NanoImaging Services

We couldn’t agree more…. #cryoem #drugdiscovery

🧪 Working on #viral, #lipid, or #inorganic formulations? Cryo-TEM imaging and quantitative analysis can reveal the contents of your vial. 🔬 Phase appropriate #cryoTEM characterization can help at any stage of your pipeline, and reveal answers about formulation #stability, #morphology, size, #payload and more. 📩 Email us at nischr@nanoimagingservices.com to start planning your 2025 project! ▶️ https://lnkd.in/gTnWGWhp

💉 How does LNP size affect vaccine performance? 👩🔬 In "Impact of lipid nanoparticle size on mRNA vaccine immunogenicity”, Kimberly Hassett and colleagues from the Moderna team took a systematic approach to modifying the particle size of #mRNA-#LNPs #vaccine formulations encoding for a cytomegalovirus surface pentamer complex and for envelope glycoprotein gB by adjusting formulation process variables while keeping lipid composition constant. 🔬 #CryoEM nicely confirmed the intended increase in particle size as measured by NTA and DLS. Furthermore, cryo-EM images showed a wider size range as well as the presence of #multilamellar structures in the larger particle formulations. The particle surface was found to be less polar with increasing size, and after showing that total mRNA encapsulation did not correlate with LNP size measurements, the impact of LNP size on innate immunogenicity in mice and non-human primates was studied. 🧪 The researchers show that in mice LNPs smaller than 85–100 nm induced significantly lower immunogenicity with respect to the size interval of 100–200 nm, whereas in non-human primates all tested LNPs in the 60-150nm size range triggered a strong immune response when the LNPs were delivered intramuscularly, suggesting species-specific differences in LNP size sensitivity. 📑 Read the study: https://lnkd.in/gu4fgxTs 👉 Learn more about phase appropriate characterization of #lipidnanoparticles: https://lnkd.in/g5s7Pk7z

🔬 Gain an in-depth understanding into the anatomy of a #cryoEM grid & learn how sample prep can influence data collection. 🧪 Check out the wide variety of sample types phase appropriate #cryoTEM imaging and quantitative analysis can provide answers about.

🤔 How do you measure #blebs on #LNPs? 🔬 While DLS and Atomic Force Microscopy can give limited information, #cryoTEM imaging is the only reliable method to gain quantitative information on #blebbing in #lipidnanoparticles. 👀 Check out our whitepapers for an in depth study of LNP #morphology by cryo-TEM: https://lnkd.in/gPwFV8wM

🤔 Did you know as of 2021, about 530 drugs (30-35% of drugs available on the market) target #GPCRs? 🔬 About 60% of #GPCR structures have been solved by #singleparticleanalysis using #cryoEM. 👩🔬 Cryo-EM has enabled researchers to visualize GPCRs in complex with the trimeric G-proteins and in a variety of conformations. 📑 Check out more about GPCR characterization in our latest article: https://lnkd.in/gYDSQ6tY

🧪 Mixing systems, buffer, storage conditions (time, temperature, and more), and even flow rate can change the #morphology of #LNPs. Cryo-TEM imaging & analysis can provide insights into multiple CQAs in one study. 🔬 Through our collaboration with Phosphorex , #cryoTEM imaging studies revealed differences including size distribution, circularity, #payload encapsulation, presence of #blebbing, and more, that cannot be seen with other methods. 📚 Check out the data: https://lnkd.in/gPwFV8wM

🔬 NIS is proud to be on the cutting edge of #cryoEM, and pleased to offer #GMP compliant services and method validation in order to support you in regulatory submissions & demonstrating: 🧫 Batch-to-batch Reproducibility 🦠 Scale Up 💊 Comparability & #Biosimilars 🧪 #Stability Studies 📩 Send us a message at info@nanoimagingservices.com or request a consultation: https://lnkd.in/gTnWGWhp 👉 See the watchlist here: https://lnkd.in/eWZbk5uh

🗑️  The N- and C-degron pathways are essential processes in protein quality control, where E3 ligases identify specific sequences at the protein's ends to facilitate targeted degradation. The selectivity of the ubiquitin-proteasome system depends on specific interactions between E3 ligases and their substrates. 🔬  In the study "Structural Basis for C-degron Selectivity across KLHDCX Family E3 Ubiquitin Ligases," Scott et al. use #cryoEM, in combination with X-ray crystallography, to uncover key principles of C-degron recognition in the KLHDCX family of Cullin-RING ligases (CRLs). The structures reveal that these E3 ligases employ a common C-terminal anchor motif (R/F-S-R), which appears in distinct spatial locations, creating unique molecular environments that enable distinct specificities.   💡 This research provides valuable insights into how this family of #E3 #ligases contributes to the broader biological mechanisms of protein quality control. It also showcases how impactful it can be to use multiple #structuralbiology techniques in tandem. 📖  Read the full study here: https://lnkd.in/gruCzC9g 👩🔬  To explore how our cryo-EM services can help characterize your protein degrader complex, schedule a meeting with our experts: https://lnkd.in/gTnWGWhp

🧪 Cryo-grid prep is an incredibly intricate process, with many variables which can affect the quality of #cryoEM data. 🔬 An understanding of grid anatomy and the impact of grid materials, the air-water interface, and the vitrification process on sample behavior guide optimization strategies to mitigate common problems observed during imaging. 🧑🔬 Dive into this topic with a thoughtful primer on cryo-grid preparation from Dr. Emily Armbruster: https://lnkd.in/gBpm4eyb