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💡 Maximizing Performance in Microplate Shaking: Understanding the Limits of 3mm Incubator Shakers 💡 3mm orbit incubator shakers have been the biggest trend in shaking in the past 5 years with some papers claiming (multiple fold in some cases) increases in Viable Cell Densities (VCDs) and product titers when shaking 1ml of culture volume in 96DW blocks at 3mm/1000rpm versus 50mm/300rpm. When working with incubator shakers, especially those with a 3mm shaking orbit, it's essential to match the equipment to the task at hand. These shakers are great for specific applications but come with limitations that can affect experimental outcomes if not understood. 🔬 Optimal Use for 96-Deepwell Plates 3mm orbit shakers are well-suited for larger filling volume cultures in 96-deepwell plates. The small shaking orbit aligns with the dimensions and geometry of these plates, ensuring proper mixing, phase alignment, and oxygen transfer rates (OTR/kLa) of cultures op to 1ml. ❌ Why They Fall Short with 24-Deepwell Plates Due to the larger diameter of a well of a 24-deepwell plate, the speed of the liquid cannot keep up with shaker speed resulting in an out of phase condition. This can be seen in the video below and will lead to: - Inadequate mixing, leaving dead zones in the wells. - Poor oxygen transfer efficiency (low OTR/kLa values). - Splashing and formation of aerosols. - Suboptimal growth or reaction conditions, leading to inconsistent or poor results. ⚠️ The Lesson: Not all shakers are created equal for every plate type. Matching your equipment to your experimental setup is critical for reproducibility and success. If you're using 24-deepwell plates, use shakers with a larger orbit, better suited to these plate dimensions. 3mm shakers are trendy, 50mm shakers are forever! #LabTips #ShakingOrbit #OTR #DeepwellPlates #Biotechnology #CHO #HEK #CellCulture