Autobio Diagnostics’ Post

Lahor Research & Molecular Diagnostic Laboratories Programme: Industrial Training #Biotechnology Date: 23-04-2024 Lecturer: Mr. Roland TOPIC: SFA(SEMINAL FLUID ANALYSIS) AND URINE MCS (MICROSCOPY) This is a series of test used to suspect infection caused by bacteria in the urine or semen. ☑️UTI (URINE PROCESSING) Things to observe when urine comes to the lab. a. Appearance b. colour c. culture the urine using either blood agar or Makconkay agar d. Urine microscopy e. Urine analysis using (combi 9)

🍄 PneumID Demonstrates Excellent Performance in PCP Diagnosis 📚 A study published in Journal of Fungi evaluated the performance of the OLM PneumID real-time PCR assay in diagnosing Pneumocystis pneumonia. The results showed excellent sensitivity and specificity, making PneumID a reliable tool for accurate PCP detection. Key findings: ✅ PneumID achieved a sensitivity of 96.9% and specificity of 95.1%. ✅ The assay provides a valuable tool for diagnosing PCP, especially when combined with serum (1-3)-β-D-Glucan testing. 🔗 Read the full case study here: https://lnkd.in/eWSUUHcR ➡️ Follow us on LinkedIn to stay informed about the latest research and developments in healthcare.  #Diagnostics #Laboratory #Pneumonia #PCR #InfectiousDiseases

Our latest review on the use of plants in oral candidiasis was just published in Critical Reviews in Microbiology. We conclude that adding "plants to a traditional antifungal (...) seems to have promising strategies. (...). However, "...on contrary to drugs, there is a critical lack of standardization on methodologies and protocols, which makes it difficult to compare data..." Congrats on the excellent work of my PhD student Marie MAZIERE and all the co-authors. https://lnkd.in/dSms4En4

I am delighted to share the #publication of my first-author research article, titled "Comparative Molecular Docking Studies of Selected Phytoconstituents on the Dopamine D3 Receptor (PDB ID: 3PBL) as Potential Anti-Parkinson’s Agents." I extend my sincere gratitude for the collaboration of co-authors Pallavi Dikshit and Namrata Kumari, as well as for the invaluable guidance provided by Manik Ghosh Sir. Parkinson’s disease is an idiopathic neurodegenerative disorder which is characterized by the degeneration of the neurons of substantia nigra, a part of the midbrain, regulating motor movement. Levodopa is a commonly prescribed medication for managing Parkinson's disease. Considering Levodopa as the standard, ten phytoconstituents were evaluated in this study through molecular docking against the dopamine D3 receptor to seek out potential inhibitors. Among them, Vasicol stood out with its favorable docking score and low toxicity profile, suggesting its potential as a promising candidate for the treatment of Parkinson's disease. #researcharticle #publication #parkinson #docking #medicinalchemistry #chemistry #journal #mdpi #chemistryproceedings #drugdiscovery #drugdevelopment #greenchemistry #pharmaceuticalindustry #pharmacy #pharmaceuticalchemistry #cadd #ShrimantiChakraborty

We are delighted to announce that on April 5, 2024, the results of an observational study comparing a brand-new point-of-care fluorescence immunoassay for determining circulating levels of vitamin D with ultra-high performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) were published in the prestigious peer-reviewed journal "Molecules." Vitamin D deficiency and insufficiency, which can lead to many pathological conditions, have become a widespread health problem, resulting in an increase in requests for determining circulating levels of 25-hydroxyvitamin D (25-OH-D), that is currently the main indicator of vitamin D status and it is performed by different assays. However, the analytical performance of these immunoassays is highly variable and does not necessarily provide comparable results. To address this issue, considerable efforts have been made to improve the metrological performance of this test to achieve comparable results across different methods and manufacturers. In particular, the AFIAS Vit D assay, a point-of-care fluorescence immunoassay based on a single cartridge with a lateral flow architecture and pre-filled with all the necessary reagents for the test, showed a good correlation with LC-MS/MS when running on both the Afias-1 and Afias-10 analyzers (Boditech and Menarini). Therefore, we can confidently assert that AFIAS-1 and AFIAS-10 are reliable instruments for accurately and rapidly measuring 25-OH-D levels. #innovation #healthcare #technology Full Pdf link: https://lnkd.in/dSHVAyXf

An interesting study on AFIAS Vitamin D you should take a look at #afias #menarinidiagnostics #vitd #healthcare

🔬 New Protocol Alert! Protocols for rapid identification of small-molecule metabolite ligands interacting with proteins By He et al. from Qingli Bie’s lab from Jining Medical University Summary: Metabolite-protein interactions have not been systematically studied due to a lack of effective techniques. This protocol focuses on identifying small-molecule metabolite ligands interacting with proteins. Highlights: Steps for mixing the sample with antibodies for immunoprecipitation and applying organic solvent to extract small-molecule metabolites. They then detail procedures for using high-resolution mass spectrometry to quantify small-molecule metabolites bound to proteins. Read the full protocol here: https://lnkd.in/euAdUdQx #STARProtocols #Cellpress #ResearchProtocol #Smallmoleculeligands 

🧫 Investigate the effect of sub-inhibitory concentrations of Nitrofurantoin, Ciprofloxacin, and Trimethoprim on in vitro biofilm formation in uropathogenic Escherichia coli (UPEC). This study explores antibiotic influence on bacterial resistance and biofilm development. 📖 Read the full paper here: https://lnkd.in/dmXz3fKB Authors: Whelan, S. et al. #Antibiotics #UropathogenicEcoli #BiofilmFormation #AntimicrobialResistance

Source: (The Analyst) Nanoparticle-based fractionation and stable isotope-labelled standards enhance reproducible quantitation of 270 human proteins via LC/MRM-MS, achieving a 44% increase in target detection and improved reproducibility (8.3% CV vs. 13.1% CV) compared to traditional methods. The technique shows higher protein concentrations and a compressed dynamic range, prompting further research into reference ranges and comparisons with established quantitation methods.

𝐀 𝐂𝐨𝐦𝐩𝐥𝐞𝐭𝐞 𝐆𝐮𝐢𝐝𝐞 𝐨𝐟 #Clinical_Reference_Laboratory [𝐏𝐃𝐅 𝐆𝐮𝐢𝐝𝐞] 📚 𝐆𝐞𝐭 𝐏𝐃𝐅 𝐒𝐚𝐦𝐩𝐥𝐞 𝐂𝐨𝐩𝐲: @ https://shorturl.at/sSBpI The clinical reference laboratory market size is projected to reach US$ 74.08 billion by 2031 from US$ 45.35 billion in 2023. The #market is expected to register a #CAGR of 6.3% during 2023–2031. The use of artificial intelligence in clinical reference #laboratories is likely to remain a key trend in the market. #ClinicalLaboratory #ReferenceLaboratory #LabTesting #DiagnosticTesting #MedicalLaboratory #ClinicalDiagnostics #HealthcareInnovation #PrecisionMedicine #MolecularDiagnostics #LabManagement #Pathology #HealthcareTechnology #MedicalTesting #Biotechnology #LaboratoryServices