Etaluma, Inc.

There is a preponderance of cell painting posters here at SBI2 and even live cell painting.

Many years ago we did a demonstration of bioluminescence microscopy with Promega’s NanoLuc® which offers “a luminescent signal 100X brighter than that of firefly or Renilla luciferase and with stable “glow-type:” kinetics.”  Our short and simple optics path along with advances in sensors allowed very reasonable exposures. One challenge is the darkness required. In our case, being inside the incubator can solve this. We have not seen much interest in this. I’m curious if this is because the only commercial luminescence microscope I could find was the discontinued Olympus LV200 and I don’t think it overlapped the release of NanoLuc®. Thanks for any comments. Here’s Promega’s article:  https://lnkd.in/eSxMW4hf #livecellimaging

I thoroughly enjoyed my lunch demo and discussion with Leanna Levine yesterday. Turns out we both grew up in St. Louis and were in adjacent labs at Wash U 40 years ago doing porphyrin synthesis! ALine Inc is a leader in microfluidics and Etaluma, Inc. has many Lab-on-a-Chip customers taking advantage of the open deck for plumbing and electrical connections. And if you are in Southern California, check out the brisket at Bad to the Bone BBQ at Ortega highway and the 5.

The authors of "Multiplex Single-Cell Bioprinting for Engineering of Heterogeneous Tissue Constructs with Subcellular Spatial Resolution" point out that "strategies to fabricate tissues with the exact same organization of individual cells in their native cellular microenvironment have remained virtually non-existent to date." They "demonstrate, for the first time, the ability to produce high-fidelity replicas of a patient’s annotated cancer biopsy with subcellular resolution...and report a method to spatially pattern single cells with an unprecedented level of spatial precision (1.3 µm between cells) and multi-typic cell heterogeneity (up to 8 phenotypes in a single print)." They use an Etaluma, Inc. microscope to monitor the single cell dispense. #livecellimaging https://lnkd.in/eNQwYCeJ

The highlight for me in our recent webinar "AI Assisted Tumoroid Viability Assays Using Widefield Microscopy", is this slide comparing a 10x confocal with our 10x wide field. The confocal consisted of 10 individual slices with a max z-projection display while ours is a single exposure, no post processing. Ours was taken in the cell–friendly incubator and the confocal on a stage. In this case the confocal scope used was twice the cost of our LS850 wide field scope and caused potentially 10x the phototox. If you missed the webinar, here is a link to view it: https://lnkd.in/gwUJrZEF #livecellimaging

I really enjoyed meeting with the "Translational Center for Barrier Microphysiological Systems" team at University of Rochester. Jim McGrath has as built a world class team for this initiative. A special thanks to Michael Klaczko and Stefano Begolo, PhD, EMBA, PMP as well as Lindsay Rathbun Wysocki of the microscope core and Dan Ahmad shown here in front of one of their Etaluma, Inc. microscopes. In this case with a rather rare 1.25x objective used to see an entire tendon on a chip in the Hani Awad lab. #livecellimaging

"The RHOA Mutation G17V Does Not Lead to Increased Migration of Human Malignant T Cells but Is Associated with Matrix Remodeling" The authors use microfluidic channels and collagen to measure the motility of cancer cells. "Polydimethylsiloxane (PDMS) chips with different types of microchannels were produced in molds provided by Dr Matthieu Piel. Straight channels with a diameter of 8 µm and a height of 10 µm were tested and determined to be most appropriate for HH and HuT-78 cells. Cell motility was monitored using a phase-contrast microscope (Lumascope LS720, Etaluma, Carlsbad, CA, USA) under incubator conditions for 20 h, with time-lapse images taken every 10 min in straight channels." https://lnkd.in/gjRn3qkh #livecellimaging

Going after cancer by removing iron. "mitochondrially targeted deferasirox (mitoDFX), a redox-active iron chelator that deprives cells of biologically active iron, as evidenced by a decrease in [Fe-S] cluster and heme-containing proteins. Notably, mitoDFX also depletes the major cellular antioxidant glutathione and induces lipid peroxidation, both of which are hallmarks of ferroptosis, resulting in selective induction of cell death in cancer cells." The authors used a single color live/dead assay. "Live-cell imaging was performed using a Lumascope S720 (Etaluma). ...SYTOX green, an impermeable nucleic acid stain, was used to detect the dead cells. Bright-field and green fluorescence (SYTOX) images were captured for 72 h at 3 h intervals."  #livecellimaging https://lnkd.in/gvqP3yri

It wasn't that long ago that we didn't have a 488nm(blue) LED. Here's a great story of an underdog scientist with heroic dedication to the problem who loses support from management, encounters PhD snobbery, and when he solves it, get's no compensation, goes to a competitor, gets sued, wins but gets it mostly reversed, and finally wins the Nobel prize.

Another open source #python tool for calcium and #livecellimaging. "DARTS (Deconvolution, Analysis, Registration, Tracking, and Shape normalization) provides state-of-the-art image postprocessing options like deep learning-based cell detection and tracking, spatio-temporal image deconvolution, and bleaching correction... Input: temporal image sequences, each representing a ROI containing a centered single-cell Output: analysis results at the single cell and the cell population level (text or Excel format)" https://lnkd.in/gHDJSJfv