Analytical Outfitters, LLC

Fractionation reduced the polydispersity of this customer's material from Mw/Mn of 2.1 down to 1.3 on the first pass. after repeated auto-injections provided enough material, fractions were concentrated and run by triple detection GPC. 15-minute runtime. $80k full install cost with columns, installation and training. system components: modular, custom repeat auto-injector semi prep pump and degasser refractive index detector injection and fractionation valves Clarity control software with GPC module Shodex 20mm x 300mm mixed bed heating/cooling column thermostat oven the conical vials for fraction collection were chosen for low complexity because these fit the customers vacuum centrifuge format. easy transfer to a chilled 50mL format vacufuge. VICI Sykam GmbH DataApex Biotech Fluidics

how long can I keep my GPC standards? not this long! today is 8/27/24. one of these PEO standards is dated 11/9/16. that standard could be in the 3rd grade by now! if you want good data, be reasonable about your standards and columns. they are consumables. if you buy GPC / SEC standards from PSS Polymer Standards Service GmbH - A part of Agilent, or Malvern Panalytical -- good on you! you understand how important your data is. and you are willing to spend way, way more than you need to for standards. we can help spread that standard budget further.

Analytical Outfitters, LLC keeps your Viscotek Corporation and Malvern Panalytical GPC systems running past their End of Support date. 270 Dual Detector (LS + Viscometer) Refractive Index Detectors (Viscotek, Schambeck, Sykam) TDA 302 TDA 305 HT-GPC 350 GPCmax Solvent and Sampling Module Knauer 2600 UV-PDA Add-on OMNISEC Resolve / Reveal OK -- that last one is not actually past end of support. Officially. In fact they are still being manufactured in the UK. We have just run into too many customers in the US and Canada whose OMNISEC systems are inoperable, were not supported during the warranty period, or who just knew that Analytical Outfitters provides a higher level of expertise than the OEM. If you want to be able to send in data, get a confirmed remote diagnosis, and then experience one-visit resolution of your problems, that's we do. 7 years past End of Manufacture is the industry-standard for the analytical instrumentation field. Some companies keep things running a lot longer on a best-effort basis. PerkinElmer comes to mind. Agilent Technologies does a good job, but then the same parts that went into your 1100-series HPLC are the same ones that go into your 1200-series unit. What other companies do a good job keeping their older units running in the field?

making lemonade from lemons. this Malvern Panalytical Viscotek Corporation #GPC detector array was badly damaged at a resin manufacturing site after the scientist missed making a fluidic connection when installing new columns. our customer received a replacement TDA 302 from Analytical Outfitters, LLC at about 30% of list price, and we ♻️ the rest. proper electronics waste disposal is a part of our operations. scrap metal and plastic recycling made their way into the picture that day, too.

If you run Light Scattering experiments then contaminant particles are one of your worst enemies. for #GPC/#SEC experiments, inline filtration removes particles shed from the #chromatography column. your Waters | Wyatt Technology, Tosoh Bioscience - Separation & Purification Malvern Panalytical or Agilent Technologies systems can and do digitally filter out the spikes that particles cause as they pass through the flow cell. our filtration kits come designed for any mobile phase. PTFE, Nylon, CEllulose Acerate. 0.2 micron filter, with a coarse 10 micron stainless steel frit to catch larger contaminants like gels or fibers. these LS filters are placed ideally in two locations -- between injector and guard column, and again between the last Analytical column and your detector. if you recycle your organic mobile phase, then a third filter between solvent pump and injector is a must for maintaining your system hardware from solvent recycling mishaps. rebuild kits come 25 filters to a pack. replace the frits every 3 filter changes. most of my customers change filters once a month. aqueous buffers, every 2 weeks. we accept CC payments via an easy QuickBooks invoicing system. or POs for larger orders.

