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Cellular in vitro models are crucial in preclinical research for understanding the pathologies and development of new drugs. However, due to the complex nature of the human body, obtaining accurate cell models can be difficult.   Tebubio, our European distributor, is delivering a webinar on the challenges of in vitro models, exploring the advantages of consolidating biological sample sourcing with a single, reliable partner, and sharing insights into the comprehensive solutions available for cultivating pertinent cellular models.   📅Tuesday 25th June 🕐10:00am BST | 11:00am CEST 💻Register here: https://rb.gy/3j3zel   #CellularResearch #InVitroModels #ResearchEfficiency

Hepatocytes offer a physiologically relevant model for human translation of drug clearance and drug-drug interaction studies, but cell membranes can limit the entry of certain compounds, complicating translation.   Permeabilized hepatocytes offer an alternative way to utilize hepatocytes in research, with completely permeabilized plasma membranes, and intact subcellular organelle membranes. This maintains similar enzymatic activity, while allowing researchers to freeze, store and reuse material that would otherwise be lost.   Published in The AAPS Journal, the paper explores a novel method for optimizing hepatocyte permeabilization using saponin and details their value in providing mechanistic insights into liver metabolism and drug interactions.   Read more: https://rb.gy/puc71x   #Hepatocytes #CellModels #DrugDiscovery

Hepatocytes offer a physiologically relevant model for human translation of drug clearance and drug-drug interaction studies, but cell membranes can limit the entry of certain compounds, complicating translation.   Permeabilized hepatocytes offer an alternative way to utilize hepatocytes in research, with completely permeabilized plasma membranes, and intact subcellular organelle membranes. This maintains similar enzymatic activity, while allowing researchers to freeze, store and reuse material that would otherwise be lost.   Published in The AAPS Journal, the paper explores a novel method for optimizing hepatocyte permeabilization using saponin and details their value in providing mechanistic insights into liver metabolism and drug interactions.   Read more: https://rb.gy/puc71x   #Hepatocytes #CellModels #DrugDiscovery

The evolution of cell biology has led to the development of many effective tools to analyze human tissue behavior, but these often rely on artificial environments for in vitro modelling and tissue engineering. ExVivoGel, our human-derived Extracellular Matrix, contains hormones to promote cell growth, regeneration, and differentiation, enabling the development of complex representative tumor models. Read more here: https://rb.gy/3kg3cy #TissueEngineering #CellModels #Oncology

In vivo testing is a critical stage of the drug discovery process, but conventional immortalized cell lines, despite being highly scalable, may not fully capture the intricacies of in vivo conditions, offering only a limited representation of human disease and physiology. Through the design and development of human-derived cell models, we are transforming the drug discovery process, combining representativity with thorough predictivity analysis. To find out more about the need for predictive drug activity assays, read our recent blog: https://rb.gy/rudk6h #DrugDiscovery #CellModels #Assays

Researchers have developed the first-of-its-kind multiplexed high throughput screening (HTS) assay, allowing simultaneous monitoring of drug-metabolizing enzymes, and paving the way for more efficient drug discovery.   Selective chemical inhibitors are crucial for identifying and characterizing drug-metabolizing enzymes that are involved in the elimination of drug candidates. Whilst human hepatocytes serve as the model system for assessing drug metabolism, containing various drug-metabolizing enzymes including selective inhibitors for major CYP enzymes, there are limited options for less common CYPs and non-CYPs.   Described in The AAPS Journal, the multiplexed HTS assay uses 20 substrate reactions of multiple enzymes to simultaneously monitor enzyme inhibition in a 384-well format. Combined with robotic systems, highly sensitive LC-MS/MS, and virtual screening of ~4600 compounds, the assay generated hits for many drug-metabolizing enzymes, with a rate far surpassing traditional HTS for biological targets.   Read the full paper: https://shorturl.at/iMP24   #DrugDiscovery #HighThroughputScreening #MultiplexedAssay #Enzymes

We’re pleased to announce our partnership with Biopredic International for the production of pooled suspension human hepatocytes.   Despite their crucial role in assessing drug metabolism and hepatic clearance, current hepatocyte cell models have a limited lifespan which hinders long-term studies. This agreement combines Biopredic’s know-how in cell pooling with our expertise and production capacity in sourcing primary hepatocytes to provide DMPK researchers with access to large batches of pooled suspension human hepatocyte cell models. The pooled hepatocytes have an increased lifespan of over 12 hours, increasing throughput capacities and reducing assay costs.   Learn more about the hepatocytes and read the full press release here: https://rb.gy/wuyfw8   #Hepatocytes #CellModels #DPMK #DrugDiscovery #Liver

In a recent study, researchers investigated the influence of an altered ECM microenvironment in Idiopathic Pulmonary Fibrosis (IPF), a severe condition characterized by abnormal lung tissue repair and fibroblast activity.   A 3D in vitro model was established using IPF and control lung ECM-derived hydrogels seeded with IPF or control lung-derived primary fibroblasts. The results revealed significant alterations in fiber organization and mechanical properties within the fibrotic microenvironment. IPF fibroblasts distinctly changed the topographical arrangement of collagen fibers in their 3D microenvironment, leading to greater stiffness in IPF hydrogels compared to control hydrogels. Additionally, control fibroblasts exhibited differential responses compared to IPF fibroblasts.   These findings illustrate the intricate interplay between fibroblasts and the human ECM in both fibrotic and control settings, and the influence of the microenvironment on fibroblast-driven ECM remodeling response.   Read the full paper here: https://shorturl.at/nuzFZ   #Fibrosis #Lung #Hydrogels #3D #InVitroModelling

The choice of attachment plates greatly impacts human and canine hepatocyte spheroid formation in preclinical drug development.   A study compared the performance of ten different 96- and 384-well microplate types for liver spheroid formation and long-term culture. All 96-well plates supported the formation of human liver spheroids but exhibited differences in size, geometry, and reproducibility while the performance of different 384-well plates was inconsistent. Ultra-low attachment plates demonstrated the best performance in the formation of liver spheroids exhibiting the highest viability, most relevant phenotypes, and the lowest variation in toxicity assays.    These findings offer valuable insights into the impacts of ultra-low attachment plates on liver spheroid metrics, facilitating evidence-based plate selection and promoting standardization and reproducibility of liver culture experiments.   Read the full paper: https://shorturl.at/aequW    #Liver #Spheroids 

📣 Exciting news – we have successfully isolated non-parenchymal cells (NPCs) together with hepatocytes on a large scale!   The liver is a complex organ composed of different cell types which, in addition to the main functional cells, hepatocytes, include NPCs. To accurately mimic the liver's physiological functions and responses, isolation of NPCs with hepatocytes is crucial.   Using our novel machine ex-vivo perfusion approach, we have generated 300 vials of hepatocytes (11mln/vial) and 100 vials of NPCs to facilitate liver-focused organ-on-a-chip research, such as studies of functional interactions between cell types, and high-throughput screening for drug discovery.   Interested in learning more? Get in touch: https://rb.gy/rxsawt   #OOC #DrugDevelopment #Hepatocytes