[en] A HPLC system has been developed for carrier free and rapid delivery in a physiological buffer of the α-particle emitting bone-seeking radiopharmaceutical ²¹²Bi-DOTMP. ²⁰⁵Bi-DOTMP was synthesized and HPLC purified to mimic and visualize the deposition pattern in bony tissues of ²¹²Bi-DOTMP by autoradiography. Inhomogeneous bone deposits were found with the highest concentration in the bone matrix, the endosteum and in the growth zones of young mice. Analysis of urine samples showed that ²⁰⁵Bi-DOTMP was cleared as an intact complex