Canton Biologics' DTEasy (Difficult to Express) platform is distinguished by its efficacy and versatility. We provide a comprehensive range of vectors meticulously engineered to address varying levels of expression difficulty. 📌 In one instance, regarding tissue plasminogen activator (t-PA): Challenges we faced: 1) the non-covalent complex formed between t-PA and its inhibitor exhibited a loosely structured nature, facilitating separation; 2) the traditional purification process for t-PA generally requires three to four steps to achieve purity exceeding 90%. Solutions: We developed a dual expression vector that co-expresses t-PA in conjunction with its inhibitor, complemented by an efficient one-step chromatographic purification process. Results: The final purified yield of t-PA reached an impressive 361.5 mg/L, surpassing current technological benchmarks by more than threefold. 📈 📩 Contact us: BD@cantonbio.com #BiotechInnovation #DTEasyPlatform #ProteinExpression
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Field Application Scientist at Acute Bioscience | Bioanalytical | Molecular Biology | Protein Engineering
Explore the mechanism of #protein unfolding with 𝐔𝐧𝐜𝐥𝐞. 𝐔𝐧𝐜𝐥𝐞 is an all-in-one platform for understanding thermal melting, aggregation, and sizing of biologics. Measuring thermal stability can be challenging for proteins that lack intrinsic #fluorescence. That’s where the “𝐓𝐦 𝐮𝐬𝐢𝐧𝐠 𝐒𝐘𝐏𝐑𝐎” application on 𝐔𝐧𝐜𝐥𝐞 comes into play! This app uses differential scanning fluorimetry (𝐃𝐒𝐅) with SYPRO dye to easily assess protein stability. Check out the technical note to learn more. #ThermalStability #DynamicLightScattering #lifescience #LifescienceResearch #mabs #bioanalytical #formulation #pharma #ThermalMelting
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Can we put a hydrophilic AND a lipophilic drug in ONE vehicle? Phospholipids in anisotropic drug delivery systems? Find out! 👇 Vincent Faivre from Université Paris-Saclay will talk about the role of phospholipids in anisotropic lipid-based #nanocarriers and their application as #drugdelivery system. More information on this topic: https://lnkd.in/eqHwUSQV #PLSymposium2024 📅 September 9-11, 2024 🌐 Heidelberg, Germany 🎯 Register: https://lnkd.in/emZgWsuf There is a growing interest in #asymmetry in the field of #nanotechnology for drug delivery. Anisotropy makes it possible to give original properties of mobility, encapsulation or interaction with biological media to the nanoparticles considered. #lipids #pharma #research #symposium
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Macrocyclic peptides, known for their enhanced specificity, stability, and bioavailability, are gaining prominence in peptide drug discovery. Automating head-to-tail cyclization on-resin, a traditionally challenging step, has the potential to boost recovery and minimize manual intervention in peptide synthesis. Take a closer look at our poster and learn more about automated peptide synthesis: https://hubs.ly/Q02Rsygl0 #peptidesynthesis #automation #biotage
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🚨 New Application Note💊 Enhance Drug Encapsulation with TDA Analysis 🧬 Are you working on drug delivery systems? This new study, conducted in collaboration with Advanced BioDesign, highlights the effectiveness of Taylor Dispersion Analysis (TDA) in evaluating drug encapsulation within lipid nanoemulsion (LNE) formulations. 👉 Unlike DLS, which is commonly used but limited in distinguishing size and encapsulation efficiency, TDA provides a more detailed analysis, offering better insight into encapsulation performance and size distribution. ✅ Key Findings: While DLS showed similar colloidal properties for two formulations, TDA revealed significant differences in drug encapsulation, proving the value of advanced characterization techniques. 🚀 Conclusion: TDA is a critical tool for optimizing lipid-based drug delivery systems, ensuring improved therapeutic efficacy. #DrugDelivery #NanoEmulsions #TDA #Pharma #Encapsulation #AdvancedBiodesign #Innovation https://lnkd.in/e6BPR-HQ
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How to move from polyclonal cell line to high quality clonal cell line ? 🔬 Clean Cells could help you on this critical step ! 🤝 Single cell cloning is an important step to generate high quality cell substrate for further biologics manufacturing. This precise method allows a single cell to be isolated and cultured from a heterogeneous population with high traceability. Imagine being able to control and guarantee the purity of your cell line with high precision! Single Cell Cloning opens the way to major advances in the production of biological drugs, by ensuring the clonality of cell lines and a better quality of your end products. This is a real revolution in our quest for effective and safe therapies for patients. In this fun video, find out how Clean Cells could support from your polyclonal cell line to a high quality GMP grade material ! Clean Cells, your reliable partner for your cell line development, from clonal selection to production and quality control ! 💡 For more information, please feel free to contact us > https://lnkd.in/e6zhFKse #SingleCellCloning #ClonalSelection #QualityControl #Onestopshopsolution #GMPcellsbanks Solentim VIPS®PRO
How to move from polyclonal cell line to high quality clonal cell line ? Clean Cells could help you on this critical step !
