[en] Radioimmunoassay (RIA) of parathyroid hormone (PTH) was started in 1963, using antibodies prepared with antigen of bovine parathyroid extracts, and in these days, RIA of PTH has been developed using various human-PTH fragments. However, many of these assays leave some problems in the sensitivity, reproducibility and specificity. Therefore, the authors developed the synthesis of h-PTH (1-84), and a new RIA system with it. (Auth.)
Source
Cohn, D.V. (Louisville Univ., KY (USA). Dept. of Oral Biology); Potts, J.T. Jr. (Massachusetts General Hospital, Boston (USA). Dept. of Medicine); Fujita, T. (Kobe Univ. (Japan). School of Medicine) (eds.); International congress series; no. 635; 491 p; ISBN 0-444-80590-7; 
; 1984; p. 36-37; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
; 1984; p. 36-37; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
[en] Radioimmunoassays for parathyroid hormone (PTH) have served in the diagnosis of hypercalcemic states and in secondary hyperparathyroidism. The reported results are greatly varying, because only 2-25% of the total plasma PTH is biologically active. The remaining PTH consists largely of inactive carboxyl-terminal fragments with a longer half-life than intact PTH (iPTH). The authors describe the results of plasma iPTH measurements with an amino-terminal radioimmunoassay, in which the antibody recognises only the intact, short-lived, biologically active PTH. (Auth.)
Source
Cohn, D.V. (Louisville Univ., KY (USA). Dept. of Oral Biology); Potts, J.T. Jr. (Massachusetts General Hospital, Boston (USA). Dept. of Medicine); Fujita, T. (Kobe Univ. (Japan). School of Medicine) (eds.); International congress series; no. 635; 491 p; ISBN 0-444-80590-7; ; 1984; p. 17-20; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
; 1984; p. 17-20; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
[en] A lot of research has been done for characterization of the regional specificity of radioimunnoassays for Parathyroid Hormone (PTH). The main portion of plasma PTH consists of biologically inactive fragments, with a long half-life compared to active PTH. In this article a midregion-specific radioimmunoassay for PTH is described, the non-specific binding values and plasma creatinine influence are discussed. Finally some plasma measurements of hypo-, hyperparathyroid and hypercalcemic patients are presented. (Auth.)
Source
Cohn, D.V. (Louisville Univ., KY (USA). Dept. of Oral Biology); Potts, J.T. Jr. (Massachusetts General Hospital, Boston (USA). Dept. of Medicine); Fujita, T. (Kobe Univ. (Japan). School of Medicine) (eds.); International congress series; no. 635; 491 p; ISBN 0-444-80590-7; ; 1984; p. 21-23; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
; 1984; p. 21-23; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
[en] Bone gamma-carboxyglutamic acid (gla)-containing protein (BGP) is a major constituent protein of bone matrix. The protein is synthesized by osteoblast-like cells in culture and is induced by 1,25-dihydroxyvitamin D. BGP has been demonstrated to circulate in plasma. From these data the hypothesis has developed that measurement of plasma BGP may have clinical utility as a marker of skeletal metabolism. This paper reviews the methods available for the development of radioimmunoassays (RIA) for human BGP (hBGP) and the potential clinical applications. (Auth.)
Source
Cohn, D.V. (Louisville Univ., KY (USA). Dept. of Oral Biology); Potts, J.T. Jr. (Massachusetts General Hospital, Boston (USA). Dept. of Medicine); Fujita, T. (Kobe Univ. (Japan). School of Medicine) (eds.); International congress series; no. 635; 491 p; ISBN 0-444-80590-7; ; 1984; p. 27-29; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
; 1984; p. 27-29; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983
No abstract available
Source
Cohn, D.V. (Louisville Univ., KY (USA). Dept. of Oral Biology); Potts, J.T. Jr. (Massachusetts General Hospital, Boston (USA). Dept. of Medicine); Fujita, T. (Kobe Univ. (Japan). School of Medicine) (eds.); International congress series; no. 635; 491 p; ISBN 0-444-80590-7; ; 1984; p. 46-47; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983; short communication.
; 1984; p. 46-47; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983; short communication.
No abstract available
Source
Cohn, D.V. (Louisville Univ., KY (USA). Dept. of Oral Biology); Potts, J.T. Jr. (Massachusetts General Hospital, Boston (USA). Dept. of Medicine); Fujita, T. (Kobe Univ. (Japan). School of Medicine) (eds.); International congress series; no. 635; 491 p; ISBN 0-444-80590-7; ; 1984; p. 34-35; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983; short communication.
; 1984; p. 34-35; Excerpta Medica; Amsterdam (Netherlands); 8. International conference on calcium regulating hormones; Kobe-shi (Japan); 16-24 Oct 1983; short communication.