[en] We have developed a new strategy for the generation of patterned fluorescence images in polymer film. A fluorescent acetyl protected indole 6 was transformed to a nonfluorescent indigo dye 7 by UV irradiation. In addition, a t-Boc protected fluorescent indigo molecule 8 was also converted to a nonfluorescent indigo derivative 7 under a chemical amplification condition. Photomasked UV irradiation of the precursor molecules allowed efficient generation of patterned fluorescence images in polymer film. The strategy described in current investigation is believed to be an important addition to the fluorescent patterning technology