DNase (RNase-free) is an endonuclease that digests ssDNA, dsDNA and DNA in DNA-RNA complexes. The enzyme activity is strictly dependent on Ca²⁺ and is activated by Mg²⁺ and Mn²⁺ ions. Enzyme is purified from P.pastoris(K.phaffii) expressing bovine pancreas DNAse I gene. DNAse I may be used to degrade DNA in applications that are sensitive to the presence of RNAses. Application removal of contaminating genomic DNA from RNA samples DNA labeling by nick-translation studies of DNA-protein interactions by DNase I footprinting https://lnkd.in/gYkDH9pk #biotechnolgy #lifescience #DNAse #cut
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University of Limerick and Queen's University Belfast research looking at #AMR and specifically at the menace that is OXA-58. Gene transfer is one thing, but understanding expression of the gene in new host bacteria and its impact is another. Worth a read! https://lnkd.in/e9xYWVGr
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Understanding the transcriptome is essential for linking genome information to functions in plant metabolism and ultimately desired phenotypes. RNA-Seq is used to assess which genes are expressed within a plant or tissue, under which condition expression is activated or deactivated, and what the quantitative level of gene expression is at a given time. Find out more about the importance of RNA-Seq in plant and crop science: https://lnkd.in/dKv4v83N
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The Rural Life Conference (RLC) was very successful. we presented our research work on starch biosynthesis gene expression and high nighttime temperature on rice. where, starch biosynthesis is coordinated and eventual mechanism with multiple genes involvement. We quantified expression of different genes during grain filling stage of rice. We observed altered expressions of involved genes during grain fillings stage of rice.
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The Seurat single-cell RNA-seq analysis pipeline 2024 is an essential tool for analyzing gene expression data from individual cells. It’s designed to handle large datasets, perform clustering, identify different cell types, and explore relationships between cells. In this article, we’ll explore the basics, followed by code snippets to help you get started. Visit Us for More Information: https://lnkd.in/dPXBHcZc #SingleCellSequencing #scRNAseq #SeuratPipeline #GeneExpression #CellBiology #Bioinformatics #SingleCellAnalysis #RNASeqAnalysis #Seurat2024 #GenomicsResearch #DataScienceInBiology #LifeSciences #MolecularBiology #MachineLearningInBiology
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Download article: https://lnkd.in/gXfX2x36 Learn to identify and tackle the important, yet surmountable, challenges faced by modern gene expression studies to set your lab up for success. Download our latest article to uncover and tackle key challenges for successful studies. Maximize your lab's potential with actionable insights! #Research #LifeSciences #Biotechnology #GeneExpression
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Download article: https://lnkd.in/g6t6BvXV Curious about the future of gene expression research? Discover how cutting-edge technologies are pushing the boundaries of sensitivity, throughput, and spatial analysis. Explore the advancements and learn what's next for this vital field. Read our article to dive deeper! #GeneExpression #Biotechnology #FutureOfResearch
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How to calculate genotype and phenotype frequencies in the F1 generation An allele is one of two, or more, forms of a given gene variant. For example, the ABO blood grouping is controlled by the ABO gene, which has six common alleles. Nearly every living human's phenotype for the ABO gene is some combination of just these six alleles. The genotype of an organism is its complete set of genetic material. However, the term is often used to refer to a single gene or set of genes, such as the genotype for eye color. The genes partly determine the observable characteristics of an organism, such as hair color, height, etc. Youtube video: https://lnkd.in/d_XMQd9Q #nikolaysgeneticslessons
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How proteins are made? A codon is a trinucleotide sequence of DNA or RNA that corresponds to a specific amino acid. The genetic code describes the relationship between the sequence of DNA bases (A, C, G, and T) in a gene and the corresponding protein sequence that it encodes. The cell reads the sequence of the gene in groups of three bases. There are 64 different codons: 61 specify amino acids while the remaining three are used as stop signals. Youtube video: https://lnkd.in/datCSDzx #nikolaysgeneticslessons
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How to find if two genes are linked or not We can see if two genes are linked, and how tightly, by using data from genetic crosses to calculate the recombination frequency. By finding recombination frequencies for many gene pairs, we can make linkage maps that show the order and relative distances of the genes on the chromosome. Youtube video: https://lnkd.in/d9XB3ZeA #nikolaysgeneticslessons
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How to find phenotipic ratio in F2 generation A phenotypic ratio is a quantitative relation between phenotypes showing the number of times the frequency of one phenotype correlates with another. When a researcher would like to obtain the gene expression for generations of an organism, they use the phenotypic ratio obtained from a test cross. Youtube video: https://lnkd.in/d6XtM3Uw #nikolaysgeneticslessons
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