A "Narrow" Standard, you say? Your size exclusion #chromatography disagrees. For the purposes of multidetector #GPC, narrow standards have polydispersity indices (PDI, PD) of less than 1.10. If you indeed prepared a narrow standard and you seeing anything beyond the expected tailing or band broadening, then there are only a few diagnostic steps to consider based on our field experience: 1) your guard column / pre-column is contaminated 2) someone replaced a tubing section between the column and the first detector with wide-bore (> 0.25mm ID) tubing Guard columns / pre-columns are consumables. You can reverse-flush them if contaminated with a known bad sample. Follow manufacturer directions, and then reinstall and try again. Always return to a known good standard and overlay your narrow standard injections if you have doubts about whether there is non-ideal separation going on. Remember that GPC/SEC is about physical, size-based separation. Chemistry happens, but it should not be a major contributing mode of separation until you get to the small-molecule contaminants present in any sample. Ensure you keep the proper tubing on hand for maintenance. Where possible and affordable, use pre-cut tubing. Or learn to properly score and snap your own tubing to save a lot and enable on-the-fly updates. For general purpose non-UHPLC applications, use 0.25mm (0.01") internal diameter for flow path and 0.75mm (0.03") ID for post-detector flow to the waste bottle. For UHPLC, 0.125mm ID tubing for the flow path is more common.

PSA: Always run your standards in duplicate! While this looks like a 3-point injection volume linearity check, it is in fact a set of 3 programmed 100ul injections of a 72 kDa polystyrene in THF. This customer complained of wildly varying results. An overlay (available in all good chromatography software suites) gives an easy visual depiction of the problem. Quantification is easy enough by comparing peak area. Every IQOQ or system suitability check will have different thresholds. For #GPC / #SEC using a 200ul loop and partial loop fill set to a 100ul injection volume, I would expect better than 1% precision. Want to learn more about #polymer #chromatography #troubleshooting? Follow us for regular tips, tricks and updates. Doesn't matter if you use Agilent Technologies, Waters | Wyatt Technology, Tosoh Bioscience - Separations & Purification, Shimadzu Scientific Instruments, KNAUER or any other major brand. These systems all function on the same basic principles. Just like Click and Clack from Car Talk Digital used to do it: regardless of the make and model, with some basic information we can get to the root of the problem. If you're willing to give our ideas a try and come back with the results, we will drill down to the right answer 100% of the time.

you absolutely need to have a system (hardwar and/or procedural) in place to manage solvent use.

When should I replace my #GPC columns? One single narrow #standard will tell you everything you need to know. This user sent in some data from when they were using a 3-column set from Tosoh Bioscience - Separations & Purification back in 2022. Then we changed those out to a 2-column Agilent Technologies PLgel Olexis set. If all you are running are broad samples and standards, you can easily miss the peak tailing. Looking at the red (old) and purple (new) traces on the RALS (right angle light scattering) detector, you can easily see there is not only tailing but also significant dispersion. The OmniSEC software from Malvern Panalytical provides a the sigma and tau values when calibrating a method using a narrow standard. Along with an visual overlay of the narrow standards, the sigma and tau values provide numerical justification for when to change out your columns. Whether you are doing #protein SEC work with something like BSA or #polymer GPC work and using PS, PMMA, pullulan, PEO or any other narrow standard that Analytical Outfitters, LLC provides, you can always send in data for free-of-charge remote support. Even if the manufacturer won't support your system because it's more than 7 years past end of manufacture, we are here to support you. Factory-grade refurbished instrumentation. OEM repair parts. Preventive Maintenance kits. Service Level Agreements. Columns. Standards. Sample Preparation. From Analytical to Preparative, we have your #chromatography covered.

high temperature GPC is usually reserved for #polyolefins like #polypropylene and various PE grades like #hdpe or #lldpe. for QC labs, conventional calibration to a polystyrene standard curve is common. this system uses inline dRI, viscometer and two-angle light scattering, measuring Rg, Rh, branching and numerous further parameters. even though this advanced system from Malvern Panalytical / Viscotek Corporation was manufactured in 2013, this model still sports the lowest angle light scattering on the market, allowing direct measurement of molecular weight without the drift nfluence of column calibration. routine maintenance is where Analytical Outfitters, LLC comes in. need a new laser? replacement pump parts? a full overhaul after changing solvents? we provide this across the US, Canada and Europe.