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What is Chlorophyll?

Last Updated : 07 Jun, 2024
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Chlorophyll is the green pigment found in plants, algae, and cyanobacteria that is essential for photosynthesis. Studying the chlorophyll formula and structure helps us understand how plants capture light energy for photosynthesis. The structure of chlorophyll includes a porphyrin ring, which is a large, stable ring made of carbon and nitrogen atoms, with a magnesium ion at the centre.

In this article, we will cover the structure of chlorophyll and read about its uses, and types of chlorophyll in detail.

Chlorophyll Meaning

Chlorophyll refers to a group of green pigments found within the chloroplasts of plants, algae, and certain cyanobacteria. These pigments play a crucial role in photosynthesis, the process by which light energy is converted into chemical energy usable by living organisms.

Chlorophyll absorbs light most efficiently in the blue-violet and red parts of the electromagnetic spectrum, while reflecting green light, which gives plants their characteristic green color. This pigment is essential for the transformation of solar energy into a form that can be utilized by living organisms, sustaining the energy flow within ecosystems.

What-is-Chlorophyll

What is Chlorophyll

Chlorophyll is Present in

Chlorophyll are found in many organisms.

  • Plants:
    • Found in the chloroplasts of plant cells, especially in leaves, giving plants their green color.
  • Algae:
    • Present in the cells of both freshwater and marine algae, allowing them to produce energy through photosynthesis.
  • Cyanobacteria:
    • Prokaryotic organisms that contain chlorophyll and are significant for oxygen production.
  • Phytoplankton:
    • Microscopic organisms in oceans and freshwater bodies, including algae and cyanobacteria, crucial for the aquatic food web.
  • Certain Protists:
    • Some protists, like euglena, have chlorophyll and perform photosynthesis, contributing to primary production in various environments.
Chlorophyll Pigments

Structure of Chlorophyll

Chlorophyll is a green pigment found in the chloroplasts. It plays a key role in photosynthesis by capturing light energy from the sun. The structure of chlorophyll is complex and consists of several distinct components:

  1. Porphyrin Ring:
    • The core structure of chlorophyll is a porphyrin ring, a large, stable ring composed of carbon and nitrogen atoms. This ring structure is similar to the heme group found in hemoglobin but with a magnesium ion (Mg²⁺) at the center instead of iron.
  2. Magnesium Ion (Mg²⁺):
    • At the center of the porphyrin ring is a magnesium ion. This central magnesium ion is crucial for the chlorophyll molecule’s ability to capture light energy.
  3. Phytol Tail:
    • Attached to the porphyrin ring is a long, hydrophobic phytol tail. This tail anchors the chlorophyll molecule to the thylakoid membrane within the chloroplast, allowing it to be part of the photosynthetic complexes.
  4. Side Chains:
    • The porphyrin ring has various side chains, including methyl (CH₃), ethyl (C₂H₅), and formyl (CHO) groups. These side chains influence the absorption properties of chlorophyll, allowing it to capture light efficiently.

Chlorophyll Formula

The most common chlorophyll found in plants, chlorophyll a, has the following chemical formula:

C₅₅H₇₂O₅N₄Mg

Here’s a breakdown of the formula:

  • C₅₅: 55 carbon atoms
  • H₇₂: 72 hydrogen atoms
  • O₅: 5 oxygen atoms
  • N₄: 4 nitrogen atoms
  • Mg: 1 magnesium atom

Synthesis of Chlorophyll

The synthesis of chlorophyll is a complex biochemical process that occurs in the chloroplasts of plants, algae, and cyanobacteria. This process involves multiple steps and enzymes, ultimately leading to the production of chlorophyll, which is essential for photosynthesis. Here are the key stages of chlorophyll synthesis:

  1. Glutamate Pathway:
    • The synthesis begins with the amino acid glutamate, which is converted into 5-aminolevulinic acid (ALA) through a series of enzymatic reactions. This step is crucial and is regulated to control the overall rate of chlorophyll production.
  2. Formation of Porphobilinogen:
    • Two molecules of ALA condense to form porphobilinogen, a pyrrole ring. This reaction is catalyzed by the enzyme ALA dehydratase.
  3. Synthesis of Uroporphyrinogen III:
    • Four molecules of porphobilinogen are then assembled into a linear tetrapyrrole molecule, which cyclizes to form uroporphyrinogen III. This step is facilitated by the enzyme uroporphyrinogen III synthase.
  4. Conversion to Protoporphyrin IX:
    • Uroporphyrinogen III undergoes further modifications, including decarboxylation and oxidation, to form protoporphyrin IX. This intermediate is crucial as it is a precursor for both chlorophyll and heme biosynthesis.
  5. Incorporation of Magnesium:
    • The enzyme magnesium chelatase inserts a magnesium ion (Mg²⁺) into protoporphyrin IX to form magnesium protoporphyrin IX, a pivotal step that directs the pathway towards chlorophyll synthesis.
  6. Formation of Chlorophyllide:
    • Magnesium protoporphyrin IX is converted to protochlorophyllide through several steps involving enzymes such as magnesium-protoporphyrin IX monomethyl ester cyclase. Protochlorophyllide undergoes a light-dependent reduction to form chlorophyllide.
  7. Attachment of Phytol Tail:
    • Finally, the enzyme chlorophyll synthase attaches a phytol tail to chlorophyllide, producing chlorophyll a. Chlorophyll a can be further modified to form chlorophyll b and other types of chlorophyll.
  8. Regulation and Light Dependency:
    • The synthesis of chlorophyll is tightly regulated and can be influenced by factors such as light intensity, developmental stage, and environmental conditions. In higher plants, some steps, particularly the reduction of protochlorophyllide to chlorophyllide, are light-dependent, ensuring that chlorophyll synthesis is synchronized with exposure to light.

Types of Chlorophyll

The various different types of chlorophyll are:

Types  of Chlorophyll

Types of Chlorophyll

Chlorophyll A

Found in all higher plants, this is the primary pigment used in photosynthesis. It is also present in some algae, cyanobacteria, and anaerobic phototrophs. Chlorophyll a absorbs violet-blue and orange-red light, reflecting blue-green light.

Chlorophyll B

This pigment is found in green-growing plants and acts as an accessory pigment to chlorophyll a. It absorbs orange-red light and reflects yellow-green light. The chlorin ring of chlorophyll b has a CHO group, whereas chlorophyll a contains a CH3 group.

Chlorophyll C

Primarily found in marine algae such as brown algae, diatoms, and dinoflagellates. It has a unique porphyrin ring and can be classified into chlorophyll c1, c2, and c3, each with different chemical compositions and absorption rates.

Chlorophyll D

Present in red algae and cyanobacteria, which live in deep water and utilize red light for photosynthesis.

Chlorophyll E

A rare pigment found in some golden algae. It has been identified in xanthophytes (yellow-green algae).

Chlorophyll F

Recently discovered, chlorophyll F absorbs infrared light, which is beyond the visible range. Its function is still under investigation.

Uses of Chlorophyll

Chlorophyll has gathered attention as a health supplement for its potential benefits in various areas, including skincare, odor control, and cancer prevention.

  • Anti-aging Properties: Studies suggest that chlorophyllin gel application can reduce signs of photoaging, such as wrinkles and sun damage, potentially serving as an effective anti-aging remedy.
  • Acne Treatment: Chlorophyllin gel has shown promise in reducing facial acne and minimizing large pores, offering a natural alternative for those with mild to moderate acne.
  • Blood-building Properties: Due to its chemical similarity to hemoglobin, chlorophyll-rich wheatgrass juice may aid in treating hemoglobin deficiency disorders like anemia and thalassemia.
  • Wound-healing Properties: Historical research and recent reviews indicate that chlorophyllin promotes wound healing and prevents infections, making it a valuable adjunct in wound care management.
  • Cancer Treatment: Experimental studies on animals suggest that chlorophyllin could potentially prevent and slow down cancer growth, particularly in colon cancer and lung cancer cases.
  • Biological Use: Research has also explored chlorophyll’s role in biological processes, including its ability to facilitate the exposure of specific radio wire proteins, possibly implicating its involvement in plant defense mechanisms and oxidative stress responses.

Conclusion – Structure of Chlorophyll

Chlorophyll, the pigment responsible for the vibrant green color of plants, plays a vital role in photosynthesis, the foundation of life on Earth. Its complex structure allows it to capture sunlight and convert it into usable energy. Chlorophyll exists in various forms, each with slightly different light absorption properties that contribute to the overall photosynthetic process. While research continues on its potential health benefits, chlorophyll’s significance in sustaining life and ecosystems remains undeniable.

Also Read 

FAQs on Chlorophyll

What is Chlorophyll and its Function?

Chlorophyll is a green pigment found in plants that captures light energy for photosynthesis.

What does Chlorophyll do for Your Body?

In the body, chlorophyll aids in oxygen transport, wound healing, and potentially cancer prevention.

What is Chlorophyll Mainly Used For?

Chlorophyll is mainly used as a dietary supplement for its potential health benefits.

Can I Drink Chlorophyll Everyday?

Drinking chlorophyll daily is generally safe but may not be necessary for everyone.

What are Chlorophyll Benefits?

Chlorophyll benefits may include anti-aging, acne treatment, wound healing, and potentially cancer prevention



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