Bio-Connect BV’s Post

Here’s another gem from Derek Lowe's "In the Pipeline" series. An imagined 1990s dialogue between a Section Head and a Bench Chemist in med-chem captures how revolutionary bifunctional molecules for protein degradation might have seemed. First validated in 2001 and boosted by 2012 VHL ligand discoveries, this concept faced skepticism and challenges. Fast forward, bifunctional protein degraders entered clinical trials in 2019, and now, over 18 targeted degraders, including PROTACs and molecular glues, are being tested. Article: https://lnkd.in/gBamMpQ7 #DrugDevelopment #ProteinDegradation #ClinicalTrials

Modified and unmodified oligonucleotides are efficiently separated and purified through ion pair reversed phase (IP-RP) separations. Downstream purification of oligos presents challenges due to a variety of reasons, for example impurities can be very similar to the target molecules. In this application note, we highlight the use of a batch-tested preparative column specifically designed for oligos, combined with HFIP-free mobile phase. This strategy provides a new level of assurance for the development of high throughput, high recovery purifications of CRISPR sgRNA, siRNA and oligo-conjugates. Read the application note here: https://bit.ly/3ZBZfCL

Precision in proteomics starts with the right tools. Spectrus offers cutting-edge solutions for Tandem Mass Tag Labeling, Stable Isotope Labeling, and Label-Free Quantitation. Want to know how we can help you? Our solutions, instrumentation, and technologies are waiting for you. Visit us today. ➡️️ https://lnkd.in/duaASGQ #AnalyticalChemistry #MassSpectrometry #LiquidChromatography #Proteomics #Metabolomics #Glycomics #Lipidomics #Epigenetics #FragmentScreening #PolymerAnalysis

Are you heading to #AMPEurope2024 this week? Make sure to say hello to LGC Biosearch Technologies and find out more about what they do with molecular diagnostics. https://ow.ly/luyb50SfbLV #ScienceforaSaferWorld

🔴 On-Demand #Webinar: Automated #Protein Structure Analysis Using DIA Based Hydrogen Deuterium Exchange #Mass #Spectrometry 🔴Join us for an on-demand webinar featuring Dr. David Schriemer from the University of Calgary, where he shares insights into innovative protein analysis methods.   🔴Discover a #data-independent #acquisition-based HX-MS workflow that enhances #efficiency and precision in #protein structure analysis. Dr. Schriemer will demonstrate its application in characterizing drug-binding interactions, offering valuable insights for biotherapeutics development. 🔔 Register here ❗ https://lnkd.in/dfZgsrUt #thermo #thermofisher #orbitrap #orbitrapastral #proteomics

Our co-founder and CSO Jan Kubicek had the opportunity to share some insights at Discovery on Target 2024 about one of the biggest challenges in membrane protein research: stabilization. In our talk, we discussed how next-generation NativeMP copolymers are paving the way for more efficient stabilization of membrane proteins into native nanodiscs, preserving their natural environment and functionality. If you’re working in biotherapeutics or are interested in membrane protein research, this approach could be a game changer! Check out the full talk here: https://lnkd.in/eKZ8ngFM

IMiD small molecule library ChemDiv’s collection of imide-like compounds for the discovery of PROTAC and molecular glue molecules contains 2,000 entries. Imide-like compounds provide the scientific background for discovering and developing PROTACs and molecular glue drug compounds, two innovative strategies in targeted protein degradation. These compounds act as crucial connectors or "glues" that facilitate the interaction between target proteins and the cellular degradation machinery via engagement with E3 ligase. https://lnkd.in/eTEBxRrY #IMiD

Challenges with Host Cell Protein Analysis? In this recent paper from scientists at Merck, they utilize the Evosep One coupled to a Bruker timsTOF Pro 2 to overcome the challenges of host cell protein (HCP) analysis. By leveraging diaPASEF, they were able to reveal the presence of more than 600 additional HCPs previously missed by DDA PASEF. In addition to improved characterization of the sample, they were able to increase throughput and eliminate carryover! Taku Tsukidate, Alyssa Stiving, Shannon Rivera, Ansuman Sahoo, Sri Madabhushi, Xuanwen Shawn Li Take a look at the full manuscript 👇 👇👇 https://lnkd.in/e4QkQSyy To learn more about the Evosep One, visit our website evosep.com or reach out to me for additional information. #Evosep, #Bruker, #Hostcellproteins, #hcp,

DNA Genotek Inc. and Twist Bioscience demonstrate a high-quality next generation sequencing library preparation and target enrichment solution using a streamlined workflow, from saliva sample to sequencer in under 8 hours. The prepIT™•Q2A reagent enables a rapid, liquid-based cleanup of saliva samples collected with Oragene™•Dx and ORAcollect™•Dx devices. It is an ideal solution for laboratories wanting to scale up their sample throughput — from saliva sample directly to library preparation. To read the protocol or request samples of prepIT™•Q2A reagent for your trial, please go to: https://lnkd.in/ebBwVSUE

Take a look at the Cell Chemical Biology paper where we present a cohort of mechanistic assays (NanoBRET™ Target Engagement NLRP3 Assay, the Caspase-Glo® 1 Inflammasome Assay and the Lumit® Active IL-18 Immunoassay) to query direct NLRP3 engagement in cells, and functionally interrogate different nodes of NLRP3 pathway activity. Check out: https://bit.ly/3TV4mur #assays #immunoassay #NanoBRET