[en] In order to investigate the effects of the Pauli blocking and internuclear potential on the total reaction cross section, we apply a microscopic simulation method called Antisymmetrized Molecular Dynamics to the optical limit of the Glauber model. We have analyzed reactions induced by d, ³He, α and ⁶He projectiles on ¹²C target over an energy range from 10 MeV/A to 1 GeV/A, are the calculated results show good agreement with experimental data. It is shown that each of these effects, which are ignored in the usual Glauber-type calculation, amounts to about 20% at low energy regions, however, large cancellations occur when we include both effects. (author)