in which the ⁹⁰Y was firstly labelled to the bifunctional chelating agent and then conjugated to the monoclonal antibody. the results showed that the radiolabelling procedure was reasonable and the radioactivity yield in products was satisfactory. The radiochemical purity of ⁹⁰Y-labelled Lym-1 was determined to be over 95% by gel filtration HPLC and silica gel TLC. The immunoreactivity of the final product was found to be greater than 100% relative to ¹²⁵I-Lym-1 (as a standard) by in vitro cell binding assay

[en] The method of labelling DTPA-Ab (antiferretin antibody of human hepatocellular carcinoma) conjugate with ⁹⁰Y as well as the analytical method of the labelled compounds is presented. The labelling percentage of the DTPA-Ab conjugate separated by micro-centrifuged column gel filtration using Sephadex G-75 is as high as 92%. After further purification, the radiochemical purity can be higher than 99%. The experimental results also show that the dilution factor of DTPA-Ab conjugate and ⁹⁰Y as well as the presence of impurity metal ions have great influence on the ⁹⁰Y labelling of DTPA-Ab conjugate

[en] The separation of the radioactive components in ⁹⁹Tc-HMDP-NaBH₄ label system both by anion exchange HPLC and ion pair HPLC is described. It is discovered that there are at least four different ⁹⁹Tc compounds or compounds with different valency. The radioactivity of the main peak reaches about 90% of total radioactivity

[en] The most available positron radionuclide labelled radiopharmaceuticals for clinical diagnosis are the compounds labelled with ¹⁸F, ¹¹C, ¹⁵O and ¹³N which have short half-life. This review described the syntheses and clinical use of the short half-life positron radionuclide labelled radiopharmaceuticals, especially ¹⁸F and ¹¹C labelled compounds

[en] Solutions of ⁹⁹Tc-HMDP and ⁹⁹Tc-HEDP were prepared by reducing ⁹⁹TcO₄^- with excess SnCl₂ in presence of corresponding chelating agent. the quantity of unreacted SnCl₂ was analyzed with I₂ standard solution by potentiometric titration. it was found that ⁹⁹TcO₄^- is reduced to the +4 state in eigher ^9Tc-HMDP or ⁹⁹Tc-HEDP at pH∼3. The whole experiment was carried out under N₂ atmosphere. The UV spectrum of ⁹⁹Tc-HMDP or ⁹⁹Tc-HEDP shows that during the iodometric titration ⁹⁹Tc exists only in one oxidation state and, they are stable in air whithin 1h

[en] Background: Folate receptor is highly expressed in tumor cells. Labeling folic acid offers a way of tumor targeting. Purpose: This study aims to explore the synthesis and in vivo imaging of ¹⁸F labeled folic derivative [¹⁸F]FPTZFA. Method: Starting from folic acid, through the amino and carboxyl ester forming reaction, we get FA-N₃, and then FA-N₃ is labeled with ¹⁸F-Pykyne in the presence of CuSO₄, VcNa to get the compound of [¹⁸F]FPTZFA. [¹⁸F]FPTZFA then characterized by Radio-HPLC (High Performance Liquid Chromatography) and separated by Semi-HPLC. In vivo imaging was carried out in KB tumor-bearing nude mice after intravenous injection of [¹⁸F]FPTZFA via tail vein and biodistribution of [¹⁸F]FPTZFA was performed in these mice. Results: The overall reaction time lasts for 40 min, with radiochemical purity of 51%, and radiochemical yield of 37%. The autoradiography of mice tissues showed [¹⁸F]FPTZFA concentrated in liver, kidney and tumor. The %ID/g ratio in tumor tissue and muscle is 3.6, %ID/g ratio in tumor tissue and blood is 3.2. Conclusion: The high efficiency and high yield make the synthesis of [¹⁸F]FPTZFA more convenient and the %ID/g ratio indicates [¹⁸F]FPTZFA as a promising tumor targeting tracer. (authors)