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Come learn about increasing productivity and reducing cost for large-scale LVV production!
✨ How does a structured fixed-bed bioreactor design address perfusion implementation challenges and minimize overall COGs for large-scale LVV production? ✨ How can a CDMO achieve consistent high LVV titers exceeding 6e6 IU/mL in 200L productions with its producer cell line? These questions will be answered by Floriane Cohen and Hannah McCauley in a free webinar on April 18th as they present a case study showing how high productivity and cost-efficient production of LVV is achieved through Genezen`s producer cell line innovation combined with Univercells Technologies' unique fixed-bed bioreactor design. Register today! https://lnkd.in/gGwqvagg #cellandgenetherapy #genetherapy #celltherapy #biotech #pharma #viralvectors #precisionmedicine #cdmo
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This paper discusses the crucial role of monoclonal #antibodies in drug development, emphasizing the importance of evaluating physical and chemical properties. The paper includes a study of 152 monoclonal antibodies that demonstrates a correlation between these properties and downstream processing. Colloidal stability is essential for developing safe and effective #protein based #therapeutics. Traditional stability monitoring techniques may not accurately capture dynamic processes like aggregation and degradation. The introduction of ARGEN offers a revolutionary approach by facilitating in-situ, real-time stability monitoring, manufacturing stress modeling, shelf-life determination, and rapid parallel analysis, enhancing formulation development efficiency and ensuring product quality and stability. #biopolymer #biotech #peptide https://lnkd.in/g739NM5D
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Executive Director, Conferences | Market Research, Conference Programming, Speaker Relationships
Advances in molecular format complexity and the need for higher protein concentrations in biotherapeutics present significant formulation challenges. Predicting protein behavior at high concentrations and the risks like high viscosity or aggregation remain challenging. Computational approaches are gaining popularity for biologics screening, while automation offers opportunities to accelerate formulation development. Join our 11th Annual Protein Stability & Formulation conference (https://lnkd.in/dtUXhBmG) at #PEGSEurope to learn from industry experts like Benjamin Weiche, Giuseppe Licari, Kannan Sankar, and Maurizio Baldassarre. #formulationdevelopment #insilico #computational https://lnkd.in/dtUXhBmG
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#Webinar now available on demand! Pure and simple: understanding #LNPanalytics for better #mRNA-based drugs #Ionizablelipids, the enabling technology of #lipidnanoparticles (#LNPs), are susceptible to impurities that cause loss of mRNA potency. Careful assessment of ionizable lipid purity throughout the #LNPmanufacturing process is vital. Adam Crowe (Senior Manager Analytical Development, Precision NanoSystems ULC (part of Cytiva)) will explore how #lipidpurity is carefully characterized using high resolution #massspectrometry coupled with a novel #fragmentationtechnology to both detect harmful N-oxide impurities at very low abundance, and distinguish them from other oxidated impurities. Watch this webinar at your convenience below:
Pure and simple: understanding LNP analytics for better mRNA-based drugs
insights.bio
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Read more about the recent advances that have made Abiologics possible from members of our founding team Mike Hamill and Jaclyn Dunphy.
Learn how #FlagshipFounded Abiologics is unlocking the next generation of biologics called Synteins™, a new biotherapeutic class with supranatural properties, revealing a world of new possibilities for patients. Jaclyn Dunphy and Mike Hamill explain how advances in generative protein design and optimization paired with automated chemical peptide synthesis are enabling this new approach to programmable biotherapeutics. Read more: https://bit.ly/4fy3sNf
