Etaluma, Inc.

An ideal starter kit for tissue engineering would include easy-to-handle stem cell lines which would reproducibly form 3D vascularised connective tissue in one media which could last for months. Ashley Murphy developed this kit while opening a new lab and training three PhDs at School of Chemical Engineering UQ! Check out Ash's new preprint: using immortalised MSC and EC lines (ATCC) to develop consistent vasculogenesis strategies (with tiny error bars!). Ash's models could be triggered to undergo vascularisation simultaneous to adipogenesis for up to 31 days, forming lipid-laden microtissues for future human preclinical drug-testing. Now a Marie Curie Fellow in the Netherlands, Ash's hard work has developed standard protocols for a half-dozen more papers-in-progress in our lab. TermisEU2025 Tissue Engineering and Regenerative Medicine International Society, Inc. (TERMIS) Australasian Society for Stem Cell Research (ASSCR) Australasian Society for Biomaterials and Tissue Engineering International Society for Biofabrication Heart Foundation TERMIS SYIS-EU The University of Queensland

We have more and more customers coming to us because the competition have raised their prices dramatically. Etaluma, Inc. is proud to announce that there will be NO price increase for 2025 and our flagship LS850T automated microscope is still comfortably below $100K. Sometimes we can even provide multiple instruments for the price of one of theirs. Our LumaViewPro microscope control and analysis application is always free, including upgrades, forever. We welcome you to take a look what low overhead and honest pricing can deliver for your lab.

There is a preponderance of cell painting posters here at SBI2 and even live cell painting.

Many years ago we did a demonstration of bioluminescence microscopy with Promega’s NanoLuc® which offers “a luminescent signal 100X brighter than that of firefly or Renilla luciferase and with stable “glow-type:” kinetics.”  Our short and simple optics path along with advances in sensors allowed very reasonable exposures. One challenge is the darkness required. In our case, being inside the incubator can solve this. We have not seen much interest in this. I’m curious if this is because the only commercial luminescence microscope I could find was the discontinued Olympus LV200 and I don’t think it overlapped the release of NanoLuc®. Thanks for any comments. Here’s Promega’s article:  https://lnkd.in/eSxMW4hf #livecellimaging

I thoroughly enjoyed my lunch demo and discussion with Leanna Levine yesterday. Turns out we both grew up in St. Louis and were in adjacent labs at Wash U 40 years ago doing porphyrin synthesis! ALine Inc is a leader in microfluidics and Etaluma, Inc. has many Lab-on-a-Chip customers taking advantage of the open deck for plumbing and electrical connections. And if you are in Southern California, check out the brisket at Bad to the Bone BBQ at Ortega highway and the 5.

The authors of "Multiplex Single-Cell Bioprinting for Engineering of Heterogeneous Tissue Constructs with Subcellular Spatial Resolution" point out that "strategies to fabricate tissues with the exact same organization of individual cells in their native cellular microenvironment have remained virtually non-existent to date." They "demonstrate, for the first time, the ability to produce high-fidelity replicas of a patient’s annotated cancer biopsy with subcellular resolution...and report a method to spatially pattern single cells with an unprecedented level of spatial precision (1.3 µm between cells) and multi-typic cell heterogeneity (up to 8 phenotypes in a single print)." They use an Etaluma, Inc. microscope to monitor the single cell dispense. #livecellimaging https://lnkd.in/eNQwYCeJ

The highlight for me in our recent webinar "AI Assisted Tumoroid Viability Assays Using Widefield Microscopy", is this slide comparing a 10x confocal with our 10x wide field. The confocal consisted of 10 individual slices with a max z-projection display while ours is a single exposure, no post processing. Ours was taken in the cell–friendly incubator and the confocal on a stage. In this case the confocal scope used was twice the cost of our LS850 wide field scope and caused potentially 10x the phototox. If you missed the webinar, here is a link to view it: https://lnkd.in/gwUJrZEF #livecellimaging

I really enjoyed meeting with the "Translational Center for Barrier Microphysiological Systems" team at University of Rochester. Jim McGrath has as built a world class team for this initiative. A special thanks to Michael Klaczko and Stefano Begolo, PhD, EMBA, PMP as well as Lindsay Rathbun Wysocki of the microscope core and Dan Ahmad shown here in front of one of their Etaluma, Inc. microscopes. In this case with a rather rare 1.25x objective used to see an entire tendon on a chip in the Hani Awad lab. #livecellimaging