Vesiculab

Exploring RNA-containing extracellular vesicles in infection: in this review article, Kayo Schemiko Almeida, Suélen Andreia Rossi, and Lysangela Ronalte Alves at Fiocruz examined the intricate interactions between EV-associated RNA and host-pathogen dynamics across bacteria, parasites, and fungi, aiming to uncover molecular mechanisms underlying infectious diseases and identify potential therapeutic targets https://lnkd.in/eWX_Dww8 They also highlighted remaining challenges, including unravelling the mechanisms of RNA sorting into EVs in diverse microbial kingdoms, understanding their specific effects on recipient cells, and standardising EV isolation techniques. #extracellularvesicles #exosomes #infection #RNA #bacteria #Vesiculab

Developing a novel microarray analyzer for extracellular vesicles from maternal plasma: in their latest work, Malene Møller Jørgensen at Aalborg University, Prof. Sammar Marei at Braude Academic College and collaborators described and validated a straightforward, efficient in vitro diagnostics analyzer capable of predicting preeclampsia (PE) and preterm delivery (PTD) by assessing multiple EV surface markers. These markers are associated with crucial biological processes such as vascular remodelling and immune response, with PP13 and complement component 1q playing pivotal roles https://lnkd.in/e8R7snAh The microarray analyzer successfully identified surface markers linked to inflammation, vascular relaxation, and immune modulation for PE, as well as cell adhesion and general EV markers for PTD. An article co-authored by Rikke Bæk, Jenni Sloth, Rami Sammour, Adi Sharabi-Nov, Manu Vatish and Hamutal Meiri #extracellularvesicles #exosomes #preeclampsia #biomarkers #microarrays #Vesiculab

Investigating whether the protein corona on MSC-derived extracellular vesicles acts as a camouflage mechanism: in this commentary article, Enrico Ragni and Michela Taiana at IRCCS Ospedale Galeazzi-Sant’Ambrogio explored the concept of a protein corona enveloping EVs, focusing on findings from Liam-Or et al., which demonstrated the effects of protein corona formation on mesenchymal stromal cell-derived EVs (MSC-EVs). Specifically, the adsorption of proteins, primarily albumin, from the surrounding media onto the EV surface prior to isolation significantly enhanced their uptake by liver parenchymal cells while reducing macrophage incorporation https://lnkd.in/eNFMmX9W Additionally, this modification increased the EVs’ half-life within the circulatory system. These results suggest that engineering MSC-EVs with an albumin-enriched protein corona could serve as a strategic camouflage to promote selective uptake by non-phagocytic liver cells. #extracellularvesicles #exosomes #MSCs #proteincorona #Vesiculab

Evaluating the effect of UPF2 on CD81 levels in extracellular vesicles: in their latest work, Chaehwan Oh, Kyoung Mi Kim at Chungnam National University and collaborators explored the role of UPF2 in regulating EV biogenesis and composition. UPF2 was found to enhance the expression of CD81, a key EV membrane protein marker, as its silencing significantly reduced CD81 levels in both intracellular and secreted EVs https://lnkd.in/ej8hnQu9 Interestingly, while CD81 levels decreased, CD63 expression increased, without affecting EV size or overall production. However, UPF2 knockdown selectively reduced the production of CD81-positive EVs and diminished their uptake efficiency by recipient cells, highlighting UPF2's critical role in shaping EV functionality. An article co-authored by Krystyna Mazan-Mamczarz, Myriam Gorospe and Ji Heon Noh #extracellularvesicles #exosomes #biomarkers #EVuptake #Vesiculab

Analysing the impact of extracellular vesicle cargoes for diagnosing coronary artery disease: in this communication, Peter McGranaghan at Charité - Universitätsmedizin Berlin, Hargita Hegyesi at Semmelweis University and collaborators investigated the relationship between circulating EVs, hypoxia-related proteins, and traditional risk factors for life-threatening coronary artery disease (CAD) to identify novel, precise biomarkers. PYGM, clusterin, CPN1, and EVs were selected for their unique roles and diagnostic potential in CAD pathophysiology https://lnkd.in/eyFsNsUh PYGM, primarily involved in muscle energy metabolism, has been linked to ischemic conditions and metabolic dysfunction, suggesting its potential as an indicator of cardiovascular risk. An article co-authored by Éva Pallinger, Nóra Fekete, Pál Maurovich-Horvat, Zsófia Dóra Drobni, Béla Merkely MD, PhD, Luigi Menna and Edit Buzás #extracellularvesicles #exosomes #blood #biomarkers #flowcytometry #Vesiculab