[en] The purpose of this article is to study the preparation and biodistribution of ⁹⁹Tc^m-DTPA-folate in nude mice with a human tumor cell implant. DTPA-folate was synthesized by conjugating folic acid to diethylenetriaminepentaacetic (DTPA), then radiolabeled with ⁹⁹Tc^m. The radio-chemical purity and stability were evaluated. The biodistribution of ⁹⁹Tc^m-DTPA-folate in nude mice with a human tumor cell implant was studied. The result showed that the radiolabelling yield of ⁹⁹Tc^m-DTPA-folate was as high as 98%. After 4 h at room temperature, the radiochemical purity of ⁹⁹Tc^m-DTPA-folate was still over 90%. ⁹⁹Tc^m-DTPA-folate conjugation showed marked tumor-specific deposition in vivo. The tumor-to-muscle ratio at 8 h post intraperitoneal injection was 7.5. ⁹⁹Tc^m-DTPA-folate appears to be a potential radiopharmaceutical for targeting tumor-associated folate receptor. (authors)

[en] The purpose of the presented work is to synthesize ⁹⁹Tc^m-labeled dopamin D₂ receptor imaging agent 2-{1-[(1-Ethoxycarbonyl-2-mercapto-ethylamino)-methyl]}-3-{4-[(1-ethyl pyrrolidin-2-ylmethyl)-carbamoyl] -3-methoxy-5-bromo-phenylamino}-propylamino)-3-mercapto-propionic acid ethyl ester (⁹⁹Tc^m-m-Br-p-EBZM). The ECD was coupled with the benzamide derivative, N-[(1-ethyl-2-pyrrolidinealkyl)methyl]-4-acetylamino-5-bromium-2- methyloxygen-= benzol formamide, to yield m-Br-p-EBZM. The labeling compound was prepared through two steps of exchange labeling with ⁹⁹Tc^m-glucohetonate, and its stability was observed. Results show that m-Br-p-EBZM was synthesized for the first time, and was successfully labeled with ⁹⁹Tc^m with 98% of radiochemical purity, high stability and good liposolubility,. Hence ⁹⁹Tc^m-m-Br-p-EBZM may become a potential dopamine D₂ receptor imaging agent. (authors)

[en] Liaohe super-heavy crude oil was separated into its components, namely saturates, aromatics, resins, and asphaltenes (SARA), by the group separation method. Several solvents were used to extract different forms of metallic elements from crude oil. The metallic elements, such as calcium, nickel, iron and manganese, in crude oil, SARA and extract samples were analyzed by inductively coupled plasma atomic emission spectroscopy (ICP-AES). The results demonstrate that the contents of calcium, nickel, iron, and manganese gradually increase in saturates, aromatics, resins, and asphaltenes, suggesting that the abundance of the four metallic elements in asphaltenes is much higher than that in the other groups. For example, the content of calcium in asphaltenes reaches a maximum of 7,920 μg/g. Among the SARA components of Liaohe super-heavy crude oil, resins account for more than 50 wt%, suggesting that the total amount of the four metallic elements are higher in the resin component than in other components. The four metallic elements mainly exist in the form of organic metallic compounds in crude oil. Further analysis shows that calcium and manganese elements exist mainly as metal salts of petroleum acids, and the majority of the iron and all the nickel exist mainly as metalloporphyrin and non-metalloporphyrin compounds.

[en] ⁹⁹Tc^m-labeled dopamine D₂ receptor imaging agent ⁹⁹Tc^m-mEBZM was prepared and the biological evaluation was studied. ECD (L,L-ethyl cysteinate dimer) was conjugated with the benzamide derivative, which has high affinity with dopamine D₂ receptor, to yield ⁹⁹Tc^m-mEBZM. The biodistribution of ⁹⁹Tc^m-mEBZM in rats and mice was studied. The results showed that ⁹⁹Tc^m-mEBZM has higher stability in vitro and higher lipophilicity. The brain uptake of ⁹⁹Tc^m-mEBZM in mice is high (2.97%ID·g^-1 at 2 min), and the ratios of striatum/cerebellum both in rats and in mice were >1.55