Examining how the physicochemical properties and composition of lipid nanoparticles influence their interaction with the immune system: in this review article, Laura Catenacci, Maria Cristina Bonferoni at Università di Pavia and collaborators discussed the composition and physicochemical properties of LNPs, such as size, shape, surface charge, and protein corona formation, highlighting their influence on immune system reactivity. Their analysis provided a foundation for developing new formulations with an optimal efficacy-to-safety ratio https://lnkd.in/e-vhXwT6 They concluded that for non-immunotherapy applications, it was essential to fine-tune mRNA-LNP components to reduce immune activation and enhance safety. An article co-authored by Rachele Rossi, Francesca Sechi, Daniela Buonocore, Prof. Milena Lillina Sorrenti, Sara Perteghella and Marco Peviani #LNPs #proteincorona #lipids #nanoparticles #Vesiculab

Predicting depression by analysing glycosylation changes of vWF in circulating extracellular vesicles: in their latest work, Norihiro Yamada, Shin Nakagawa at Yamaguchi University and collaborators analysed glycosylation patterns in EVs isolated from plasma using size-exclusion chromatography, comparing patients with major depressive disorder (MDD) to age-matched healthy controls. Lectin blotting revealed significantly reduced levels of Wheat Germ Agglutinin (WGA)-binding glycosylations in patients with MDD during the depressive state compared to healthy controls and those in remission https://lnkd.in/ee_r4eB6 Proteome analysis identified von Willebrand factor (vWF) as a key WGA-binding protein. WGA-binding vWF antigen effectively distinguished MDD patients from HCs and depressive versus remission states within the same patients. An article co-authored by Kana Tominaga, Naoomi Tominaga, Ayumi Kobayashi, Chihiro Niino, Yuta Miyagi and Hirotaka Yamagata #extracellularvesicles #exosomes #biomarkers #depression #Vesiculab

Studying the effects of ethanol-exposed microglial exosomes on neurons: in their recent article, Prashant Tarale, PhD., Dipak Sarkar at Rutgers University and collaborators investigated whether postnatal alcohol exposure (PAE) triggers apoptosis of β-endorphin neurons and disrupts stress responses via microglial exosomes carrying elevated levels of the chemokine MCP1. Their findings revealed that PAE prompts microglia to release MCP1-enriched exosomes, activating MCP1/CCR2 signaling and inducing apoptosis in β-endorphin neurons https://lnkd.in/egVWat4i Furthermore, treatment with the MCP1-CCR2 antagonist RS504393 effectively prevented β-endorphin neuronal death, normalised stress hyperresponses, and mitigated anxiety-like behaviours in PAE rats. An article co-authored by Shaista Chaudhary and Sayani Mukherjee, Ph.D.(Physiology) #extracellularvesicles #exosomes #microglia #apoptosis #Vesiculab

Identifying parameters for sorting different RNA cargo into extracellular vesicles: in this preprint article, Ahmed Abdelgawad, Mona Batish at the University of Delaware and collaborators conducted a comprehensive genome-wide analysis to explore how factors such as GC%, size, exon count, structuredness, and coding potential influence the sorting and enrichment of circular and long linear RNAs into EVs. Their findings revealed that RNA size and structuredness play pivotal roles in RNA enrichment across all RNA classes https://lnkd.in/eEFi-Swi Notably, structuredness was identified as a key factor explaining the preferential enrichment of circular RNAs over their linear counterparts in EVs. An article co-authored by Yiyao Huang, Olesia Gololobova, Yanbao Yu, Kenneth Witwer and Vijay Parashar #extracellularvesicles #exosomes #circRNA #miRNA #enrichment #Vesiculab

Studying the biomolecular corona dynamics of extracellular vesicles: in their latest article, Angelo Musicò, Paolo Bergese at University of Brescia and collaborators developed a fluorescence correlation spectroscopy-based methodology to investigate biomolecular corona formation on EVs derived from human red blood cells and amniotic membrane mesenchymal stromal cells in human plasma https://lnkd.in/e5Nw7nk3 Their results revealed distinct corona dynamics between the two EV populations, and suggested that the biomolecular corona's dynamics are influenced by the EVs' cellular origin, highlighting an additional layer of heterogeneity. An article co-authored by Andrea Zendrini, Santiago Alberto Gimenez Reyes, Valentina Mangolini, Lucia Paolini, Miriam Romano, Andrea Papait, Antonietta Silini, Paolo Di Gianvincenzo, Arabella Neva, Marina Cretich†, Ornella Parolini, Camillo Almici, Sergio E. Moya and Annalisa Radeghieri #extracellularvesicles #exosomes #biomolecularcorona #nanoparticles #Vesiculab